A simple method for isolation of genomic DNA from fresh and dry leaves of Terminalia arjuna (Roxb.) Wight and Arnot

Current protocols for isolation of genomic DNA from Terminalia arjuna have their own limitations due to the presence of high content of gummy polysaccharides and polyphenols. DNA isolated by these protocols is contaminated with a yellowish, sticky and viscous matrix. In our modified DNA isolation me...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Deshmukh,Vishal P, Thakare,Prashant V, Chaudhari,Uddhav S, Gawande,Prashant A
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2007
Materias:	Ayurveda DNA extraction medicinal plant Terminalia species
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000300014
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0717-34582007000300014
record_format	dspace
spelling	oai:scielo:S0717-345820070003000142007-11-06A simple method for isolation of genomic DNA from fresh and dry leaves of Terminalia arjuna (Roxb.) Wight and ArnotDeshmukh,Vishal PThakare,Prashant VChaudhari,Uddhav SGawande,Prashant A Ayurveda DNA extraction medicinal plant Terminalia species Current protocols for isolation of genomic DNA from Terminalia arjuna have their own limitations due to the presence of high content of gummy polysaccharides and polyphenols. DNA isolated by these protocols is contaminated with a yellowish, sticky and viscous matrix. In our modified DNA isolation method polysaccharides and polyphenols are removed prior to the precipitation of the DNA. Then the genomic DNA was precipitated using isopropanol. This protocol yielded a high molecular weight DNA isolated from fresh as well as dry leaves of T. arjuna, which was free from contamination and colour. Isolated DNA can be used for restriction digestion and PCR amplification. The main objective of the present protocol is to provide a simple method of isolation of DNA, using in house prepared reagentsinfo:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.10 n.3 20072007-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000300014en10.4067/S0717-34582007000300014
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Ayurveda DNA extraction medicinal plant Terminalia species
spellingShingle	Ayurveda DNA extraction medicinal plant Terminalia species Deshmukh,Vishal P Thakare,Prashant V Chaudhari,Uddhav S Gawande,Prashant A A simple method for isolation of genomic DNA from fresh and dry leaves of Terminalia arjuna (Roxb.) Wight and Arnot
description	Current protocols for isolation of genomic DNA from Terminalia arjuna have their own limitations due to the presence of high content of gummy polysaccharides and polyphenols. DNA isolated by these protocols is contaminated with a yellowish, sticky and viscous matrix. In our modified DNA isolation method polysaccharides and polyphenols are removed prior to the precipitation of the DNA. Then the genomic DNA was precipitated using isopropanol. This protocol yielded a high molecular weight DNA isolated from fresh as well as dry leaves of T. arjuna, which was free from contamination and colour. Isolated DNA can be used for restriction digestion and PCR amplification. The main objective of the present protocol is to provide a simple method of isolation of DNA, using in house prepared reagents
author	Deshmukh,Vishal P Thakare,Prashant V Chaudhari,Uddhav S Gawande,Prashant A
author_facet	Deshmukh,Vishal P Thakare,Prashant V Chaudhari,Uddhav S Gawande,Prashant A
author_sort	Deshmukh,Vishal P
title	A simple method for isolation of genomic DNA from fresh and dry leaves of Terminalia arjuna (Roxb.) Wight and Arnot
title_short	A simple method for isolation of genomic DNA from fresh and dry leaves of Terminalia arjuna (Roxb.) Wight and Arnot
title_full	A simple method for isolation of genomic DNA from fresh and dry leaves of Terminalia arjuna (Roxb.) Wight and Arnot
title_fullStr	A simple method for isolation of genomic DNA from fresh and dry leaves of Terminalia arjuna (Roxb.) Wight and Arnot
title_full_unstemmed	A simple method for isolation of genomic DNA from fresh and dry leaves of Terminalia arjuna (Roxb.) Wight and Arnot
title_sort	simple method for isolation of genomic dna from fresh and dry leaves of terminalia arjuna (roxb.) wight and arnot
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2007
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000300014
work_keys_str_mv	AT deshmukhvishalp asimplemethodforisolationofgenomicdnafromfreshanddryleavesofterminaliaarjunaroxbwightandarnot AT thakareprashantv asimplemethodforisolationofgenomicdnafromfreshanddryleavesofterminaliaarjunaroxbwightandarnot AT chaudhariuddhavs asimplemethodforisolationofgenomicdnafromfreshanddryleavesofterminaliaarjunaroxbwightandarnot AT gawandeprashanta asimplemethodforisolationofgenomicdnafromfreshanddryleavesofterminaliaarjunaroxbwightandarnot AT deshmukhvishalp simplemethodforisolationofgenomicdnafromfreshanddryleavesofterminaliaarjunaroxbwightandarnot AT thakareprashantv simplemethodforisolationofgenomicdnafromfreshanddryleavesofterminaliaarjunaroxbwightandarnot AT chaudhariuddhavs simplemethodforisolationofgenomicdnafromfreshanddryleavesofterminaliaarjunaroxbwightandarnot AT gawandeprashanta simplemethodforisolationofgenomicdnafromfreshanddryleavesofterminaliaarjunaroxbwightandarnot
_version_	1718441772694110208

A simple method for isolation of genomic DNA from fresh and dry leaves of Terminalia arjuna (Roxb.) Wight and Arnot

Ejemplares similares