Heterologous expression, purification and refolding of an anti-listerial peptide produced by Pediococcus acidilactici K7
The fusion protein, 6XHis-Xpress-PedA was constructed and expressed in Escherichia coli BL21 (DE3). The presence of a 12.8 kDa recombinant protein, localized in inclusion bodies (IBs) at high concentration, was confirmed by SDS-PAGE analysis and by western blotting using anti-His antibody. The rec-p...
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| Autores principales: | , |
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| Lenguaje: | English |
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Pontificia Universidad Católica de Valparaíso
2007
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| Acceso en línea: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000400009 |
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