Trametes versicolor growth and laccase induction with by-products of pulp and paper industry
The cultivation of Trametes versicolor for laccase production and cell growth were strongly dependent on experimental conditions namely physical and chemical parameters as well as nutrient availability and inducer stimulation. Biomass growth was compared for a rich medium and for a defined medium in...
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Pontificia Universidad Católica de Valparaíso
2007
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oai:scielo:S0717-345820070003000122007-11-06Trametes versicolor growth and laccase induction with by-products of pulp and paper industryRebelo Barreto Xavier,Ana MariaMora Tavares,Ana PaulaFerreira,RitaAmado,Francisco inducers laccase pulp and paper by-products Trametes versicolor The cultivation of Trametes versicolor for laccase production and cell growth were strongly dependent on experimental conditions namely physical and chemical parameters as well as nutrient availability and inducer stimulation. Biomass growth was compared for a rich medium and for a defined medium in two different temperatures. The best temperature was 28ºC and the maximum specific growth rates were µmax = 0.083 h-1 for the rich medium and µmax = 0.043 h-1 for the defined medium. It was clearly shown that laccase production is not associated with cell growth, indicating that this ligninolytic enzyme must be produced in the defined medium by a secondary metabolism. In order to obtain laccase induction, addition of solid lignin, lignosulphonates, veratryl alcohol, xylidine and ethanol was tested at different concentrations. To optimise laccase activity, the combined effect of inducer addition and simultaneously glucose suppression was studied. The best result for laccase induction (1240 U/L) was obtained with solid lignin, a by-product of pulp and paper industry and the higher laccase activity attained (1583 U/L) was obtained with the combined effect of xylidine addition and glucose suppression.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.10 n.3 20072007-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000300012en10.4067/S0717-34582007000300012 |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| topic |
inducers laccase pulp and paper by-products Trametes versicolor |
| spellingShingle |
inducers laccase pulp and paper by-products Trametes versicolor Rebelo Barreto Xavier,Ana Maria Mora Tavares,Ana Paula Ferreira,Rita Amado,Francisco Trametes versicolor growth and laccase induction with by-products of pulp and paper industry |
| description |
The cultivation of Trametes versicolor for laccase production and cell growth were strongly dependent on experimental conditions namely physical and chemical parameters as well as nutrient availability and inducer stimulation. Biomass growth was compared for a rich medium and for a defined medium in two different temperatures. The best temperature was 28ºC and the maximum specific growth rates were µmax = 0.083 h-1 for the rich medium and µmax = 0.043 h-1 for the defined medium. It was clearly shown that laccase production is not associated with cell growth, indicating that this ligninolytic enzyme must be produced in the defined medium by a secondary metabolism. In order to obtain laccase induction, addition of solid lignin, lignosulphonates, veratryl alcohol, xylidine and ethanol was tested at different concentrations. To optimise laccase activity, the combined effect of inducer addition and simultaneously glucose suppression was studied. The best result for laccase induction (1240 U/L) was obtained with solid lignin, a by-product of pulp and paper industry and the higher laccase activity attained (1583 U/L) was obtained with the combined effect of xylidine addition and glucose suppression. |
| author |
Rebelo Barreto Xavier,Ana Maria Mora Tavares,Ana Paula Ferreira,Rita Amado,Francisco |
| author_facet |
Rebelo Barreto Xavier,Ana Maria Mora Tavares,Ana Paula Ferreira,Rita Amado,Francisco |
| author_sort |
Rebelo Barreto Xavier,Ana Maria |
| title |
Trametes versicolor growth and laccase induction with by-products of pulp and paper industry |
| title_short |
Trametes versicolor growth and laccase induction with by-products of pulp and paper industry |
| title_full |
Trametes versicolor growth and laccase induction with by-products of pulp and paper industry |
| title_fullStr |
Trametes versicolor growth and laccase induction with by-products of pulp and paper industry |
| title_full_unstemmed |
Trametes versicolor growth and laccase induction with by-products of pulp and paper industry |
| title_sort |
trametes versicolor growth and laccase induction with by-products of pulp and paper industry |
| publisher |
Pontificia Universidad Católica de Valparaíso |
| publishDate |
2007 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000300012 |
| work_keys_str_mv |
AT rebelobarretoxavieranamaria trametesversicolorgrowthandlaccaseinductionwithbyproductsofpulpandpaperindustry AT moratavaresanapaula trametesversicolorgrowthandlaccaseinductionwithbyproductsofpulpandpaperindustry AT ferreirarita trametesversicolorgrowthandlaccaseinductionwithbyproductsofpulpandpaperindustry AT amadofrancisco trametesversicolorgrowthandlaccaseinductionwithbyproductsofpulpandpaperindustry |
| _version_ |
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