Development of transformation vectors for the production of potentially high oleate transgenic oil palm

The main target of Malaysian Palm Oil Board (MPOB) genetic engineering programme is to produce high oleate transgenic palms. The availability of effective transformation vector is one of the pre-requisites for genetic manipulation of oil palm through recombinant DNA technology. Here, we describe the...

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Autores principales:	Masani,Mat Yunus Abdul, Parveez,Ghulam Kadir Ahmad
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2008
Materias:	oleate transformation vectors transgenic oil palm
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000300003
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spelling	oai:scielo:S0717-345820080003000032009-01-30Development of transformation vectors for the production of potentially high oleate transgenic oil palmMasani,Mat Yunus AbdulParveez,Ghulam Kadir Ahmad oleate transformation vectors transgenic oil palm The main target of Malaysian Palm Oil Board (MPOB) genetic engineering programme is to produce high oleate transgenic palms. The availability of effective transformation vector is one of the pre-requisites for genetic manipulation of oil palm through recombinant DNA technology. Here, we describe the construction of a series of transformation vectors that have a maize ubiquitin promoter (UbiPro)-driven bar gene for selection of transformants on herbicide (Basta or Bialaphos), and mesocarp-specific promoter (MSP1) for expression of the transgenes [antisense palmitoyl-ACP-thioesterase (PAT) and sense β-ketoacyl-ACP-synthase II (KASII) and sense Δ9-stearoyl-ACP-desaturase (SAD)] potentially responsible for high oleate content in oil palm mesocarp. The transformation vectors constructed in this study are suitable for use in both particle bombardment (biolistic) and Agrobacterium-based transformation protocols.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.11 n.3 20082008-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000300003en10.4067/S0717-34582008000300003
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	oleate transformation vectors transgenic oil palm
spellingShingle	oleate transformation vectors transgenic oil palm Masani,Mat Yunus Abdul Parveez,Ghulam Kadir Ahmad Development of transformation vectors for the production of potentially high oleate transgenic oil palm
description	The main target of Malaysian Palm Oil Board (MPOB) genetic engineering programme is to produce high oleate transgenic palms. The availability of effective transformation vector is one of the pre-requisites for genetic manipulation of oil palm through recombinant DNA technology. Here, we describe the construction of a series of transformation vectors that have a maize ubiquitin promoter (UbiPro)-driven bar gene for selection of transformants on herbicide (Basta or Bialaphos), and mesocarp-specific promoter (MSP1) for expression of the transgenes [antisense palmitoyl-ACP-thioesterase (PAT) and sense β-ketoacyl-ACP-synthase II (KASII) and sense Δ9-stearoyl-ACP-desaturase (SAD)] potentially responsible for high oleate content in oil palm mesocarp. The transformation vectors constructed in this study are suitable for use in both particle bombardment (biolistic) and Agrobacterium-based transformation protocols.
author	Masani,Mat Yunus Abdul Parveez,Ghulam Kadir Ahmad
author_facet	Masani,Mat Yunus Abdul Parveez,Ghulam Kadir Ahmad
author_sort	Masani,Mat Yunus Abdul
title	Development of transformation vectors for the production of potentially high oleate transgenic oil palm
title_short	Development of transformation vectors for the production of potentially high oleate transgenic oil palm
title_full	Development of transformation vectors for the production of potentially high oleate transgenic oil palm
title_fullStr	Development of transformation vectors for the production of potentially high oleate transgenic oil palm
title_full_unstemmed	Development of transformation vectors for the production of potentially high oleate transgenic oil palm
title_sort	development of transformation vectors for the production of potentially high oleate transgenic oil palm
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2008
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000300003
work_keys_str_mv	AT masanimatyunusabdul developmentoftransformationvectorsfortheproductionofpotentiallyhigholeatetransgenicoilpalm AT parveezghulamkadirahmad developmentoftransformationvectorsfortheproductionofpotentiallyhigholeatetransgenicoilpalm
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Development of transformation vectors for the production of potentially high oleate transgenic oil palm

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