Identification of unknown genetically modified material admixed in conventional cotton seed and development of an event-specific detection method
Entering the second decade of commercialization of biotech crops, the global area cultivated with transgenic plants constantly expands and national legislations in many countries, particularly in the European Union, require identification and labeling of genetically modified material in food and fee...
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| Lenguaje: | English |
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Pontificia Universidad Católica de Valparaíso
2008
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oai:scielo:S0717-345820080002000102009-01-21Identification of unknown genetically modified material admixed in conventional cotton seed and development of an event-specific detection methodAkritidis,PaschalisPasentsis,KonstantinosTsaftaris,Athanasios SMylona,Photini VPolidoros,Alexios N event specific detection genetically modified cotton genome walking GMO detection Mon1445 Entering the second decade of commercialization of biotech crops, the global area cultivated with transgenic plants constantly expands and national legislations in many countries, particularly in the European Union, require identification and labeling of genetically modified material in food and feed. We describe here a procedure for characterizing transgenic material of unknown origin present in conventional seed lots using a genome walking strategy for isolation and characterization of the junction between the inserted transgene construct and the host plant genomic DNA. The procedure was applied to transgenic cotton detected as adventitious or technically unavoidable presence in a conventional commercial cultivar. The structure of the isolated region revealed that the transgenic material derived from Monsanto’s event 1445 transgenic cotton. Due to the random incorporation of the transgene into the host plant’s genome, the sequence of the junction region obtained using the genome walking strategy, provided the means to develop an event-specific identification method without prior knowledge for the nature of the transformation event. Thus, we documented a methodology for developing an event-specific detection protocol even without prior knowledge of the genetic modification event.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.11 n.2 20082008-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000200010en10.4067/S0717-34582008000200010 |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| topic |
event specific detection genetically modified cotton genome walking GMO detection Mon1445 |
| spellingShingle |
event specific detection genetically modified cotton genome walking GMO detection Mon1445 Akritidis,Paschalis Pasentsis,Konstantinos Tsaftaris,Athanasios S Mylona,Photini V Polidoros,Alexios N Identification of unknown genetically modified material admixed in conventional cotton seed and development of an event-specific detection method |
| description |
Entering the second decade of commercialization of biotech crops, the global area cultivated with transgenic plants constantly expands and national legislations in many countries, particularly in the European Union, require identification and labeling of genetically modified material in food and feed. We describe here a procedure for characterizing transgenic material of unknown origin present in conventional seed lots using a genome walking strategy for isolation and characterization of the junction between the inserted transgene construct and the host plant genomic DNA. The procedure was applied to transgenic cotton detected as adventitious or technically unavoidable presence in a conventional commercial cultivar. The structure of the isolated region revealed that the transgenic material derived from Monsanto’s event 1445 transgenic cotton. Due to the random incorporation of the transgene into the host plant’s genome, the sequence of the junction region obtained using the genome walking strategy, provided the means to develop an event-specific identification method without prior knowledge for the nature of the transformation event. Thus, we documented a methodology for developing an event-specific detection protocol even without prior knowledge of the genetic modification event. |
| author |
Akritidis,Paschalis Pasentsis,Konstantinos Tsaftaris,Athanasios S Mylona,Photini V Polidoros,Alexios N |
| author_facet |
Akritidis,Paschalis Pasentsis,Konstantinos Tsaftaris,Athanasios S Mylona,Photini V Polidoros,Alexios N |
| author_sort |
Akritidis,Paschalis |
| title |
Identification of unknown genetically modified material admixed in conventional cotton seed and development of an event-specific detection method |
| title_short |
Identification of unknown genetically modified material admixed in conventional cotton seed and development of an event-specific detection method |
| title_full |
Identification of unknown genetically modified material admixed in conventional cotton seed and development of an event-specific detection method |
| title_fullStr |
Identification of unknown genetically modified material admixed in conventional cotton seed and development of an event-specific detection method |
| title_full_unstemmed |
Identification of unknown genetically modified material admixed in conventional cotton seed and development of an event-specific detection method |
| title_sort |
identification of unknown genetically modified material admixed in conventional cotton seed and development of an event-specific detection method |
| publisher |
Pontificia Universidad Católica de Valparaíso |
| publishDate |
2008 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000200010 |
| work_keys_str_mv |
AT akritidispaschalis identificationofunknowngeneticallymodifiedmaterialadmixedinconventionalcottonseedanddevelopmentofaneventspecificdetectionmethod AT pasentsiskonstantinos identificationofunknowngeneticallymodifiedmaterialadmixedinconventionalcottonseedanddevelopmentofaneventspecificdetectionmethod AT tsaftarisathanasioss identificationofunknowngeneticallymodifiedmaterialadmixedinconventionalcottonseedanddevelopmentofaneventspecificdetectionmethod AT mylonaphotiniv identificationofunknowngeneticallymodifiedmaterialadmixedinconventionalcottonseedanddevelopmentofaneventspecificdetectionmethod AT polidorosalexiosn identificationofunknowngeneticallymodifiedmaterialadmixedinconventionalcottonseedanddevelopmentofaneventspecificdetectionmethod |
| _version_ |
1718441783990419456 |