Cloning and characterization of resistance gene analogs from under-exploited plant species

Genomic DNA sequences sharing homology with NBS region of resistance gene analogs were isolated and characterized from Pongamia glabra, Adenanthera pavonina, Clitoria ternatea and Solanum trilobatum using PCR based approach with primers designed from conserved regions of NBS domain. The presence of...

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Autores principales:	Thirumalaiandi,Ramasubramanian, Selvaraj,Michael Gomez, Rajasekaran,Raghu, Subbarayalu,Mohankumar
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2008
Materias:	Adenanthera pavonina Clitoria ternatea NBS-LRR Pongamia glabra resistance gene analogs solanum trilobatum
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000400004
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spelling	oai:scielo:S0717-345820080004000042009-06-16Cloning and characterization of resistance gene analogs from under-exploited plant speciesThirumalaiandi,RamasubramanianSelvaraj,Michael GomezRajasekaran,RaghuSubbarayalu,Mohankumar Adenanthera pavonina Clitoria ternatea NBS-LRR Pongamia glabra resistance gene analogs solanum trilobatum Genomic DNA sequences sharing homology with NBS region of resistance gene analogs were isolated and characterized from Pongamia glabra, Adenanthera pavonina, Clitoria ternatea and Solanum trilobatum using PCR based approach with primers designed from conserved regions of NBS domain. The presence of consensus motifs viz., kinase 1a, kinase 2, kinase 3a and hydrophobic domain provided evidence that the cloned sequences may belong to the NBS-LRR gene family. Conservation of tryptophan as the last residue of kinase-2 motif further confirms their position in non-TIR NBS-LRR family of resistance genes. The Resistance Gene Analogs (RGAs) cloned from P. glabra, A. pavonina, C. ternatea and S. trilobatum clustered together with well- characterized non-TIR-NBS-LRR genes leaving the TIR-NBS-LRR genes as a separate cluster in the average distance tree constructed based on BLOSUM62. All the four RGAs had high level of identity with NBS-LRR family of RGAs deposited in the GenBank. The extent of identity between the sequences at NBS region varied from 29% (P. glabra and S. trilobatum) to 78% (A. pavonina and C. ternatea), which indicates the diversity among the RGAs.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.11 n.4 20082008-10-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000400004en10.4067/S0717-34582008000400004
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Adenanthera pavonina Clitoria ternatea NBS-LRR Pongamia glabra resistance gene analogs solanum trilobatum
spellingShingle	Adenanthera pavonina Clitoria ternatea NBS-LRR Pongamia glabra resistance gene analogs solanum trilobatum Thirumalaiandi,Ramasubramanian Selvaraj,Michael Gomez Rajasekaran,Raghu Subbarayalu,Mohankumar Cloning and characterization of resistance gene analogs from under-exploited plant species
description	Genomic DNA sequences sharing homology with NBS region of resistance gene analogs were isolated and characterized from Pongamia glabra, Adenanthera pavonina, Clitoria ternatea and Solanum trilobatum using PCR based approach with primers designed from conserved regions of NBS domain. The presence of consensus motifs viz., kinase 1a, kinase 2, kinase 3a and hydrophobic domain provided evidence that the cloned sequences may belong to the NBS-LRR gene family. Conservation of tryptophan as the last residue of kinase-2 motif further confirms their position in non-TIR NBS-LRR family of resistance genes. The Resistance Gene Analogs (RGAs) cloned from P. glabra, A. pavonina, C. ternatea and S. trilobatum clustered together with well- characterized non-TIR-NBS-LRR genes leaving the TIR-NBS-LRR genes as a separate cluster in the average distance tree constructed based on BLOSUM62. All the four RGAs had high level of identity with NBS-LRR family of RGAs deposited in the GenBank. The extent of identity between the sequences at NBS region varied from 29% (P. glabra and S. trilobatum) to 78% (A. pavonina and C. ternatea), which indicates the diversity among the RGAs.
author	Thirumalaiandi,Ramasubramanian Selvaraj,Michael Gomez Rajasekaran,Raghu Subbarayalu,Mohankumar
author_facet	Thirumalaiandi,Ramasubramanian Selvaraj,Michael Gomez Rajasekaran,Raghu Subbarayalu,Mohankumar
author_sort	Thirumalaiandi,Ramasubramanian
title	Cloning and characterization of resistance gene analogs from under-exploited plant species
title_short	Cloning and characterization of resistance gene analogs from under-exploited plant species
title_full	Cloning and characterization of resistance gene analogs from under-exploited plant species
title_fullStr	Cloning and characterization of resistance gene analogs from under-exploited plant species
title_full_unstemmed	Cloning and characterization of resistance gene analogs from under-exploited plant species
title_sort	cloning and characterization of resistance gene analogs from under-exploited plant species
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2008
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000400004
work_keys_str_mv	AT thirumalaiandiramasubramanian cloningandcharacterizationofresistancegeneanalogsfromunderexploitedplantspecies AT selvarajmichaelgomez cloningandcharacterizationofresistancegeneanalogsfromunderexploitedplantspecies AT rajasekaranraghu cloningandcharacterizationofresistancegeneanalogsfromunderexploitedplantspecies AT subbarayalumohankumar cloningandcharacterizationofresistancegeneanalogsfromunderexploitedplantspecies
_version_	1718441789510123520

Cloning and characterization of resistance gene analogs from under-exploited plant species

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