Generation and analysis of an Eucalyptus globulus cDNA library constructed from seedlings subjected to low temperature conditions

Eucalyptus globulus is the most important commercial temperate hardwood in the world because of its wood properties and due to its characteristics for biofuel production. However, only a very low number of expressed sequence tags (ESTs) are publicly available for this tree species. We constructed a...

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Autores principales:	Rasmussen-Poblete,Susana, Valdes,Jorge, Gamboa,Maria Cecilia, Valenzuela,Pablo D.T, Krauskopf,Erwin
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2008
Materias:	cellulose cold-temperature EST database forest biotechnology lignin
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000200008
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spelling	oai:scielo:S0717-345820080002000082009-01-21Generation and analysis of an Eucalyptus globulus cDNA library constructed from seedlings subjected to low temperature conditionsRasmussen-Poblete,SusanaValdes,JorgeGamboa,Maria CeciliaValenzuela,Pablo D.TKrauskopf,Erwin cellulose cold-temperature EST database forest biotechnology lignin Eucalyptus globulus is the most important commercial temperate hardwood in the world because of its wood properties and due to its characteristics for biofuel production. However, only a very low number of expressed sequence tags (ESTs) are publicly available for this tree species. We constructed a cDNA from E. globulus seedlings subjected to low temperature and sequenced 9,913 randomly selected clones, generating 8,737 curated ESTs. The assembly produced 1,062 contigs and 3,879 singletons forming a Eucalyptus unigene set. Based on BLASTX analysis, 89.3% of the contigs and 88.5% of the singletons had significant similarity to known genes in the non-redundant database of GenBank. The Eucalyptus unigene set generated is a valuable public resource that provides an initial model for genes and regulatory pathways involved in cell wall biosynthesis at low temperature.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.11 n.2 20082008-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000200008en10.4067/S0717-34582008000200008
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	cellulose cold-temperature EST database forest biotechnology lignin
spellingShingle	cellulose cold-temperature EST database forest biotechnology lignin Rasmussen-Poblete,Susana Valdes,Jorge Gamboa,Maria Cecilia Valenzuela,Pablo D.T Krauskopf,Erwin Generation and analysis of an Eucalyptus globulus cDNA library constructed from seedlings subjected to low temperature conditions
description	Eucalyptus globulus is the most important commercial temperate hardwood in the world because of its wood properties and due to its characteristics for biofuel production. However, only a very low number of expressed sequence tags (ESTs) are publicly available for this tree species. We constructed a cDNA from E. globulus seedlings subjected to low temperature and sequenced 9,913 randomly selected clones, generating 8,737 curated ESTs. The assembly produced 1,062 contigs and 3,879 singletons forming a Eucalyptus unigene set. Based on BLASTX analysis, 89.3% of the contigs and 88.5% of the singletons had significant similarity to known genes in the non-redundant database of GenBank. The Eucalyptus unigene set generated is a valuable public resource that provides an initial model for genes and regulatory pathways involved in cell wall biosynthesis at low temperature.
author	Rasmussen-Poblete,Susana Valdes,Jorge Gamboa,Maria Cecilia Valenzuela,Pablo D.T Krauskopf,Erwin
author_facet	Rasmussen-Poblete,Susana Valdes,Jorge Gamboa,Maria Cecilia Valenzuela,Pablo D.T Krauskopf,Erwin
author_sort	Rasmussen-Poblete,Susana
title	Generation and analysis of an Eucalyptus globulus cDNA library constructed from seedlings subjected to low temperature conditions
title_short	Generation and analysis of an Eucalyptus globulus cDNA library constructed from seedlings subjected to low temperature conditions
title_full	Generation and analysis of an Eucalyptus globulus cDNA library constructed from seedlings subjected to low temperature conditions
title_fullStr	Generation and analysis of an Eucalyptus globulus cDNA library constructed from seedlings subjected to low temperature conditions
title_full_unstemmed	Generation and analysis of an Eucalyptus globulus cDNA library constructed from seedlings subjected to low temperature conditions
title_sort	generation and analysis of an eucalyptus globulus cdna library constructed from seedlings subjected to low temperature conditions
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2008
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000200008
work_keys_str_mv	AT rasmussenpobletesusana generationandanalysisofaneucalyptusglobuluscdnalibraryconstructedfromseedlingssubjectedtolowtemperatureconditions AT valdesjorge generationandanalysisofaneucalyptusglobuluscdnalibraryconstructedfromseedlingssubjectedtolowtemperatureconditions AT gamboamariacecilia generationandanalysisofaneucalyptusglobuluscdnalibraryconstructedfromseedlingssubjectedtolowtemperatureconditions AT valenzuelapablodt generationandanalysisofaneucalyptusglobuluscdnalibraryconstructedfromseedlingssubjectedtolowtemperatureconditions AT krauskopferwin generationandanalysisofaneucalyptusglobuluscdnalibraryconstructedfromseedlingssubjectedtolowtemperatureconditions
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Generation and analysis of an Eucalyptus globulus cDNA library constructed from seedlings subjected to low temperature conditions

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