Efficiency of RAPD and ISSR markers system in accessing genetic variation of rice bean (Vigna umbellata) landraces

Vigna umbellata (Thunb.) Ohwi and Ohashi commonly known as rice bean or climbing mountain bean is under-exploited tropical legume. Genetic variation between 10 landraces of rice bean was evaluated using random amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) markers. Among t...

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Autores principales:	Muthusamy,Saraladevi, Kanagarajan,Selvaraju, Ponnusamy,Shanmugasundaram
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2008
Materias:	genetic diversity ISSRs landraces rice bean RAPDs Vigna umbellata
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000300004
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spelling	oai:scielo:S0717-345820080003000042009-01-30Efficiency of RAPD and ISSR markers system in accessing genetic variation of rice bean (Vigna umbellata) landracesMuthusamy,SaraladeviKanagarajan,SelvarajuPonnusamy,Shanmugasundaram genetic diversity ISSRs landraces rice bean RAPDs Vigna umbellata Vigna umbellata (Thunb.) Ohwi and Ohashi commonly known as rice bean or climbing mountain bean is under-exploited tropical legume. Genetic variation between 10 landraces of rice bean was evaluated using random amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) markers. Among these markers, RAPD primers generated 987 amplification products of which 719 were polymorphic and ISSR markers produced 479 amplification products, out of which 296 were polymorphic. RAPD fingerprinting detected more polymorphic loci (70.30%) than the ISSR fingerprinting (61.79%). Mean PIC (polymorphism information content) for each of these marker systems (0.243 for RAPD and 0.203 for ISSR) suggested that both the marker systems were equally effective in determining polymorphisms. The dendrograms constructed using RAPD and ISSR marker systems were highly correlated with each other as revealed by high Mantel correlation (r = 0.95). Pairwise similarity index values ranged from 0.530 to 0.782 (RAPD), 0.608 and 0.862 (ISSR) and 0.559 to 0.777 (RAPD and ISSR) and mean similarity index value of 0.677, 0.729 and 0.694 for RAPD, ISSR and combined data, respectively. RAPD and ISSR marker systems were found to be useful for the genetic diversity studies in V. umbellata and identify variation within landraces.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.11 n.3 20082008-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000300004en10.4067/S0717-34582008000300004
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	genetic diversity ISSRs landraces rice bean RAPDs Vigna umbellata
spellingShingle	genetic diversity ISSRs landraces rice bean RAPDs Vigna umbellata Muthusamy,Saraladevi Kanagarajan,Selvaraju Ponnusamy,Shanmugasundaram Efficiency of RAPD and ISSR markers system in accessing genetic variation of rice bean (Vigna umbellata) landraces
description	Vigna umbellata (Thunb.) Ohwi and Ohashi commonly known as rice bean or climbing mountain bean is under-exploited tropical legume. Genetic variation between 10 landraces of rice bean was evaluated using random amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) markers. Among these markers, RAPD primers generated 987 amplification products of which 719 were polymorphic and ISSR markers produced 479 amplification products, out of which 296 were polymorphic. RAPD fingerprinting detected more polymorphic loci (70.30%) than the ISSR fingerprinting (61.79%). Mean PIC (polymorphism information content) for each of these marker systems (0.243 for RAPD and 0.203 for ISSR) suggested that both the marker systems were equally effective in determining polymorphisms. The dendrograms constructed using RAPD and ISSR marker systems were highly correlated with each other as revealed by high Mantel correlation (r = 0.95). Pairwise similarity index values ranged from 0.530 to 0.782 (RAPD), 0.608 and 0.862 (ISSR) and 0.559 to 0.777 (RAPD and ISSR) and mean similarity index value of 0.677, 0.729 and 0.694 for RAPD, ISSR and combined data, respectively. RAPD and ISSR marker systems were found to be useful for the genetic diversity studies in V. umbellata and identify variation within landraces.
author	Muthusamy,Saraladevi Kanagarajan,Selvaraju Ponnusamy,Shanmugasundaram
author_facet	Muthusamy,Saraladevi Kanagarajan,Selvaraju Ponnusamy,Shanmugasundaram
author_sort	Muthusamy,Saraladevi
title	Efficiency of RAPD and ISSR markers system in accessing genetic variation of rice bean (Vigna umbellata) landraces
title_short	Efficiency of RAPD and ISSR markers system in accessing genetic variation of rice bean (Vigna umbellata) landraces
title_full	Efficiency of RAPD and ISSR markers system in accessing genetic variation of rice bean (Vigna umbellata) landraces
title_fullStr	Efficiency of RAPD and ISSR markers system in accessing genetic variation of rice bean (Vigna umbellata) landraces
title_full_unstemmed	Efficiency of RAPD and ISSR markers system in accessing genetic variation of rice bean (Vigna umbellata) landraces
title_sort	efficiency of rapd and issr markers system in accessing genetic variation of rice bean (vigna umbellata) landraces
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2008
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000300004
work_keys_str_mv	AT muthusamysaraladevi efficiencyofrapdandissrmarkerssysteminaccessinggeneticvariationofricebeanvignaumbellatalandraces AT kanagarajanselvaraju efficiencyofrapdandissrmarkerssysteminaccessinggeneticvariationofricebeanvignaumbellatalandraces AT ponnusamyshanmugasundaram efficiencyofrapdandissrmarkerssysteminaccessinggeneticvariationofricebeanvignaumbellatalandraces
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Efficiency of RAPD and ISSR markers system in accessing genetic variation of rice bean (Vigna umbellata) landraces

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