Efficient protocol for isolation of functional RNA from different grape tissue rich in polyphenols and polysaccharides for gene expression studies

Efficient protocol for isolation of functional RNA from different grape tissue rich in polyphenols and polysaccharides for gene expression studies

Extraction of RNA from plant tissue containing high levels of polyphenols and polysaccharides is tedious and difficult in grapes. Although several protocols have been published for plant RNA isolation, most have failed to yield high quality RNA in sufficient quantity from mature and diseased grape t...

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Autores principales: Vasanthaiah,Hemanth K.N., Katam,Ramesh, Sheikh,Mehboob B
Lenguaje:English
Publicado: Pontificia Universidad Católica de Valparaíso 2008
Materias:
differential display RT-PCR
grape
RNA isolation
RT-PCR
subtractive hybridization
Vitis
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000300005
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