Induction of in vitro roots cultures of Thypha latifolia and Scirpus americanus and study of their capacity to remove heavy metals

We have established the conditions to obtain in vitro root cultures of Thypha latifolia and Scirpus americanus and have investigated their capacity to remove Pb(II), Mn(II) and Cr(III) from the culture medium. The best conditions for the in vitro culture growth were: an inoculum of 0.2 g of T. latif...

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Autores principales:	Santos-Díaz,María del Socorro, Barrón-Cruz,María del Carmen, Alfaro-De la Torre,María Catalina
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2007
Materias:	heavy metals in vitro root cultures phytoremediation Scirpus americanus Thypha latifolia
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000300009
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spelling	oai:scielo:S0717-345820070003000092007-11-05Induction of in vitro roots cultures of Thypha latifolia and Scirpus americanus and study of their capacity to remove heavy metalsSantos-Díaz,María del SocorroBarrón-Cruz,María del CarmenAlfaro-De la Torre,María Catalina heavy metals in vitro root cultures phytoremediation Scirpus americanus Thypha latifolia We have established the conditions to obtain in vitro root cultures of Thypha latifolia and Scirpus americanus and have investigated their capacity to remove Pb(II), Mn(II) and Cr(III) from the culture medium. The best conditions for the in vitro culture growth were: an inoculum of 0.2 g of T. latifolia roots and 0.05 g of S. americanus roots (fresh weight), Murashige-Skoog medium and 2 mg L-1of indolacetic acid. The T. latifolia and S. americanus root cultures were cultivated onto media containing Cr (15 µg L-1), Pb (60 µg L-1) or Mn (1.8 mg L-1). Both species were able to remove Pb and Cr near to 100% and 71-100% of Mn from the medium solution during the 6-8 days of experimentation. According to metal concentrations removed from the medium containing the growing root mass, the in vitro root culture of S. americanus can be considered as an accumulator for Pb (157.73 µg g-1), Cr (55.6 µg g-1) and Mn (5000 µg g-1).info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.10 n.3 20072007-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000300009en10.4067/S0717-34582007000300009
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	heavy metals in vitro root cultures phytoremediation Scirpus americanus Thypha latifolia
spellingShingle	heavy metals in vitro root cultures phytoremediation Scirpus americanus Thypha latifolia Santos-Díaz,María del Socorro Barrón-Cruz,María del Carmen Alfaro-De la Torre,María Catalina Induction of in vitro roots cultures of Thypha latifolia and Scirpus americanus and study of their capacity to remove heavy metals
description	We have established the conditions to obtain in vitro root cultures of Thypha latifolia and Scirpus americanus and have investigated their capacity to remove Pb(II), Mn(II) and Cr(III) from the culture medium. The best conditions for the in vitro culture growth were: an inoculum of 0.2 g of T. latifolia roots and 0.05 g of S. americanus roots (fresh weight), Murashige-Skoog medium and 2 mg L-1of indolacetic acid. The T. latifolia and S. americanus root cultures were cultivated onto media containing Cr (15 µg L-1), Pb (60 µg L-1) or Mn (1.8 mg L-1). Both species were able to remove Pb and Cr near to 100% and 71-100% of Mn from the medium solution during the 6-8 days of experimentation. According to metal concentrations removed from the medium containing the growing root mass, the in vitro root culture of S. americanus can be considered as an accumulator for Pb (157.73 µg g-1), Cr (55.6 µg g-1) and Mn (5000 µg g-1).
author	Santos-Díaz,María del Socorro Barrón-Cruz,María del Carmen Alfaro-De la Torre,María Catalina
author_facet	Santos-Díaz,María del Socorro Barrón-Cruz,María del Carmen Alfaro-De la Torre,María Catalina
author_sort	Santos-Díaz,María del Socorro
title	Induction of in vitro roots cultures of Thypha latifolia and Scirpus americanus and study of their capacity to remove heavy metals
title_short	Induction of in vitro roots cultures of Thypha latifolia and Scirpus americanus and study of their capacity to remove heavy metals
title_full	Induction of in vitro roots cultures of Thypha latifolia and Scirpus americanus and study of their capacity to remove heavy metals
title_fullStr	Induction of in vitro roots cultures of Thypha latifolia and Scirpus americanus and study of their capacity to remove heavy metals
title_full_unstemmed	Induction of in vitro roots cultures of Thypha latifolia and Scirpus americanus and study of their capacity to remove heavy metals
title_sort	induction of in vitro roots cultures of thypha latifolia and scirpus americanus and study of their capacity to remove heavy metals
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2007
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000300009
work_keys_str_mv	AT santosdiazmariadelsocorro inductionofinvitrorootsculturesofthyphalatifoliaandscirpusamericanusandstudyoftheircapacitytoremoveheavymetals AT barroncruzmariadelcarmen inductionofinvitrorootsculturesofthyphalatifoliaandscirpusamericanusandstudyoftheircapacitytoremoveheavymetals AT alfarodelatorremariacatalina inductionofinvitrorootsculturesofthyphalatifoliaandscirpusamericanusandstudyoftheircapacitytoremoveheavymetals
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Induction of in vitro roots cultures of Thypha latifolia and Scirpus americanus and study of their capacity to remove heavy metals

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