Inter simple sequence repeats separate efficiently hemp from marijuana (Cannabis sativa L.)

Cannabis sativa L. is a multiple-use plant that provides raw material for the production of seed oil, natural fiber for textiles, automotive and pulp industries. It has also been used in insulating boards, ropes, varnishes, animal feed, and as medicinal agents. Cannabis has potential to be used for...

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Autores principales:	Hakki,Erdogan E, Kayis,Seyit A, Pinarkara,Emine, Sag,Ayla
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2007
Materias:	cluster analysis hemp ISSR marijuana principle coordinate analysis
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000400010
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spelling	oai:scielo:S0717-345820070004000102009-01-19Inter simple sequence repeats separate efficiently hemp from marijuana (Cannabis sativa L.)Hakki,Erdogan EKayis,Seyit APinarkara,EmineSag,Ayla cluster analysis hemp ISSR marijuana principle coordinate analysis Cannabis sativa L. is a multiple-use plant that provides raw material for the production of seed oil, natural fiber for textiles, automotive and pulp industries. It has also been used in insulating boards, ropes, varnishes, animal feed, and as medicinal agents. Cannabis has potential to be used for phytoremediation: however, its cultivation is strictly controlled due to its psychoactive nature and usage in producing drugs such as marijuana, and hashish. In this study, psychoactive type Cannabis samples, which were seized from 23 different locations of Turkey, and nine hemp type Cannabis accessions, as well as an unknown accession were used. Our interest was to identify the genetic relatedness of the seized samples and to separate drug and hemp type plants. Inter Simple Sequence Repeats (ISSRs) were employed for analysis based on single plant material (SET1) and bulked samples of them (SET2). Data was analysed via cluster analysis and principal coordinate analysis (PCoA). PCoA analyses, by using SET1 and SET2, were able to efficiently discriminate the seized samples from the fiber type accessions. However, separation of the plants was not clear via unweighted pair-group method using arithmetic average (UPGMA) dendogram in SET1, while they were clearly separated in SET2. Hemp type accessions showed high levels of variation compared to drug type Cannabis both in SET1 and SET2.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.10 n.4 20072007-10-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000400010en10.4067/S0717-34582007000400010
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	cluster analysis hemp ISSR marijuana principle coordinate analysis
spellingShingle	cluster analysis hemp ISSR marijuana principle coordinate analysis Hakki,Erdogan E Kayis,Seyit A Pinarkara,Emine Sag,Ayla Inter simple sequence repeats separate efficiently hemp from marijuana (Cannabis sativa L.)
description	Cannabis sativa L. is a multiple-use plant that provides raw material for the production of seed oil, natural fiber for textiles, automotive and pulp industries. It has also been used in insulating boards, ropes, varnishes, animal feed, and as medicinal agents. Cannabis has potential to be used for phytoremediation: however, its cultivation is strictly controlled due to its psychoactive nature and usage in producing drugs such as marijuana, and hashish. In this study, psychoactive type Cannabis samples, which were seized from 23 different locations of Turkey, and nine hemp type Cannabis accessions, as well as an unknown accession were used. Our interest was to identify the genetic relatedness of the seized samples and to separate drug and hemp type plants. Inter Simple Sequence Repeats (ISSRs) were employed for analysis based on single plant material (SET1) and bulked samples of them (SET2). Data was analysed via cluster analysis and principal coordinate analysis (PCoA). PCoA analyses, by using SET1 and SET2, were able to efficiently discriminate the seized samples from the fiber type accessions. However, separation of the plants was not clear via unweighted pair-group method using arithmetic average (UPGMA) dendogram in SET1, while they were clearly separated in SET2. Hemp type accessions showed high levels of variation compared to drug type Cannabis both in SET1 and SET2.
author	Hakki,Erdogan E Kayis,Seyit A Pinarkara,Emine Sag,Ayla
author_facet	Hakki,Erdogan E Kayis,Seyit A Pinarkara,Emine Sag,Ayla
author_sort	Hakki,Erdogan E
title	Inter simple sequence repeats separate efficiently hemp from marijuana (Cannabis sativa L.)
title_short	Inter simple sequence repeats separate efficiently hemp from marijuana (Cannabis sativa L.)
title_full	Inter simple sequence repeats separate efficiently hemp from marijuana (Cannabis sativa L.)
title_fullStr	Inter simple sequence repeats separate efficiently hemp from marijuana (Cannabis sativa L.)
title_full_unstemmed	Inter simple sequence repeats separate efficiently hemp from marijuana (Cannabis sativa L.)
title_sort	inter simple sequence repeats separate efficiently hemp from marijuana (cannabis sativa l.)
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2007
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000400010
work_keys_str_mv	AT hakkierdogane intersimplesequencerepeatsseparateefficientlyhempfrommarijuanacannabissatival AT kayisseyita intersimplesequencerepeatsseparateefficientlyhempfrommarijuanacannabissatival AT pinarkaraemine intersimplesequencerepeatsseparateefficientlyhempfrommarijuanacannabissatival AT sagayla intersimplesequencerepeatsseparateefficientlyhempfrommarijuanacannabissatival
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Inter simple sequence repeats separate efficiently hemp from marijuana (Cannabis sativa L.)

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