Overexpression of the pineapple fruit bromelain gene (BAA) in transgenic Chinese cabbage (Brassica rapa) results in enhanced resistance to bacterial soft rot

Bromelain is a crude protein extract obtained from pineapple stems, which comprises a variety of proteolytic enzymes. It exhibits potential therapeutic activities against trauma, inflammation, autoimmune diseases and malignant disorders. In this study, we cloned BAA1 (the gene encoding fruit bromela...

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Autores principales:	Jung,Yu-Jin, Choi,Chang-Sun, Park,Jin-Heui, Kang,Hee-Wan, Choi,Je-Eul, Nou,Ill-Sup, Lee,Soon Youl, Kang,Kwon-Kyoo
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2008
Materias:	Brassica rapa fruit bromelain soft rot resistance transgenic plant
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000100007
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spelling	oai:scielo:S0717-345820080001000072008-10-28Overexpression of the pineapple fruit bromelain gene (BAA) in transgenic Chinese cabbage (Brassica rapa) results in enhanced resistance to bacterial soft rotJung,Yu-JinChoi,Chang-SunPark,Jin-HeuiKang,Hee-WanChoi,Je-EulNou,Ill-SupLee,Soon YoulKang,Kwon-Kyoo Brassica rapa fruit bromelain soft rot resistance transgenic plant Bromelain is a crude protein extract obtained from pineapple stems, which comprises a variety of proteolytic enzymes. It exhibits potential therapeutic activities against trauma, inflammation, autoimmune diseases and malignant disorders. In this study, we cloned BAA1 (the gene encoding fruit bromelain) into a plant expression vector that was then used to transform Brassica rapa and overexpress BAA1 under the control of the cauliflower mosaic virus (CaMV) 35S promoter. We demonstrate that constitutive overexpression of BAA1 in B. rapa confers enhanced resistance to the soft rot pathogen Pectobacterium carotovorum ssp. carotovorum. These results suggest that it could be utilized for protecting plants from attack by bacterial pathogensinfo:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.11 n.1 20082008-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000100007en10.4067/S0717-34582008000100007
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Brassica rapa fruit bromelain soft rot resistance transgenic plant
spellingShingle	Brassica rapa fruit bromelain soft rot resistance transgenic plant Jung,Yu-Jin Choi,Chang-Sun Park,Jin-Heui Kang,Hee-Wan Choi,Je-Eul Nou,Ill-Sup Lee,Soon Youl Kang,Kwon-Kyoo Overexpression of the pineapple fruit bromelain gene (BAA) in transgenic Chinese cabbage (Brassica rapa) results in enhanced resistance to bacterial soft rot
description	Bromelain is a crude protein extract obtained from pineapple stems, which comprises a variety of proteolytic enzymes. It exhibits potential therapeutic activities against trauma, inflammation, autoimmune diseases and malignant disorders. In this study, we cloned BAA1 (the gene encoding fruit bromelain) into a plant expression vector that was then used to transform Brassica rapa and overexpress BAA1 under the control of the cauliflower mosaic virus (CaMV) 35S promoter. We demonstrate that constitutive overexpression of BAA1 in B. rapa confers enhanced resistance to the soft rot pathogen Pectobacterium carotovorum ssp. carotovorum. These results suggest that it could be utilized for protecting plants from attack by bacterial pathogens
author	Jung,Yu-Jin Choi,Chang-Sun Park,Jin-Heui Kang,Hee-Wan Choi,Je-Eul Nou,Ill-Sup Lee,Soon Youl Kang,Kwon-Kyoo
author_facet	Jung,Yu-Jin Choi,Chang-Sun Park,Jin-Heui Kang,Hee-Wan Choi,Je-Eul Nou,Ill-Sup Lee,Soon Youl Kang,Kwon-Kyoo
author_sort	Jung,Yu-Jin
title	Overexpression of the pineapple fruit bromelain gene (BAA) in transgenic Chinese cabbage (Brassica rapa) results in enhanced resistance to bacterial soft rot
title_short	Overexpression of the pineapple fruit bromelain gene (BAA) in transgenic Chinese cabbage (Brassica rapa) results in enhanced resistance to bacterial soft rot
title_full	Overexpression of the pineapple fruit bromelain gene (BAA) in transgenic Chinese cabbage (Brassica rapa) results in enhanced resistance to bacterial soft rot
title_fullStr	Overexpression of the pineapple fruit bromelain gene (BAA) in transgenic Chinese cabbage (Brassica rapa) results in enhanced resistance to bacterial soft rot
title_full_unstemmed	Overexpression of the pineapple fruit bromelain gene (BAA) in transgenic Chinese cabbage (Brassica rapa) results in enhanced resistance to bacterial soft rot
title_sort	overexpression of the pineapple fruit bromelain gene (baa) in transgenic chinese cabbage (brassica rapa) results in enhanced resistance to bacterial soft rot
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2008
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000100007
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Overexpression of the pineapple fruit bromelain gene (BAA) in transgenic Chinese cabbage (Brassica rapa) results in enhanced resistance to bacterial soft rot

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