Characterization of the nuclear ribosomal DNA unit in Oxalis tuberosa (Oxalidacea) and related species

Oxalis tuberosa is an octoploid Andean tuber crop called "oca" that belongs to the worldwide distributed genus Oxalis. The genus is very heterogeneous and its systematics is still problematic. It has been proposed that O. tuberosa evolved by polyploidization of a still not defined ancestor...

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Autores principales:	Tosto,Daniela, Hopp,Esteban
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2008
Materias:	Andean crops oca Oxalis rDNA RFLPs
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000300002
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spelling	oai:scielo:S0717-345820080003000022009-01-30Characterization of the nuclear ribosomal DNA unit in Oxalis tuberosa (Oxalidacea) and related speciesTosto,DanielaHopp,Esteban Andean crops oca Oxalis rDNA RFLPs Oxalis tuberosa is an octoploid Andean tuber crop called "oca" that belongs to the worldwide distributed genus Oxalis. The genus is very heterogeneous and its systematics is still problematic. It has been proposed that O. tuberosa evolved by polyploidization of a still not defined ancestor that belongs to an alliance of species sharing the same basic chromosome number (x = 8). Nuclear rDNA units of O. tuberosa and a selected group of four related diploid species were characterised by RFLP using different restriction endonucleases and southern hybridization probes to produce a restriction map for EcoRI and BamHI. The major rDNA unit length in O. tuberosa was estimated at 10.7 kbp. As expected, restriction site variation was observed mainly in the intergenic spacer (IGS), but was also detected in coding regions. Restriction site mapping organization of the transcribed rDNA unit of O. tuberosa is very similar to O. oblongiformis. Nucleotide sequencing of a region of O. peduncularis IGS generated a complex organization pattern of repeats and subrepeats. Diploid species O. peduncularis, O. tabaconasensis and O. aff. villosula exhibited a ladder pattern that is a consequence of a 170 bp subrepeat unit indicating that these species share organization similarity and sequence homology. The variation pattern provided information to compare among diploid species, although it did not help to clarify taxonomic relationships between O. tuberosa and the putative diploid ancestors analysed in this study. Nonetheless, the RFLP pattern exhibited by O. tuberosa for the IGS region was quite unique and will be a useful tool to prospect in other related species.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.11 n.3 20082008-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000300002en10.4067/S0717-34582008000300002
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Andean crops oca Oxalis rDNA RFLPs
spellingShingle	Andean crops oca Oxalis rDNA RFLPs Tosto,Daniela Hopp,Esteban Characterization of the nuclear ribosomal DNA unit in Oxalis tuberosa (Oxalidacea) and related species
description	Oxalis tuberosa is an octoploid Andean tuber crop called "oca" that belongs to the worldwide distributed genus Oxalis. The genus is very heterogeneous and its systematics is still problematic. It has been proposed that O. tuberosa evolved by polyploidization of a still not defined ancestor that belongs to an alliance of species sharing the same basic chromosome number (x = 8). Nuclear rDNA units of O. tuberosa and a selected group of four related diploid species were characterised by RFLP using different restriction endonucleases and southern hybridization probes to produce a restriction map for EcoRI and BamHI. The major rDNA unit length in O. tuberosa was estimated at 10.7 kbp. As expected, restriction site variation was observed mainly in the intergenic spacer (IGS), but was also detected in coding regions. Restriction site mapping organization of the transcribed rDNA unit of O. tuberosa is very similar to O. oblongiformis. Nucleotide sequencing of a region of O. peduncularis IGS generated a complex organization pattern of repeats and subrepeats. Diploid species O. peduncularis, O. tabaconasensis and O. aff. villosula exhibited a ladder pattern that is a consequence of a 170 bp subrepeat unit indicating that these species share organization similarity and sequence homology. The variation pattern provided information to compare among diploid species, although it did not help to clarify taxonomic relationships between O. tuberosa and the putative diploid ancestors analysed in this study. Nonetheless, the RFLP pattern exhibited by O. tuberosa for the IGS region was quite unique and will be a useful tool to prospect in other related species.
author	Tosto,Daniela Hopp,Esteban
author_facet	Tosto,Daniela Hopp,Esteban
author_sort	Tosto,Daniela
title	Characterization of the nuclear ribosomal DNA unit in Oxalis tuberosa (Oxalidacea) and related species
title_short	Characterization of the nuclear ribosomal DNA unit in Oxalis tuberosa (Oxalidacea) and related species
title_full	Characterization of the nuclear ribosomal DNA unit in Oxalis tuberosa (Oxalidacea) and related species
title_fullStr	Characterization of the nuclear ribosomal DNA unit in Oxalis tuberosa (Oxalidacea) and related species
title_full_unstemmed	Characterization of the nuclear ribosomal DNA unit in Oxalis tuberosa (Oxalidacea) and related species
title_sort	characterization of the nuclear ribosomal dna unit in oxalis tuberosa (oxalidacea) and related species
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2008
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000300002
work_keys_str_mv	AT tostodaniela characterizationofthenuclearribosomaldnaunitinoxalistuberosaoxalidaceaandrelatedspecies AT hoppesteban characterizationofthenuclearribosomaldnaunitinoxalistuberosaoxalidaceaandrelatedspecies
_version_	1718441785628295168

Characterization of the nuclear ribosomal DNA unit in Oxalis tuberosa (Oxalidacea) and related species

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