Universal protocol for generating 100bp size standard for endless usage

Developing countries are facing severe bottlenecks in the technological advancement in biotechnology, due to restrictions imposed by patent protected products and protocols. This calls for designing of simple and cost-effective alternatives for the indispensable products like DNA molecular weight ma...

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Autores principales:	Natarajan,Chandrasekhar, Vijayan,Neetha N, Vaidyan,Linda Koshy, Mathew,Anila, Srinivas,Lekshmy, Banerjee,Moinak
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2008
Materias:	100bp ladder cost effective DNA size standards endless usage
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000200015
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spelling	oai:scielo:S0717-345820080002000152009-01-21Universal protocol for generating 100bp size standard for endless usageNatarajan,ChandrasekharVijayan,Neetha NVaidyan,Linda KoshyMathew,AnilaSrinivas,LekshmyBanerjee,Moinak 100bp ladder cost effective DNA size standards endless usage Developing countries are facing severe bottlenecks in the technological advancement in biotechnology, due to restrictions imposed by patent protected products and protocols. This calls for designing of simple and cost-effective alternatives for the indispensable products like DNA molecular weight markers. We demonstrate a novel, rapid and cost-effective method of making in-house 100bp ladder for routine use. In our method we use a single forward primer and five reverse primers designed on the backbone sequence of a commonly used vector template. These primers are used at a universal annealing temperature to amplify ten DNA fragments of accurate size ranging from 100bp to 1000bp. Our PCR-based method can provide size standards for an endless usage.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.11 n.2 20082008-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000200015en10.4067/S0717-34582008000200015
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	100bp ladder cost effective DNA size standards endless usage
spellingShingle	100bp ladder cost effective DNA size standards endless usage Natarajan,Chandrasekhar Vijayan,Neetha N Vaidyan,Linda Koshy Mathew,Anila Srinivas,Lekshmy Banerjee,Moinak Universal protocol for generating 100bp size standard for endless usage
description	Developing countries are facing severe bottlenecks in the technological advancement in biotechnology, due to restrictions imposed by patent protected products and protocols. This calls for designing of simple and cost-effective alternatives for the indispensable products like DNA molecular weight markers. We demonstrate a novel, rapid and cost-effective method of making in-house 100bp ladder for routine use. In our method we use a single forward primer and five reverse primers designed on the backbone sequence of a commonly used vector template. These primers are used at a universal annealing temperature to amplify ten DNA fragments of accurate size ranging from 100bp to 1000bp. Our PCR-based method can provide size standards for an endless usage.
author	Natarajan,Chandrasekhar Vijayan,Neetha N Vaidyan,Linda Koshy Mathew,Anila Srinivas,Lekshmy Banerjee,Moinak
author_facet	Natarajan,Chandrasekhar Vijayan,Neetha N Vaidyan,Linda Koshy Mathew,Anila Srinivas,Lekshmy Banerjee,Moinak
author_sort	Natarajan,Chandrasekhar
title	Universal protocol for generating 100bp size standard for endless usage
title_short	Universal protocol for generating 100bp size standard for endless usage
title_full	Universal protocol for generating 100bp size standard for endless usage
title_fullStr	Universal protocol for generating 100bp size standard for endless usage
title_full_unstemmed	Universal protocol for generating 100bp size standard for endless usage
title_sort	universal protocol for generating 100bp size standard for endless usage
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2008
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000200015
work_keys_str_mv	AT natarajanchandrasekhar universalprotocolforgenerating100bpsizestandardforendlessusage AT vijayanneethan universalprotocolforgenerating100bpsizestandardforendlessusage AT vaidyanlindakoshy universalprotocolforgenerating100bpsizestandardforendlessusage AT mathewanila universalprotocolforgenerating100bpsizestandardforendlessusage AT srinivaslekshmy universalprotocolforgenerating100bpsizestandardforendlessusage AT banerjeemoinak universalprotocolforgenerating100bpsizestandardforendlessusage
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Universal protocol for generating 100bp size standard for endless usage

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