Isolation of simple sequence repeats from groundnut

SSRs have proved to be the most powerful tool for variety identification in groundnut of similar origin, and have much potential in genetic and breeding studies. To facilitate SSR discovery in groundnut, we proposed a highly simplified SSR isolation protocol based on multiple enzyme digestion/ligati...

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Autores principales:	Tang Wang,Chuan, Dao Yang,Xin, Xu Chen,Dian, Lin Yu,Shan, Zhen Liu,Guang, Yi Tang,Yue, Zhi Xu,Jian
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2007
Materias:	groundnut isolation simple sequence repeat
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000300015
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spelling	oai:scielo:S0717-345820070003000152007-11-05Isolation of simple sequence repeats from groundnutTang Wang,ChuanDao Yang,XinXu Chen,DianLin Yu,ShanZhen Liu,GuangYi Tang,YueZhi Xu,Jian groundnut isolation simple sequence repeat SSRs have proved to be the most powerful tool for variety identification in groundnut of similar origin, and have much potential in genetic and breeding studies. To facilitate SSR discovery in groundnut, we proposed a highly simplified SSR isolation protocol based on multiple enzyme digestion/ligation, mixed biotin-labeled probes and streptavidin coated magnetic beads hybridization capture strategy. Of the 272 colonies randomly picked for sequencing, 119 were found to have unique SSR insertsinfo:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.10 n.3 20072007-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000300015en10.4067/S0717-34582007000300015
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	groundnut isolation simple sequence repeat
spellingShingle	groundnut isolation simple sequence repeat Tang Wang,Chuan Dao Yang,Xin Xu Chen,Dian Lin Yu,Shan Zhen Liu,Guang Yi Tang,Yue Zhi Xu,Jian Isolation of simple sequence repeats from groundnut
description	SSRs have proved to be the most powerful tool for variety identification in groundnut of similar origin, and have much potential in genetic and breeding studies. To facilitate SSR discovery in groundnut, we proposed a highly simplified SSR isolation protocol based on multiple enzyme digestion/ligation, mixed biotin-labeled probes and streptavidin coated magnetic beads hybridization capture strategy. Of the 272 colonies randomly picked for sequencing, 119 were found to have unique SSR inserts
author	Tang Wang,Chuan Dao Yang,Xin Xu Chen,Dian Lin Yu,Shan Zhen Liu,Guang Yi Tang,Yue Zhi Xu,Jian
author_facet	Tang Wang,Chuan Dao Yang,Xin Xu Chen,Dian Lin Yu,Shan Zhen Liu,Guang Yi Tang,Yue Zhi Xu,Jian
author_sort	Tang Wang,Chuan
title	Isolation of simple sequence repeats from groundnut
title_short	Isolation of simple sequence repeats from groundnut
title_full	Isolation of simple sequence repeats from groundnut
title_fullStr	Isolation of simple sequence repeats from groundnut
title_full_unstemmed	Isolation of simple sequence repeats from groundnut
title_sort	isolation of simple sequence repeats from groundnut
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2007
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000300015
work_keys_str_mv	AT tangwangchuan isolationofsimplesequencerepeatsfromgroundnut AT daoyangxin isolationofsimplesequencerepeatsfromgroundnut AT xuchendian isolationofsimplesequencerepeatsfromgroundnut AT linyushan isolationofsimplesequencerepeatsfromgroundnut AT zhenliuguang isolationofsimplesequencerepeatsfromgroundnut AT yitangyue isolationofsimplesequencerepeatsfromgroundnut AT zhixujian isolationofsimplesequencerepeatsfromgroundnut
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Isolation of simple sequence repeats from groundnut

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