Kinetic study on inducibility of polygalacturonases from Aspergillus flavipes FP-500

The aim of this work was to describe growth dynamics, substrate depletion and polygalacturonases production by Aspergillus flavipes FP-500 in batch cultures by means of unstructured models. The microorganism was cultivated on several mono- di- and poly- saccharides, and then the culture development...

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Autores principales:	Martínez-Trujillo,Aurora, Aranda,Juan S, Aguilar-Osorio,Guillermo
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2008
Materias:	constitutive enzymes inducible enzymes initial pH kinetic modeling pectinases
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000400008
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spelling	oai:scielo:S0717-345820080004000082009-06-16Kinetic study on inducibility of polygalacturonases from Aspergillus flavipes FP-500Martínez-Trujillo,AuroraAranda,Juan SAguilar-Osorio,Guillermo constitutive enzymes inducible enzymes initial pH kinetic modeling pectinases The aim of this work was to describe growth dynamics, substrate depletion and polygalacturonases production by Aspergillus flavipes FP-500 in batch cultures by means of unstructured models. The microorganism was cultivated on several mono- di- and poly- saccharides, and then the culture development modeled with Monod and Leudeking-Piret equations. The kinetic parameters related to the models (µmax, γx/s, α and β) were obtained by minimizing the quadratic residuals function with a simplex algorithm. An accurate description of experimental data was attained with the proposed models. Besides, modeling provided significant kinetic information on microbial degradation of complex substrates, such as the correlation between specific growth rate µmax and production yield α, suggesting that A. flavipes FP-500 polygalacturonases are actually constitutive, but also that there is a certain degree of induciblility in these enzymatic activities.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.11 n.4 20082008-10-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000400008en10.4067/S0717-34582008000400008
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	constitutive enzymes inducible enzymes initial pH kinetic modeling pectinases
spellingShingle	constitutive enzymes inducible enzymes initial pH kinetic modeling pectinases Martínez-Trujillo,Aurora Aranda,Juan S Aguilar-Osorio,Guillermo Kinetic study on inducibility of polygalacturonases from Aspergillus flavipes FP-500
description	The aim of this work was to describe growth dynamics, substrate depletion and polygalacturonases production by Aspergillus flavipes FP-500 in batch cultures by means of unstructured models. The microorganism was cultivated on several mono- di- and poly- saccharides, and then the culture development modeled with Monod and Leudeking-Piret equations. The kinetic parameters related to the models (µmax, γx/s, α and β) were obtained by minimizing the quadratic residuals function with a simplex algorithm. An accurate description of experimental data was attained with the proposed models. Besides, modeling provided significant kinetic information on microbial degradation of complex substrates, such as the correlation between specific growth rate µmax and production yield α, suggesting that A. flavipes FP-500 polygalacturonases are actually constitutive, but also that there is a certain degree of induciblility in these enzymatic activities.
author	Martínez-Trujillo,Aurora Aranda,Juan S Aguilar-Osorio,Guillermo
author_facet	Martínez-Trujillo,Aurora Aranda,Juan S Aguilar-Osorio,Guillermo
author_sort	Martínez-Trujillo,Aurora
title	Kinetic study on inducibility of polygalacturonases from Aspergillus flavipes FP-500
title_short	Kinetic study on inducibility of polygalacturonases from Aspergillus flavipes FP-500
title_full	Kinetic study on inducibility of polygalacturonases from Aspergillus flavipes FP-500
title_fullStr	Kinetic study on inducibility of polygalacturonases from Aspergillus flavipes FP-500
title_full_unstemmed	Kinetic study on inducibility of polygalacturonases from Aspergillus flavipes FP-500
title_sort	kinetic study on inducibility of polygalacturonases from aspergillus flavipes fp-500
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2008
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000400008
work_keys_str_mv	AT martineztrujilloaurora kineticstudyoninducibilityofpolygalacturonasesfromaspergillusflavipesfp500 AT arandajuans kineticstudyoninducibilityofpolygalacturonasesfromaspergillusflavipesfp500 AT aguilarosorioguillermo kineticstudyoninducibilityofpolygalacturonasesfromaspergillusflavipesfp500
_version_	1718441790461181952

Kinetic study on inducibility of polygalacturonases from Aspergillus flavipes FP-500

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