Biotransformation of 3-hydroxydibenzo-α-pyrone into 3,8 dihydroxydibenzo-α-pyrone and aminoacyl conjugates by Aspergillus niger isolated from native“shilajit”
“Shilajit” is a panacea in Ayurveda, the Indian traditional system of medicine. The major bioactives of “shilajit” have been identified as dibenzo-α-pyrones (DBPs), its oligomers and aminoacyl conjugated derivatives. These bioactive compounds play a c...
Guardado en:
| Autores principales: | , , , , , |
|---|---|
| Lenguaje: | English |
| Publicado: |
Pontificia Universidad Católica de Valparaíso
2008
|
| Materias: | |
| Acceso en línea: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000300001 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:scielo:S0717-34582008000300001 |
|---|---|
| record_format |
dspace |
| spelling |
oai:scielo:S0717-345820080003000012009-01-30Biotransformation of 3-hydroxydibenzo-α-pyrone into 3,8 dihydroxydibenzo-α-pyrone and aminoacyl conjugates by Aspergillus niger isolated from native“shilajit”Islam,AminulGhosh,RunaBanerjee,DipankarNath,PialiMazumder,Upal KantiGhosal,Shibnath Coenzyme Q10 reduction electron transport chain humification paleohumus “Shilajit” is a panacea in Ayurveda, the Indian traditional system of medicine. The major bioactives of “shilajit” have been identified as dibenzo-α-pyrones (DBPs), its oligomers and aminoacyl conjugated derivatives. These bioactive compounds play a crucial role in energy metabolism in all animal cells including those of man. 3-hydroxydibenzo-α-pyrone (3-OH-DBP), a key DBP component of “shilajit” is converted, among other products, to another active DBP derivative, viz. 3,8-hydroxydibenzo-α-pyrone, 3,8(OH)2-DBP, in vivo, when its precursor is ingested. 3,8(OH)2-DBP is then involved in energy synthesis in the mitochondria in the reduction and stabilization of coenzyme Q10 in the electron transport chain. As the chemical synthesis of 3,8(OH)2-DBP is a complex, multi-step process and economically not readily viable, we envisioned the development of a process using microorganisms for bioconversion of 3-OH-DBP to 3,8(OH)2-DBP. In this study, the biotransformation of 3-OH-DBP is achieved using Aspergillus niger, which was involved in the humification process on sedimentary rocks leading to “shilajit” formation. A 60% bioconversion of 3-OH-DBP to 3,8(OH)2-DBP and to its aminoacyl derivatives was achieved. The products were characterized and estimated by high performance liquid chromatography (HPLC), high performance flash chromatography (HPFC) and gas chromatography-mass spectrometry (GC-MS) analyses. Among the Aspergillus species isolated and identified from native “shilajit”, A. niger was found to be the most efficient for this bioconversion.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.11 n.3 20082008-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000300001en10.4067/S0717-34582008000300001 |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| topic |
Coenzyme Q10 reduction electron transport chain humification paleohumus |
| spellingShingle |
Coenzyme Q10 reduction electron transport chain humification paleohumus Islam,Aminul Ghosh,Runa Banerjee,Dipankar Nath,Piali Mazumder,Upal Kanti Ghosal,Shibnath Biotransformation of 3-hydroxydibenzo-α-pyrone into 3,8 dihydroxydibenzo-α-pyrone and aminoacyl conjugates by Aspergillus niger isolated from native“shilajit” |
| description |
“Shilajit” is a panacea in Ayurveda, the Indian traditional system of medicine. The major bioactives of “shilajit” have been identified as dibenzo-α-pyrones (DBPs), its oligomers and aminoacyl conjugated derivatives. These bioactive compounds play a crucial role in energy metabolism in all animal cells including those of man. 3-hydroxydibenzo-α-pyrone (3-OH-DBP), a key DBP component of “shilajit” is converted, among other products, to another active DBP derivative, viz. 3,8-hydroxydibenzo-α-pyrone, 3,8(OH)2-DBP, in vivo, when its precursor is ingested. 3,8(OH)2-DBP is then involved in energy synthesis in the mitochondria in the reduction and stabilization of coenzyme Q10 in the electron transport chain. As the chemical synthesis of 3,8(OH)2-DBP is a complex, multi-step process and economically not readily viable, we envisioned the development of a process using microorganisms for bioconversion of 3-OH-DBP to 3,8(OH)2-DBP. In this study, the biotransformation of 3-OH-DBP is achieved using Aspergillus niger, which was involved in the humification process on sedimentary rocks leading to “shilajit” formation. A 60% bioconversion of 3-OH-DBP to 3,8(OH)2-DBP and to its aminoacyl derivatives was achieved. The products were characterized and estimated by high performance liquid chromatography (HPLC), high performance flash chromatography (HPFC) and gas chromatography-mass spectrometry (GC-MS) analyses. Among the Aspergillus species isolated and identified from native “shilajit”, A. niger was found to be the most efficient for this bioconversion. |
| author |
Islam,Aminul Ghosh,Runa Banerjee,Dipankar Nath,Piali Mazumder,Upal Kanti Ghosal,Shibnath |
| author_facet |
Islam,Aminul Ghosh,Runa Banerjee,Dipankar Nath,Piali Mazumder,Upal Kanti Ghosal,Shibnath |
| author_sort |
Islam,Aminul |
| title |
Biotransformation of 3-hydroxydibenzo-α-pyrone into 3,8 dihydroxydibenzo-α-pyrone and aminoacyl conjugates by Aspergillus niger isolated from native“shilajit” |
| title_short |
Biotransformation of 3-hydroxydibenzo-α-pyrone into 3,8 dihydroxydibenzo-α-pyrone and aminoacyl conjugates by Aspergillus niger isolated from native“shilajit” |
| title_full |
Biotransformation of 3-hydroxydibenzo-α-pyrone into 3,8 dihydroxydibenzo-α-pyrone and aminoacyl conjugates by Aspergillus niger isolated from native“shilajit” |
| title_fullStr |
Biotransformation of 3-hydroxydibenzo-α-pyrone into 3,8 dihydroxydibenzo-α-pyrone and aminoacyl conjugates by Aspergillus niger isolated from native“shilajit” |
| title_full_unstemmed |
Biotransformation of 3-hydroxydibenzo-α-pyrone into 3,8 dihydroxydibenzo-α-pyrone and aminoacyl conjugates by Aspergillus niger isolated from native“shilajit” |
| title_sort |
biotransformation of 3-hydroxydibenzo-α-pyrone into 3,8 dihydroxydibenzo-α-pyrone and aminoacyl conjugates by aspergillus niger isolated from native“shilajit” |
| publisher |
Pontificia Universidad Católica de Valparaíso |
| publishDate |
2008 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000300001 |
| work_keys_str_mv |
AT islamaminul biotransformationof3hydroxydibenzo945pyroneinto38dihydroxydibenzo945pyroneandaminoacylconjugatesbyaspergillusnigerisolatedfromnative8220shilajit8221 AT ghoshruna biotransformationof3hydroxydibenzo945pyroneinto38dihydroxydibenzo945pyroneandaminoacylconjugatesbyaspergillusnigerisolatedfromnative8220shilajit8221 AT banerjeedipankar biotransformationof3hydroxydibenzo945pyroneinto38dihydroxydibenzo945pyroneandaminoacylconjugatesbyaspergillusnigerisolatedfromnative8220shilajit8221 AT nathpiali biotransformationof3hydroxydibenzo945pyroneinto38dihydroxydibenzo945pyroneandaminoacylconjugatesbyaspergillusnigerisolatedfromnative8220shilajit8221 AT mazumderupalkanti biotransformationof3hydroxydibenzo945pyroneinto38dihydroxydibenzo945pyroneandaminoacylconjugatesbyaspergillusnigerisolatedfromnative8220shilajit8221 AT ghosalshibnath biotransformationof3hydroxydibenzo945pyroneinto38dihydroxydibenzo945pyroneandaminoacylconjugatesbyaspergillusnigerisolatedfromnative8220shilajit8221 |
| _version_ |
1718441785283313664 |