Heterozygosity following half-sib recurrent selection in popcorn using isoenzyme markers

Isozyme biochemical marker may be useful tool for genomic analysis of maize populations undergoing recurrent selection. Thus, isozymes markers was utilized for assess the changes in the genetic variability and distance in a Brazilian composite population of popcorn following four cycles of recurrent...

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Autores principales: Pereira,Liz Kazmirczak, Scapim,Carlos Alberto, Mangolin,Claudete Aparecida, Machado,Maria de Fátima Pires da Silva, Pacheco,Cleso Antônio Patto, Mora,Freddy
Lenguaje:English
Publicado: Pontificia Universidad Católica de Valparaíso 2008
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Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000100004
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spelling oai:scielo:S0717-345820080001000042008-10-28Heterozygosity following half-sib recurrent selection in popcorn using isoenzyme markersPereira,Liz KazmirczakScapim,Carlos AlbertoMangolin,Claudete AparecidaMachado,Maria de Fátima Pires da SilvaPacheco,Cleso Antônio PattoMora,Freddy allozymes frequency genetic distance selection cycles Zea mays Isozyme biochemical marker may be useful tool for genomic analysis of maize populations undergoing recurrent selection. Thus, isozymes markers was utilized for assess the changes in the genetic variability and distance in a Brazilian composite population of popcorn following four cycles of recurrent selection for yield. One hundred and ninety-six half-sib families were evaluated from each cycle and the ten highest-yielding families (5.2%) were recombined to produce the next cycle. Isozyme analysis considered 80 seedlings per cycle. Simple linear regression equations were estimated among the allele frequencies in each locus in function of the selection cycles, the genetic distances among the cycles and the average heterozygosity per locus for each cycle. Regression analysis did not reveal any common trend for changes in allele frequencies presumably due to selection. The estimates of the number of polymorphic locus, of the mean of allele per locus and the mean heterozygosity did not reveal any reduction in variability. It was concluded that four selection cycles did not cause relevant changes in the variability or genetic distance among the selection cycles of CMS-43 popcorn population. Isozymes markers analysis showed that the number of recombined half-sib families in recurrent selection was suitable.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.11 n.1 20082008-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000100004en10.4067/S0717-34582008000100004
institution Scielo Chile
collection Scielo Chile
language English
topic allozymes frequency
genetic distance
selection cycles
Zea mays
spellingShingle allozymes frequency
genetic distance
selection cycles
Zea mays
Pereira,Liz Kazmirczak
Scapim,Carlos Alberto
Mangolin,Claudete Aparecida
Machado,Maria de Fátima Pires da Silva
Pacheco,Cleso Antônio Patto
Mora,Freddy
Heterozygosity following half-sib recurrent selection in popcorn using isoenzyme markers
description Isozyme biochemical marker may be useful tool for genomic analysis of maize populations undergoing recurrent selection. Thus, isozymes markers was utilized for assess the changes in the genetic variability and distance in a Brazilian composite population of popcorn following four cycles of recurrent selection for yield. One hundred and ninety-six half-sib families were evaluated from each cycle and the ten highest-yielding families (5.2%) were recombined to produce the next cycle. Isozyme analysis considered 80 seedlings per cycle. Simple linear regression equations were estimated among the allele frequencies in each locus in function of the selection cycles, the genetic distances among the cycles and the average heterozygosity per locus for each cycle. Regression analysis did not reveal any common trend for changes in allele frequencies presumably due to selection. The estimates of the number of polymorphic locus, of the mean of allele per locus and the mean heterozygosity did not reveal any reduction in variability. It was concluded that four selection cycles did not cause relevant changes in the variability or genetic distance among the selection cycles of CMS-43 popcorn population. Isozymes markers analysis showed that the number of recombined half-sib families in recurrent selection was suitable.
author Pereira,Liz Kazmirczak
Scapim,Carlos Alberto
Mangolin,Claudete Aparecida
Machado,Maria de Fátima Pires da Silva
Pacheco,Cleso Antônio Patto
Mora,Freddy
author_facet Pereira,Liz Kazmirczak
Scapim,Carlos Alberto
Mangolin,Claudete Aparecida
Machado,Maria de Fátima Pires da Silva
Pacheco,Cleso Antônio Patto
Mora,Freddy
author_sort Pereira,Liz Kazmirczak
title Heterozygosity following half-sib recurrent selection in popcorn using isoenzyme markers
title_short Heterozygosity following half-sib recurrent selection in popcorn using isoenzyme markers
title_full Heterozygosity following half-sib recurrent selection in popcorn using isoenzyme markers
title_fullStr Heterozygosity following half-sib recurrent selection in popcorn using isoenzyme markers
title_full_unstemmed Heterozygosity following half-sib recurrent selection in popcorn using isoenzyme markers
title_sort heterozygosity following half-sib recurrent selection in popcorn using isoenzyme markers
publisher Pontificia Universidad Católica de Valparaíso
publishDate 2008
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000100004
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