Role of PPase-SE in Geotrichum klebahnii, a yeast-like fungus able to solubilize pectin

Protopectinases (PPases) constitute a heterogeneous group of extracellular enzymes able to release soluble pectin from insoluble protopectin in plant tissues. Geotrichum klebahnii (ATCC 42397) produces PPase-SE with endopolygalacturonase activity. PPase-SE has been used for pectin extraction and mac...

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Autores principales:	Rojas,Natalia Lorena, Cavalitto,Sebastián Fernando, Mignone,Carlos Fernando, Hours,Roque Alberto
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2008
Materias:	carbon balance enzymatic pectin extraction microbial pectin utilization pectinases synthetic medium
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000100012
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spelling	oai:scielo:S0717-345820080001000122008-10-28Role of PPase-SE in Geotrichum klebahnii, a yeast-like fungus able to solubilize pectinRojas,Natalia LorenaCavalitto,Sebastián FernandoMignone,Carlos FernandoHours,Roque Alberto carbon balance enzymatic pectin extraction microbial pectin utilization pectinases synthetic medium Protopectinases (PPases) constitute a heterogeneous group of extracellular enzymes able to release soluble pectin from insoluble protopectin in plant tissues. Geotrichum klebahnii (ATCC 42397) produces PPase-SE with endopolygalacturonase activity. PPase-SE has been used for pectin extraction and maceration of plant tissues. Here, the capacity of G. klebahnii to use different pectins as carbon and energy sources (CES) was studied, in addition to PPase-SE capacity to release pectin from lemon peel. The strain was unable to use pectin from different origins as CES. When G. klebahnii was cultivated with mixtures of different amounts of glucose and citrus pectin as CES, the biomass obtained was proportional to the initial concentration of glucose, which was completely consumed. In addition, it produced PPase-SE in a glucose-containing medium. A culture was used for the extraction of pectin from lemon peels. Pectin was enzymatically extracted simultaneously with tissue maceration, yielding 3.7 g of (dry) pectin per 100 g of (wet) lemon peel. Extracted pectin was not metabolized by the strain. It was concluded that G. klebahnii uses PPase-SE to macerate, invade and colonize plant tissues, thus releasing soluble sugars to be used as CES without metabolizing solubilized pectin.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.11 n.1 20082008-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000100012en10.4067/S0717-34582008000100012
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	carbon balance enzymatic pectin extraction microbial pectin utilization pectinases synthetic medium
spellingShingle	carbon balance enzymatic pectin extraction microbial pectin utilization pectinases synthetic medium Rojas,Natalia Lorena Cavalitto,Sebastián Fernando Mignone,Carlos Fernando Hours,Roque Alberto Role of PPase-SE in Geotrichum klebahnii, a yeast-like fungus able to solubilize pectin
description	Protopectinases (PPases) constitute a heterogeneous group of extracellular enzymes able to release soluble pectin from insoluble protopectin in plant tissues. Geotrichum klebahnii (ATCC 42397) produces PPase-SE with endopolygalacturonase activity. PPase-SE has been used for pectin extraction and maceration of plant tissues. Here, the capacity of G. klebahnii to use different pectins as carbon and energy sources (CES) was studied, in addition to PPase-SE capacity to release pectin from lemon peel. The strain was unable to use pectin from different origins as CES. When G. klebahnii was cultivated with mixtures of different amounts of glucose and citrus pectin as CES, the biomass obtained was proportional to the initial concentration of glucose, which was completely consumed. In addition, it produced PPase-SE in a glucose-containing medium. A culture was used for the extraction of pectin from lemon peels. Pectin was enzymatically extracted simultaneously with tissue maceration, yielding 3.7 g of (dry) pectin per 100 g of (wet) lemon peel. Extracted pectin was not metabolized by the strain. It was concluded that G. klebahnii uses PPase-SE to macerate, invade and colonize plant tissues, thus releasing soluble sugars to be used as CES without metabolizing solubilized pectin.
author	Rojas,Natalia Lorena Cavalitto,Sebastián Fernando Mignone,Carlos Fernando Hours,Roque Alberto
author_facet	Rojas,Natalia Lorena Cavalitto,Sebastián Fernando Mignone,Carlos Fernando Hours,Roque Alberto
author_sort	Rojas,Natalia Lorena
title	Role of PPase-SE in Geotrichum klebahnii, a yeast-like fungus able to solubilize pectin
title_short	Role of PPase-SE in Geotrichum klebahnii, a yeast-like fungus able to solubilize pectin
title_full	Role of PPase-SE in Geotrichum klebahnii, a yeast-like fungus able to solubilize pectin
title_fullStr	Role of PPase-SE in Geotrichum klebahnii, a yeast-like fungus able to solubilize pectin
title_full_unstemmed	Role of PPase-SE in Geotrichum klebahnii, a yeast-like fungus able to solubilize pectin
title_sort	role of ppase-se in geotrichum klebahnii, a yeast-like fungus able to solubilize pectin
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2008
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000100012
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Role of PPase-SE in Geotrichum klebahnii, a yeast-like fungus able to solubilize pectin

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