PCR-based sensitive detection of the edible fungus Boletus edulis from rDNA ITS sequences

Identification of commercially important fungi, such as the valuable edible fungus Boletus edulis can be difficult considering visual or metabolic approaches. Based on phylogenetic analysis of the rDNA ITS sequence, a pair of specific primers was designed for differentiating B. edulis from other mus...

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Autores principales:	Lian,Bin, Zang,Jin-ping, Hou,Wei-guo, Yuan,Sheng, Smith,Donald L
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2008
Materias:	Boletus edulis detection edible fungi internal transcribed spacer PCR specific primers
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000300011
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spelling	oai:scielo:S0717-345820080003000112009-01-30PCR-based sensitive detection of the edible fungus Boletus edulis from rDNA ITS sequencesLian,BinZang,Jin-pingHou,Wei-guoYuan,ShengSmith,Donald L Boletus edulis detection edible fungi internal transcribed spacer PCR specific primers Identification of commercially important fungi, such as the valuable edible fungus Boletus edulis can be difficult considering visual or metabolic approaches. Based on phylogenetic analysis of the rDNA ITS sequence, a pair of specific primers was designed for differentiating B. edulis from other mushrooms by PCR. PCR was performed with total DNA as a template at an annealing temperature between 56-60ºC. Positive amplicons were obtained from B. edulis with all DNA templates from fruit bodies and cultured mycelium, but not from other fungal species at an annealing temperature of 60ºC. The result indicated that B. edulis could be clearly distinguished from other fungi by PCR, and there were no misidentifications under the reaction conditions used. The primers were also successfully employed to identify various tissues of B. edulis.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.11 n.3 20082008-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000300011en10.4067/S0717-34582008000300011
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Boletus edulis detection edible fungi internal transcribed spacer PCR specific primers
spellingShingle	Boletus edulis detection edible fungi internal transcribed spacer PCR specific primers Lian,Bin Zang,Jin-ping Hou,Wei-guo Yuan,Sheng Smith,Donald L PCR-based sensitive detection of the edible fungus Boletus edulis from rDNA ITS sequences
description	Identification of commercially important fungi, such as the valuable edible fungus Boletus edulis can be difficult considering visual or metabolic approaches. Based on phylogenetic analysis of the rDNA ITS sequence, a pair of specific primers was designed for differentiating B. edulis from other mushrooms by PCR. PCR was performed with total DNA as a template at an annealing temperature between 56-60ºC. Positive amplicons were obtained from B. edulis with all DNA templates from fruit bodies and cultured mycelium, but not from other fungal species at an annealing temperature of 60ºC. The result indicated that B. edulis could be clearly distinguished from other fungi by PCR, and there were no misidentifications under the reaction conditions used. The primers were also successfully employed to identify various tissues of B. edulis.
author	Lian,Bin Zang,Jin-ping Hou,Wei-guo Yuan,Sheng Smith,Donald L
author_facet	Lian,Bin Zang,Jin-ping Hou,Wei-guo Yuan,Sheng Smith,Donald L
author_sort	Lian,Bin
title	PCR-based sensitive detection of the edible fungus Boletus edulis from rDNA ITS sequences
title_short	PCR-based sensitive detection of the edible fungus Boletus edulis from rDNA ITS sequences
title_full	PCR-based sensitive detection of the edible fungus Boletus edulis from rDNA ITS sequences
title_fullStr	PCR-based sensitive detection of the edible fungus Boletus edulis from rDNA ITS sequences
title_full_unstemmed	PCR-based sensitive detection of the edible fungus Boletus edulis from rDNA ITS sequences
title_sort	pcr-based sensitive detection of the edible fungus boletus edulis from rdna its sequences
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2008
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000300011
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PCR-based sensitive detection of the edible fungus Boletus edulis from rDNA ITS sequences

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