Micropropagation of Lilium ledebourii (Baker) Boiss as affected by plant growth regulator, sucrose concentration, harvesting season and cold treatments

A protocol for the micropropagation in different harvesting time of Lilium ledebourii (Baker) Boiss, an endangered rare species endemic to Iran has been developed. In vitro scale culture of this species, using bulbs from three harvesting seasons (spring, summer and winter), was attempted. Among the...

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Autores principales:	Azadi,Pejman, Khosh-Khui,Morteza
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2007
Materias:	bulblet cold treatment conservation harvesting season Lilium ledebourii tissue culture
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000400011
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spelling	oai:scielo:S0717-345820070004000112009-01-19Micropropagation of Lilium ledebourii (Baker) Boiss as affected by plant growth regulator, sucrose concentration, harvesting season and cold treatmentsAzadi,PejmanKhosh-Khui,Morteza bulblet cold treatment conservation harvesting season Lilium ledebourii tissue culture A protocol for the micropropagation in different harvesting time of Lilium ledebourii (Baker) Boiss, an endangered rare species endemic to Iran has been developed. In vitro scale culture of this species, using bulbs from three harvesting seasons (spring, summer and winter), was attempted. Among the various treatments tested, the Murashige and Skoog (MS) medium supplemented with 0.1 mg l-1 naphthaleneacetic acid (NAA) + 0.1 mg l-1 benzyladenin (BA) and 6% sucrose in all harvesting seasons proved to be superior to others. The best results for fresh weight of bulblets, rooting parameters and the survival rate after transplantation to greenhouse were obtained from early winter-harvested bulbs. Summer-harvested bulbs had the highest number of bulblets per explant. The bulblets at the end of the culture period were given cold treatment at 4ºC for 2-8 weeks at a 2-weeks interval and then transplanted to a potting mixture of sand, leaf mold and peat moss (1:1:1 v/v). The best emergence rate (90%) was achieved at 8 weeks cold treatment for winter harvested bulbs.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.10 n.4 20072007-10-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000400011en10.4067/S0717-34582007000400011
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	bulblet cold treatment conservation harvesting season Lilium ledebourii tissue culture
spellingShingle	bulblet cold treatment conservation harvesting season Lilium ledebourii tissue culture Azadi,Pejman Khosh-Khui,Morteza Micropropagation of Lilium ledebourii (Baker) Boiss as affected by plant growth regulator, sucrose concentration, harvesting season and cold treatments
description	A protocol for the micropropagation in different harvesting time of Lilium ledebourii (Baker) Boiss, an endangered rare species endemic to Iran has been developed. In vitro scale culture of this species, using bulbs from three harvesting seasons (spring, summer and winter), was attempted. Among the various treatments tested, the Murashige and Skoog (MS) medium supplemented with 0.1 mg l-1 naphthaleneacetic acid (NAA) + 0.1 mg l-1 benzyladenin (BA) and 6% sucrose in all harvesting seasons proved to be superior to others. The best results for fresh weight of bulblets, rooting parameters and the survival rate after transplantation to greenhouse were obtained from early winter-harvested bulbs. Summer-harvested bulbs had the highest number of bulblets per explant. The bulblets at the end of the culture period were given cold treatment at 4ºC for 2-8 weeks at a 2-weeks interval and then transplanted to a potting mixture of sand, leaf mold and peat moss (1:1:1 v/v). The best emergence rate (90%) was achieved at 8 weeks cold treatment for winter harvested bulbs.
author	Azadi,Pejman Khosh-Khui,Morteza
author_facet	Azadi,Pejman Khosh-Khui,Morteza
author_sort	Azadi,Pejman
title	Micropropagation of Lilium ledebourii (Baker) Boiss as affected by plant growth regulator, sucrose concentration, harvesting season and cold treatments
title_short	Micropropagation of Lilium ledebourii (Baker) Boiss as affected by plant growth regulator, sucrose concentration, harvesting season and cold treatments
title_full	Micropropagation of Lilium ledebourii (Baker) Boiss as affected by plant growth regulator, sucrose concentration, harvesting season and cold treatments
title_fullStr	Micropropagation of Lilium ledebourii (Baker) Boiss as affected by plant growth regulator, sucrose concentration, harvesting season and cold treatments
title_full_unstemmed	Micropropagation of Lilium ledebourii (Baker) Boiss as affected by plant growth regulator, sucrose concentration, harvesting season and cold treatments
title_sort	micropropagation of lilium ledebourii (baker) boiss as affected by plant growth regulator, sucrose concentration, harvesting season and cold treatments
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2007
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000400011
work_keys_str_mv	AT azadipejman micropropagationofliliumledebouriibakerboissasaffectedbyplantgrowthregulatorsucroseconcentrationharvestingseasonandcoldtreatments AT khoshkhuimorteza micropropagationofliliumledebouriibakerboissasaffectedbyplantgrowthregulatorsucroseconcentrationharvestingseasonandcoldtreatments
_version_	1718441775883878400

Micropropagation of Lilium ledebourii (Baker) Boiss as affected by plant growth regulator, sucrose concentration, harvesting season and cold treatments

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