Plant regeneration via indirect somatic embryogenesis and optimisation of genetic transformation in Coffea arabica L. cvs. Caturra and Catuaí

Plant regeneration via indirect somatic embryogenesis and optimisation of genetic transformation in Coffea arabica L. cvs. Caturra and Catuaí

A protocol for Coffea arabica L. cvs. Caturra and Catuaí plant regeneration via indirect somatic embryogenesis (ISE) was established. Furthermore, a biolistic mediated genetic transformation protocol was optimized for Catuaí callus aggregates. Maximum callus induction was obtained when Caturra (87%)...

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Autores principales: Gatica-Arias,Andrés M, Arrieta-Espinoza,Griselda, Espinoza Esquivel,Ana M
Lenguaje:English
Publicado: Pontificia Universidad Católica de Valparaíso 2008
Materias:
bioreactor
coffee
genetic transformation
somatic embryogenesis
suspension cultures
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000100010
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