Physiological-enzymatic characteristics and inoculation of mycelial strains of Descolea antarctica Sing. in Nothofagus seedlings

At present, reforestation has focused on native forests with anthropogenic intervention and eroded soils. There is interest in producing Nothofagus seedlings which can overcome adverse conditions encountered on reforestation sites. It is necessary to find new fungi that can be utilized as mycorrhiza...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Valenzuela,Eduardo, Hipp,Joseline, Alonso,Claudia, Godoy,Roberto, Alberdi,Miren, Alvarez,Maricel, Saavedra,Isabel
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2008
Materias:	cellulose ectomycorrhizal fungi Nothofagus pH phosphatase temperature
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000200012
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0717-34582008000200012
record_format	dspace
spelling	oai:scielo:S0717-345820080002000122009-01-21Physiological-enzymatic characteristics and inoculation of mycelial strains of Descolea antarctica Sing. in Nothofagus seedlingsValenzuela,EduardoHipp,JoselineAlonso,ClaudiaGodoy,RobertoAlberdi,MirenAlvarez,MaricelSaavedra,Isabel cellulose ectomycorrhizal fungi Nothofagus pH phosphatase temperature At present, reforestation has focused on native forests with anthropogenic intervention and eroded soils. There is interest in producing Nothofagus seedlings which can overcome adverse conditions encountered on reforestation sites. It is necessary to find new fungi that can be utilized as mycorrhizal inoculants and that enable the seedlings to increase their tolerance to adverse conditions. Two ectomycorrhizal strains of the fungus Descolea antarctica (D1 and D2) were cultured at different temperatures, pH levels and the activities of amylases, cellulases, and phosphatases were determined. In greenhouse and nursery trials, the growth responses of inoculated Nothofagus obliqua seedlings were evaluated. D1 and D2 exhibited the highest growth rates at 23ºC. Both strains grew at pH levels from 4 to 11. The highest enzymatic activities were registered for amylase (57.2 mg glucose/ml * g of mycelium * hr) and acid phosphatases (58.1 mg p-nitrophenol/ml * g of mycelium * hr) at 37ºC, and acid phospatases (1.720 mg p-nitrophenol/ml * g of mycelium * hr) and alkaline phosphatases (1.360 mg p-nitrophenol/ml * g of mycelium * hr) at pH 4 and pH 11, respectively. We conclude that suitable N. obliqua seedlings for use in reforestation were obtained using D2 as inoculant.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.11 n.2 20082008-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000200012en10.4067/S0717-34582008000200012
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	cellulose ectomycorrhizal fungi Nothofagus pH phosphatase temperature
spellingShingle	cellulose ectomycorrhizal fungi Nothofagus pH phosphatase temperature Valenzuela,Eduardo Hipp,Joseline Alonso,Claudia Godoy,Roberto Alberdi,Miren Alvarez,Maricel Saavedra,Isabel Physiological-enzymatic characteristics and inoculation of mycelial strains of Descolea antarctica Sing. in Nothofagus seedlings
description	At present, reforestation has focused on native forests with anthropogenic intervention and eroded soils. There is interest in producing Nothofagus seedlings which can overcome adverse conditions encountered on reforestation sites. It is necessary to find new fungi that can be utilized as mycorrhizal inoculants and that enable the seedlings to increase their tolerance to adverse conditions. Two ectomycorrhizal strains of the fungus Descolea antarctica (D1 and D2) were cultured at different temperatures, pH levels and the activities of amylases, cellulases, and phosphatases were determined. In greenhouse and nursery trials, the growth responses of inoculated Nothofagus obliqua seedlings were evaluated. D1 and D2 exhibited the highest growth rates at 23ºC. Both strains grew at pH levels from 4 to 11. The highest enzymatic activities were registered for amylase (57.2 mg glucose/ml * g of mycelium * hr) and acid phosphatases (58.1 mg p-nitrophenol/ml * g of mycelium * hr) at 37ºC, and acid phospatases (1.720 mg p-nitrophenol/ml * g of mycelium * hr) and alkaline phosphatases (1.360 mg p-nitrophenol/ml * g of mycelium * hr) at pH 4 and pH 11, respectively. We conclude that suitable N. obliqua seedlings for use in reforestation were obtained using D2 as inoculant.
author	Valenzuela,Eduardo Hipp,Joseline Alonso,Claudia Godoy,Roberto Alberdi,Miren Alvarez,Maricel Saavedra,Isabel
author_facet	Valenzuela,Eduardo Hipp,Joseline Alonso,Claudia Godoy,Roberto Alberdi,Miren Alvarez,Maricel Saavedra,Isabel
author_sort	Valenzuela,Eduardo
title	Physiological-enzymatic characteristics and inoculation of mycelial strains of Descolea antarctica Sing. in Nothofagus seedlings
title_short	Physiological-enzymatic characteristics and inoculation of mycelial strains of Descolea antarctica Sing. in Nothofagus seedlings
title_full	Physiological-enzymatic characteristics and inoculation of mycelial strains of Descolea antarctica Sing. in Nothofagus seedlings
title_fullStr	Physiological-enzymatic characteristics and inoculation of mycelial strains of Descolea antarctica Sing. in Nothofagus seedlings
title_full_unstemmed	Physiological-enzymatic characteristics and inoculation of mycelial strains of Descolea antarctica Sing. in Nothofagus seedlings
title_sort	physiological-enzymatic characteristics and inoculation of mycelial strains of descolea antarctica sing. in nothofagus seedlings
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2008
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000200012
work_keys_str_mv	AT valenzuelaeduardo physiologicalenzymaticcharacteristicsandinoculationofmycelialstrainsofdescoleaantarcticasinginnothofagusseedlings AT hippjoseline physiologicalenzymaticcharacteristicsandinoculationofmycelialstrainsofdescoleaantarcticasinginnothofagusseedlings AT alonsoclaudia physiologicalenzymaticcharacteristicsandinoculationofmycelialstrainsofdescoleaantarcticasinginnothofagusseedlings AT godoyroberto physiologicalenzymaticcharacteristicsandinoculationofmycelialstrainsofdescoleaantarcticasinginnothofagusseedlings AT alberdimiren physiologicalenzymaticcharacteristicsandinoculationofmycelialstrainsofdescoleaantarcticasinginnothofagusseedlings AT alvarezmaricel physiologicalenzymaticcharacteristicsandinoculationofmycelialstrainsofdescoleaantarcticasinginnothofagusseedlings AT saavedraisabel physiologicalenzymaticcharacteristicsandinoculationofmycelialstrainsofdescoleaantarcticasinginnothofagusseedlings
_version_	1718441784436064256

Physiological-enzymatic characteristics and inoculation of mycelial strains of Descolea antarctica Sing. in Nothofagus seedlings

Ejemplares similares