Optimization of medium composition for transglutaminase production by a Brazilian soil Streptomyces sp.

Finding a new microbial source of transglutaminase (MTGase) and the study of the medium composition for MTGase production were the goals of this work. A total of 200 actinomycete-like strains were isolated from Brazilian soil samples and two of them named T10b and P20 were selected based on their ab...

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Autores principales:	Macedo,Juliana Alves, Sette,Lara Durães, Sato,Hélia Harumi
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2007
Materias:	central composite design fractional factorial design medium optimization microbial transglutaminase Streptomyces sp. P20
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000400014
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spelling	oai:scielo:S0717-345820070004000142009-01-19Optimization of medium composition for transglutaminase production by a Brazilian soil Streptomyces sp.Macedo,Juliana AlvesSette,Lara DurãesSato,Hélia Harumi central composite design fractional factorial design medium optimization microbial transglutaminase Streptomyces sp. P20 Finding a new microbial source of transglutaminase (MTGase) and the study of the medium composition for MTGase production were the goals of this work. A total of 200 actinomycete-like strains were isolated from Brazilian soil samples and two of them named T10b and P20 were selected based on their ability to produce 0.15 U.mL-1 and 0.25 U.mL-1 of MTGase, respectively. Strain P20 was chosen to continue the study and was identified as Streptomyces sp. In order to optimize the MTGase activity, modifications of the usual media composition described for enzyme production were tested. The strategy adopted was: (1) screening experiment for the best carbon and nitrogen sources; (2) fractional factorial design (FFD) to elucidate the key ingredients in the media (the results indicated that the soybean flour, peptone, KH2PO4 and MgSO4.7H2O had a significant effect on MTGase) production and (3) central composite design (CCD) to optimize the concentration of the key components. The experimental results were fitted to a second-order polynomial model at the 95% level of significance (P < 0.05). Under the proposed optimized conditions, the model predicted a MTGase activity of 1.37 U.mL-1, very closely matching the experimental activity of 1.4 U.mL-1.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.10 n.4 20072007-10-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000400014en10.4067/S0717-34582007000400014
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	central composite design fractional factorial design medium optimization microbial transglutaminase Streptomyces sp. P20
spellingShingle	central composite design fractional factorial design medium optimization microbial transglutaminase Streptomyces sp. P20 Macedo,Juliana Alves Sette,Lara Durães Sato,Hélia Harumi Optimization of medium composition for transglutaminase production by a Brazilian soil Streptomyces sp.
description	Finding a new microbial source of transglutaminase (MTGase) and the study of the medium composition for MTGase production were the goals of this work. A total of 200 actinomycete-like strains were isolated from Brazilian soil samples and two of them named T10b and P20 were selected based on their ability to produce 0.15 U.mL-1 and 0.25 U.mL-1 of MTGase, respectively. Strain P20 was chosen to continue the study and was identified as Streptomyces sp. In order to optimize the MTGase activity, modifications of the usual media composition described for enzyme production were tested. The strategy adopted was: (1) screening experiment for the best carbon and nitrogen sources; (2) fractional factorial design (FFD) to elucidate the key ingredients in the media (the results indicated that the soybean flour, peptone, KH2PO4 and MgSO4.7H2O had a significant effect on MTGase) production and (3) central composite design (CCD) to optimize the concentration of the key components. The experimental results were fitted to a second-order polynomial model at the 95% level of significance (P < 0.05). Under the proposed optimized conditions, the model predicted a MTGase activity of 1.37 U.mL-1, very closely matching the experimental activity of 1.4 U.mL-1.
author	Macedo,Juliana Alves Sette,Lara Durães Sato,Hélia Harumi
author_facet	Macedo,Juliana Alves Sette,Lara Durães Sato,Hélia Harumi
author_sort	Macedo,Juliana Alves
title	Optimization of medium composition for transglutaminase production by a Brazilian soil Streptomyces sp.
title_short	Optimization of medium composition for transglutaminase production by a Brazilian soil Streptomyces sp.
title_full	Optimization of medium composition for transglutaminase production by a Brazilian soil Streptomyces sp.
title_fullStr	Optimization of medium composition for transglutaminase production by a Brazilian soil Streptomyces sp.
title_full_unstemmed	Optimization of medium composition for transglutaminase production by a Brazilian soil Streptomyces sp.
title_sort	optimization of medium composition for transglutaminase production by a brazilian soil streptomyces sp.
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2007
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000400014
work_keys_str_mv	AT macedojulianaalves optimizationofmediumcompositionfortransglutaminaseproductionbyabraziliansoilstreptomycessp AT settelaraduraes optimizationofmediumcompositionfortransglutaminaseproductionbyabraziliansoilstreptomycessp AT satoheliaharumi optimizationofmediumcompositionfortransglutaminaseproductionbyabraziliansoilstreptomycessp
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Optimization of medium composition for transglutaminase production by a Brazilian soil Streptomyces sp.

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