Diversity of bacterial communities in acid mine drainage from the Shen-bu copper mine, Gansu province, China

This study presents bacterial population analyses of microbial communities inhabiting three sites of acid mine drainage (AMD) in the Shen-bu copper mine, Gansu Province, China. These sites were located next to acid-leached chalcopyrite slagheaps that had been abandoned since 1995. The pH values of t...

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Autores principales:	Yang,Yu, Shi,Wuyang, Wan,Minxi, Zhang,Yanfei, Zou,Lihong, Huang,Jufang, Qiu,Guanzhou, Liu,Xueduan
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2008
Materias:	acid mine drainage amplified ribosomal DNA restriction analysis microbial communities
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000100001
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spelling	oai:scielo:S0717-345820080001000012008-10-28Diversity of bacterial communities in acid mine drainage from the Shen-bu copper mine, Gansu province, ChinaYang,YuShi,WuyangWan,MinxiZhang,YanfeiZou,LihongHuang,JufangQiu,GuanzhouLiu,Xueduan acid mine drainage amplified ribosomal DNA restriction analysis microbial communities This study presents bacterial population analyses of microbial communities inhabiting three sites of acid mine drainage (AMD) in the Shen-bu copper mine, Gansu Province, China. These sites were located next to acid-leached chalcopyrite slagheaps that had been abandoned since 1995. The pH values of these samples with high concentrations of metals ranged from 2.0 to 3.5. Amplified ribosomal DNA restriction analysis (ARDRA) was used to characterize the bacterial population by amplifying the 16S rRNA gene of microorganisms. A total of 39 operational taxonomic units (OTUs) were obtained from the three samples and sequenced from 384 clones. Sequence data and phylogenetic analyses showed that two dominant clones (JYC-1B, JYC-1D) in sample JYC-1 represented 69.5% of the total clones affiliated with Acidithiobacillus ferrooxidans (γ-Proteobacteria), and the most dominant clones of JYC-2 and JYC-3 were affiliated with Caulobacter crescentus (α-Protebacteria). At the level of bacterial divisions, differences in the relative incidence of particular phylogenetic groups among the three samples and discrepancies in physicochemical characteristics suggested that the physico-chemical characteristics had an influence on phylogenetic diversity. Furthermore, the relationships between the discrepancies of physicochemical characteristics and the diversity of the bacteria communities in the three samples suggested that the biogeochemical properties, pH and concentration of soluble metal, could be key factors in controlling the structure of the bacterial populationinfo:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.11 n.1 20082008-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000100001en10.4067/S0717-34582008000100001
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	acid mine drainage amplified ribosomal DNA restriction analysis microbial communities
spellingShingle	acid mine drainage amplified ribosomal DNA restriction analysis microbial communities Yang,Yu Shi,Wuyang Wan,Minxi Zhang,Yanfei Zou,Lihong Huang,Jufang Qiu,Guanzhou Liu,Xueduan Diversity of bacterial communities in acid mine drainage from the Shen-bu copper mine, Gansu province, China
description	This study presents bacterial population analyses of microbial communities inhabiting three sites of acid mine drainage (AMD) in the Shen-bu copper mine, Gansu Province, China. These sites were located next to acid-leached chalcopyrite slagheaps that had been abandoned since 1995. The pH values of these samples with high concentrations of metals ranged from 2.0 to 3.5. Amplified ribosomal DNA restriction analysis (ARDRA) was used to characterize the bacterial population by amplifying the 16S rRNA gene of microorganisms. A total of 39 operational taxonomic units (OTUs) were obtained from the three samples and sequenced from 384 clones. Sequence data and phylogenetic analyses showed that two dominant clones (JYC-1B, JYC-1D) in sample JYC-1 represented 69.5% of the total clones affiliated with Acidithiobacillus ferrooxidans (γ-Proteobacteria), and the most dominant clones of JYC-2 and JYC-3 were affiliated with Caulobacter crescentus (α-Protebacteria). At the level of bacterial divisions, differences in the relative incidence of particular phylogenetic groups among the three samples and discrepancies in physicochemical characteristics suggested that the physico-chemical characteristics had an influence on phylogenetic diversity. Furthermore, the relationships between the discrepancies of physicochemical characteristics and the diversity of the bacteria communities in the three samples suggested that the biogeochemical properties, pH and concentration of soluble metal, could be key factors in controlling the structure of the bacterial population
author	Yang,Yu Shi,Wuyang Wan,Minxi Zhang,Yanfei Zou,Lihong Huang,Jufang Qiu,Guanzhou Liu,Xueduan
author_facet	Yang,Yu Shi,Wuyang Wan,Minxi Zhang,Yanfei Zou,Lihong Huang,Jufang Qiu,Guanzhou Liu,Xueduan
author_sort	Yang,Yu
title	Diversity of bacterial communities in acid mine drainage from the Shen-bu copper mine, Gansu province, China
title_short	Diversity of bacterial communities in acid mine drainage from the Shen-bu copper mine, Gansu province, China
title_full	Diversity of bacterial communities in acid mine drainage from the Shen-bu copper mine, Gansu province, China
title_fullStr	Diversity of bacterial communities in acid mine drainage from the Shen-bu copper mine, Gansu province, China
title_full_unstemmed	Diversity of bacterial communities in acid mine drainage from the Shen-bu copper mine, Gansu province, China
title_sort	diversity of bacterial communities in acid mine drainage from the shen-bu copper mine, gansu province, china
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2008
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000100001
work_keys_str_mv	AT yangyu diversityofbacterialcommunitiesinacidminedrainagefromtheshenbucopperminegansuprovincechina AT shiwuyang diversityofbacterialcommunitiesinacidminedrainagefromtheshenbucopperminegansuprovincechina AT wanminxi diversityofbacterialcommunitiesinacidminedrainagefromtheshenbucopperminegansuprovincechina AT zhangyanfei diversityofbacterialcommunitiesinacidminedrainagefromtheshenbucopperminegansuprovincechina AT zoulihong diversityofbacterialcommunitiesinacidminedrainagefromtheshenbucopperminegansuprovincechina AT huangjufang diversityofbacterialcommunitiesinacidminedrainagefromtheshenbucopperminegansuprovincechina AT qiuguanzhou diversityofbacterialcommunitiesinacidminedrainagefromtheshenbucopperminegansuprovincechina AT liuxueduan diversityofbacterialcommunitiesinacidminedrainagefromtheshenbucopperminegansuprovincechina
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Diversity of bacterial communities in acid mine drainage from the Shen-bu copper mine, Gansu province, China

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