Heterozygosity following half-sib recurrent selection in popcorn using isoenzyme markers
Isozyme biochemical marker may be useful tool for genomic analysis of maize populations undergoing recurrent selection. Thus, isozymes markers was utilized for assess the changes in the genetic variability and distance in a Brazilian composite population of popcorn following four cycles of recurrent...
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Pontificia Universidad Católica de Valparaíso
2008
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oai:scielo:S0717-345820080001000042008-10-28Heterozygosity following half-sib recurrent selection in popcorn using isoenzyme markersPereira,Liz KazmirczakScapim,Carlos AlbertoMangolin,Claudete AparecidaMachado,Maria de Fátima Pires da SilvaPacheco,Cleso Antônio PattoMora,Freddy allozymes frequency genetic distance selection cycles Zea mays Isozyme biochemical marker may be useful tool for genomic analysis of maize populations undergoing recurrent selection. Thus, isozymes markers was utilized for assess the changes in the genetic variability and distance in a Brazilian composite population of popcorn following four cycles of recurrent selection for yield. One hundred and ninety-six half-sib families were evaluated from each cycle and the ten highest-yielding families (5.2%) were recombined to produce the next cycle. Isozyme analysis considered 80 seedlings per cycle. Simple linear regression equations were estimated among the allele frequencies in each locus in function of the selection cycles, the genetic distances among the cycles and the average heterozygosity per locus for each cycle. Regression analysis did not reveal any common trend for changes in allele frequencies presumably due to selection. The estimates of the number of polymorphic locus, of the mean of allele per locus and the mean heterozygosity did not reveal any reduction in variability. It was concluded that four selection cycles did not cause relevant changes in the variability or genetic distance among the selection cycles of CMS-43 popcorn population. Isozymes markers analysis showed that the number of recombined half-sib families in recurrent selection was suitable.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.11 n.1 20082008-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000100004en10.4067/S0717-34582008000100004 |
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Scielo Chile |
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English |
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allozymes frequency genetic distance selection cycles Zea mays |
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allozymes frequency genetic distance selection cycles Zea mays Pereira,Liz Kazmirczak Scapim,Carlos Alberto Mangolin,Claudete Aparecida Machado,Maria de Fátima Pires da Silva Pacheco,Cleso Antônio Patto Mora,Freddy Heterozygosity following half-sib recurrent selection in popcorn using isoenzyme markers |
description |
Isozyme biochemical marker may be useful tool for genomic analysis of maize populations undergoing recurrent selection. Thus, isozymes markers was utilized for assess the changes in the genetic variability and distance in a Brazilian composite population of popcorn following four cycles of recurrent selection for yield. One hundred and ninety-six half-sib families were evaluated from each cycle and the ten highest-yielding families (5.2%) were recombined to produce the next cycle. Isozyme analysis considered 80 seedlings per cycle. Simple linear regression equations were estimated among the allele frequencies in each locus in function of the selection cycles, the genetic distances among the cycles and the average heterozygosity per locus for each cycle. Regression analysis did not reveal any common trend for changes in allele frequencies presumably due to selection. The estimates of the number of polymorphic locus, of the mean of allele per locus and the mean heterozygosity did not reveal any reduction in variability. It was concluded that four selection cycles did not cause relevant changes in the variability or genetic distance among the selection cycles of CMS-43 popcorn population. Isozymes markers analysis showed that the number of recombined half-sib families in recurrent selection was suitable. |
author |
Pereira,Liz Kazmirczak Scapim,Carlos Alberto Mangolin,Claudete Aparecida Machado,Maria de Fátima Pires da Silva Pacheco,Cleso Antônio Patto Mora,Freddy |
author_facet |
Pereira,Liz Kazmirczak Scapim,Carlos Alberto Mangolin,Claudete Aparecida Machado,Maria de Fátima Pires da Silva Pacheco,Cleso Antônio Patto Mora,Freddy |
author_sort |
Pereira,Liz Kazmirczak |
title |
Heterozygosity following half-sib recurrent selection in popcorn using isoenzyme markers |
title_short |
Heterozygosity following half-sib recurrent selection in popcorn using isoenzyme markers |
title_full |
Heterozygosity following half-sib recurrent selection in popcorn using isoenzyme markers |
title_fullStr |
Heterozygosity following half-sib recurrent selection in popcorn using isoenzyme markers |
title_full_unstemmed |
Heterozygosity following half-sib recurrent selection in popcorn using isoenzyme markers |
title_sort |
heterozygosity following half-sib recurrent selection in popcorn using isoenzyme markers |
publisher |
Pontificia Universidad Católica de Valparaíso |
publishDate |
2008 |
url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000100004 |
work_keys_str_mv |
AT pereiralizkazmirczak heterozygosityfollowinghalfsibrecurrentselectioninpopcornusingisoenzymemarkers AT scapimcarlosalberto heterozygosityfollowinghalfsibrecurrentselectioninpopcornusingisoenzymemarkers AT mangolinclaudeteaparecida heterozygosityfollowinghalfsibrecurrentselectioninpopcornusingisoenzymemarkers AT machadomariadefatimapiresdasilva heterozygosityfollowinghalfsibrecurrentselectioninpopcornusingisoenzymemarkers AT pachecoclesoantoniopatto heterozygosityfollowinghalfsibrecurrentselectioninpopcornusingisoenzymemarkers AT morafreddy heterozygosityfollowinghalfsibrecurrentselectioninpopcornusingisoenzymemarkers |
_version_ |
1718441778407800832 |