Induction of in vitro flowering in Capsicum frutescens under the influence of silver nitrate and cobalt chloride and pollen transformation

The influence of silver nitrate (AgNO3) and cobalt chloride (CoCl2) on shoot multiplication and in vitro flowering in Capsicum frutescens Mill. was investigated. Exogenous administration of AgNO3 and CoCl2 at a concentration of 30 µM resulted in the maximum tissue response in terms of shoot length a...

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Autores principales:	Sharma,Ashwani, Kumar,Vinod, Giridhar,Parvatam, Ravishankar,Gokare Aswathanarayana
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2008
Materias:	Capsicum frutescens cobalt chloride in vitro flowering pollen transformation regeneration silver nitrate
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000200011
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spelling	oai:scielo:S0717-345820080002000112009-01-21Induction of in vitro flowering in Capsicum frutescens under the influence of silver nitrate and cobalt chloride and pollen transformationSharma,AshwaniKumar,VinodGiridhar,ParvatamRavishankar,Gokare Aswathanarayana Capsicum frutescens cobalt chloride in vitro flowering pollen transformation regeneration silver nitrate The influence of silver nitrate (AgNO3) and cobalt chloride (CoCl2) on shoot multiplication and in vitro flowering in Capsicum frutescens Mill. was investigated. Exogenous administration of AgNO3 and CoCl2 at a concentration of 30 µM resulted in the maximum tissue response in terms of shoot length and number of shoots after 45 days culturing on MS medium. Both silver nitrate (40 µM) and cobalt chloride (30 µM) influenced in vitro flowering after 25 and 45 days respectively. This is the first report on in vitro flowering in C. frutescens. The study also demonstrated successful transformation of pollen obtained from the in vitro flowers. Since capsicum is highly recalcitrant to in vitro plant regeneration, the results of the study may be highly useful in transformation of capsicum using germ free in vitro flowers.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.11 n.2 20082008-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000200011en10.4067/S0717-34582008000200011
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Capsicum frutescens cobalt chloride in vitro flowering pollen transformation regeneration silver nitrate
spellingShingle	Capsicum frutescens cobalt chloride in vitro flowering pollen transformation regeneration silver nitrate Sharma,Ashwani Kumar,Vinod Giridhar,Parvatam Ravishankar,Gokare Aswathanarayana Induction of in vitro flowering in Capsicum frutescens under the influence of silver nitrate and cobalt chloride and pollen transformation
description	The influence of silver nitrate (AgNO3) and cobalt chloride (CoCl2) on shoot multiplication and in vitro flowering in Capsicum frutescens Mill. was investigated. Exogenous administration of AgNO3 and CoCl2 at a concentration of 30 µM resulted in the maximum tissue response in terms of shoot length and number of shoots after 45 days culturing on MS medium. Both silver nitrate (40 µM) and cobalt chloride (30 µM) influenced in vitro flowering after 25 and 45 days respectively. This is the first report on in vitro flowering in C. frutescens. The study also demonstrated successful transformation of pollen obtained from the in vitro flowers. Since capsicum is highly recalcitrant to in vitro plant regeneration, the results of the study may be highly useful in transformation of capsicum using germ free in vitro flowers.
author	Sharma,Ashwani Kumar,Vinod Giridhar,Parvatam Ravishankar,Gokare Aswathanarayana
author_facet	Sharma,Ashwani Kumar,Vinod Giridhar,Parvatam Ravishankar,Gokare Aswathanarayana
author_sort	Sharma,Ashwani
title	Induction of in vitro flowering in Capsicum frutescens under the influence of silver nitrate and cobalt chloride and pollen transformation
title_short	Induction of in vitro flowering in Capsicum frutescens under the influence of silver nitrate and cobalt chloride and pollen transformation
title_full	Induction of in vitro flowering in Capsicum frutescens under the influence of silver nitrate and cobalt chloride and pollen transformation
title_fullStr	Induction of in vitro flowering in Capsicum frutescens under the influence of silver nitrate and cobalt chloride and pollen transformation
title_full_unstemmed	Induction of in vitro flowering in Capsicum frutescens under the influence of silver nitrate and cobalt chloride and pollen transformation
title_sort	induction of in vitro flowering in capsicum frutescens under the influence of silver nitrate and cobalt chloride and pollen transformation
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2008
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000200011
work_keys_str_mv	AT sharmaashwani inductionofinvitrofloweringincapsicumfrutescensundertheinfluenceofsilvernitrateandcobaltchlorideandpollentransformation AT kumarvinod inductionofinvitrofloweringincapsicumfrutescensundertheinfluenceofsilvernitrateandcobaltchlorideandpollentransformation AT giridharparvatam inductionofinvitrofloweringincapsicumfrutescensundertheinfluenceofsilvernitrateandcobaltchlorideandpollentransformation AT ravishankargokareaswathanarayana inductionofinvitrofloweringincapsicumfrutescensundertheinfluenceofsilvernitrateandcobaltchlorideandpollentransformation
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Induction of in vitro flowering in Capsicum frutescens under the influence of silver nitrate and cobalt chloride and pollen transformation

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