Agrobacterium-mediated transient transformation of Mexican prickly poppy (Argemone mexicana L.)

Shoot apex, leaf primordia, leaf sections and roots from Mexican prickly poppy seedlings, were inoculated with Agrobacterium tumefaciens harboring the binary vector pCAMBIA2301, which contained the β-glucuronidase (uid A) gene. Histochemical β-glucuronidase (GUS) assay in infected...

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Autores principales:	Godoy-Hernández,Gregorio, Avilés-Berzunza,Elidé, Carrillo-Pech,Mildred, Vázquez-Flota,Felipe
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2008
Materias:	Argemone mexicana β-glucuronidase benzylisoquinoline alkaloids genetic transformation neomycin phosphotransferase II
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000100013
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spelling	oai:scielo:S0717-345820080001000132008-10-28Agrobacterium-mediated transient transformation of Mexican prickly poppy (Argemone mexicana L.)Godoy-Hernández,GregorioAvilés-Berzunza,ElidéCarrillo-Pech,MildredVázquez-Flota,Felipe Argemone mexicana β-glucuronidase benzylisoquinoline alkaloids genetic transformation neomycin phosphotransferase II Shoot apex, leaf primordia, leaf sections and roots from Mexican prickly poppy seedlings, were inoculated with Agrobacterium tumefaciens harboring the binary vector pCAMBIA2301, which contained the β-glucuronidase (uid A) gene. Histochemical β-glucuronidase (GUS) assay in infected explants showed transient gus gene expression between 3 and 12 days after inoculation. To our knowledge, this is the first report of A. mexicana susceptibility to A. tumefaciens-mediated genetic transformationinfo:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.11 n.1 20082008-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000100013en10.4067/S0717-34582008000100013
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Argemone mexicana β-glucuronidase benzylisoquinoline alkaloids genetic transformation neomycin phosphotransferase II
spellingShingle	Argemone mexicana β-glucuronidase benzylisoquinoline alkaloids genetic transformation neomycin phosphotransferase II Godoy-Hernández,Gregorio Avilés-Berzunza,Elidé Carrillo-Pech,Mildred Vázquez-Flota,Felipe Agrobacterium-mediated transient transformation of Mexican prickly poppy (Argemone mexicana L.)
description	Shoot apex, leaf primordia, leaf sections and roots from Mexican prickly poppy seedlings, were inoculated with Agrobacterium tumefaciens harboring the binary vector pCAMBIA2301, which contained the β-glucuronidase (uid A) gene. Histochemical β-glucuronidase (GUS) assay in infected explants showed transient gus gene expression between 3 and 12 days after inoculation. To our knowledge, this is the first report of A. mexicana susceptibility to A. tumefaciens-mediated genetic transformation
author	Godoy-Hernández,Gregorio Avilés-Berzunza,Elidé Carrillo-Pech,Mildred Vázquez-Flota,Felipe
author_facet	Godoy-Hernández,Gregorio Avilés-Berzunza,Elidé Carrillo-Pech,Mildred Vázquez-Flota,Felipe
author_sort	Godoy-Hernández,Gregorio
title	Agrobacterium-mediated transient transformation of Mexican prickly poppy (Argemone mexicana L.)
title_short	Agrobacterium-mediated transient transformation of Mexican prickly poppy (Argemone mexicana L.)
title_full	Agrobacterium-mediated transient transformation of Mexican prickly poppy (Argemone mexicana L.)
title_fullStr	Agrobacterium-mediated transient transformation of Mexican prickly poppy (Argemone mexicana L.)
title_full_unstemmed	Agrobacterium-mediated transient transformation of Mexican prickly poppy (Argemone mexicana L.)
title_sort	agrobacterium-mediated transient transformation of mexican prickly poppy (argemone mexicana l.)
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2008
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000100013
work_keys_str_mv	AT godoyhernandezgregorio agrobacteriummediatedtransienttransformationofmexicanpricklypoppyargemonemexicanal AT avilesberzunzaelide agrobacteriummediatedtransienttransformationofmexicanpricklypoppyargemonemexicanal AT carrillopechmildred agrobacteriummediatedtransienttransformationofmexicanpricklypoppyargemonemexicanal AT vazquezflotafelipe agrobacteriummediatedtransienttransformationofmexicanpricklypoppyargemonemexicanal
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Agrobacterium-mediated transient transformation of Mexican prickly poppy (Argemone mexicana L.)

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