Phage-resistance of Salmonella enterica serovar Enteritidis and pathogenesis in Caenorhabditis elegans is mediated by the lipopolysaccharide

Phage therapy has been used in the past as an alternative therapy against bacterial pathogens. However, phage-resistant bacterial strains can emerge. Some studies show that these phage-resistant strains are avirulent. In this study, we report that phage-resistant strains of Salmonella enterica serov...

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Autores principales:	Santander,Javier, Robeson,James
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2007
Materias:	animal model bacteriophage prophylaxis Caenorhabditis elegans phage resistance Salmonella
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000400015
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spelling	oai:scielo:S0717-345820070004000152009-01-19Phage-resistance of Salmonella enterica serovar Enteritidis and pathogenesis in Caenorhabditis elegans is mediated by the lipopolysaccharideSantander,JavierRobeson,James animal model bacteriophage prophylaxis Caenorhabditis elegans phage resistance Salmonella Phage therapy has been used in the past as an alternative therapy against bacterial pathogens. However, phage-resistant bacterial strains can emerge. Some studies show that these phage-resistant strains are avirulent. In this study, we report that phage-resistant strains of Salmonella enterica serovar Enteritidis (hereafter S. Enteritidis) were avirulent in the Caenorhabditis elegans animal model. We isolated phage-resistant strains of S. Enteritidis ATCC 13076 by using three lytic phages (f2αSE, f3αSE and f18αSE). In these mutants, we explored different virulence factors like lipopolysaccharide (LPS), virulence plasmid (Pla), motility and type I fimbriae, all of which may have effects on virulence and could furthermore be related to phage resistance. The phage-resistant strains of S. Enteritidis showed loss of O-Polysaccharide (O-PS) and auto-agglutination, present a rough phenotype and consequently they are avirulent in the C. elegans animal model. We speculate that the O-PS is necessary for phage attachment to the S. Enteritidis cell surface.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.10 n.4 20072007-10-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000400015en10.4067/S0717-34582007000400015
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	animal model bacteriophage prophylaxis Caenorhabditis elegans phage resistance Salmonella
spellingShingle	animal model bacteriophage prophylaxis Caenorhabditis elegans phage resistance Salmonella Santander,Javier Robeson,James Phage-resistance of Salmonella enterica serovar Enteritidis and pathogenesis in Caenorhabditis elegans is mediated by the lipopolysaccharide
description	Phage therapy has been used in the past as an alternative therapy against bacterial pathogens. However, phage-resistant bacterial strains can emerge. Some studies show that these phage-resistant strains are avirulent. In this study, we report that phage-resistant strains of Salmonella enterica serovar Enteritidis (hereafter S. Enteritidis) were avirulent in the Caenorhabditis elegans animal model. We isolated phage-resistant strains of S. Enteritidis ATCC 13076 by using three lytic phages (f2αSE, f3αSE and f18αSE). In these mutants, we explored different virulence factors like lipopolysaccharide (LPS), virulence plasmid (Pla), motility and type I fimbriae, all of which may have effects on virulence and could furthermore be related to phage resistance. The phage-resistant strains of S. Enteritidis showed loss of O-Polysaccharide (O-PS) and auto-agglutination, present a rough phenotype and consequently they are avirulent in the C. elegans animal model. We speculate that the O-PS is necessary for phage attachment to the S. Enteritidis cell surface.
author	Santander,Javier Robeson,James
author_facet	Santander,Javier Robeson,James
author_sort	Santander,Javier
title	Phage-resistance of Salmonella enterica serovar Enteritidis and pathogenesis in Caenorhabditis elegans is mediated by the lipopolysaccharide
title_short	Phage-resistance of Salmonella enterica serovar Enteritidis and pathogenesis in Caenorhabditis elegans is mediated by the lipopolysaccharide
title_full	Phage-resistance of Salmonella enterica serovar Enteritidis and pathogenesis in Caenorhabditis elegans is mediated by the lipopolysaccharide
title_fullStr	Phage-resistance of Salmonella enterica serovar Enteritidis and pathogenesis in Caenorhabditis elegans is mediated by the lipopolysaccharide
title_full_unstemmed	Phage-resistance of Salmonella enterica serovar Enteritidis and pathogenesis in Caenorhabditis elegans is mediated by the lipopolysaccharide
title_sort	phage-resistance of salmonella enterica serovar enteritidis and pathogenesis in caenorhabditis elegans is mediated by the lipopolysaccharide
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2007
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000400015
work_keys_str_mv	AT santanderjavier phageresistanceofsalmonellaentericaserovarenteritidisandpathogenesisincaenorhabditiselegansismediatedbythelipopolysaccharide AT robesonjames phageresistanceofsalmonellaentericaserovarenteritidisandpathogenesisincaenorhabditiselegansismediatedbythelipopolysaccharide
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Phage-resistance of Salmonella enterica serovar Enteritidis and pathogenesis in Caenorhabditis elegans is mediated by the lipopolysaccharide

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