Optimization of b-galactosidase production using Kluyveromyces lactis NRRL Y-8279 by response surface methodology

This paper investigates the production and optimization of b-galactosidase enzyme using synthetic medium by Kluyveromyces lactis NRRL Y-8279 in shake flask cultures. Among the different cell disintegration methods used, the highest specific activity was obtained when the cells were permeabilized usi...

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Autores principales:	Dagbagli,Seval, Goksungur,Yekta
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2008
Materias:	b-galactosidase Kluyveromyces lactis response surface methodology shake flask culture synthetic medium
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000400011
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spelling	oai:scielo:S0717-345820080004000112009-06-16Optimization of b-galactosidase production using Kluyveromyces lactis NRRL Y-8279 by response surface methodologyDagbagli,SevalGoksungur,Yekta b-galactosidase Kluyveromyces lactis response surface methodology shake flask culture synthetic medium This paper investigates the production and optimization of b-galactosidase enzyme using synthetic medium by Kluyveromyces lactis NRRL Y-8279 in shake flask cultures. Among the different cell disintegration methods used, the highest specific activity was obtained when the cells were permeabilized using isoamyl alcohol. Response surface methodology was used to investigate the effects of four fermentation parameters (agitation speed, pH, initial substrate concentration and incubation time) on b-galactosidase enzyme production. Results of the statistical analysis showed that the fit of the model was good in all cases. Maximum specific enzyme activity of 4218.4 U g-1 was obtained at the optimum levels of process variables (pH 7.35, agitation speed 179.2 rpm, initial sugar concentration 24.9 g l-1 and incubation time 50.9 hrs). The response surface methodology was found to be useful in optimizing and determining the interactions among process variables in b-galactosidase enzyme production.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.11 n.4 20082008-10-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000400011en10.4067/S0717-34582008000400011
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	b-galactosidase Kluyveromyces lactis response surface methodology shake flask culture synthetic medium
spellingShingle	b-galactosidase Kluyveromyces lactis response surface methodology shake flask culture synthetic medium Dagbagli,Seval Goksungur,Yekta Optimization of b-galactosidase production using Kluyveromyces lactis NRRL Y-8279 by response surface methodology
description	This paper investigates the production and optimization of b-galactosidase enzyme using synthetic medium by Kluyveromyces lactis NRRL Y-8279 in shake flask cultures. Among the different cell disintegration methods used, the highest specific activity was obtained when the cells were permeabilized using isoamyl alcohol. Response surface methodology was used to investigate the effects of four fermentation parameters (agitation speed, pH, initial substrate concentration and incubation time) on b-galactosidase enzyme production. Results of the statistical analysis showed that the fit of the model was good in all cases. Maximum specific enzyme activity of 4218.4 U g-1 was obtained at the optimum levels of process variables (pH 7.35, agitation speed 179.2 rpm, initial sugar concentration 24.9 g l-1 and incubation time 50.9 hrs). The response surface methodology was found to be useful in optimizing and determining the interactions among process variables in b-galactosidase enzyme production.
author	Dagbagli,Seval Goksungur,Yekta
author_facet	Dagbagli,Seval Goksungur,Yekta
author_sort	Dagbagli,Seval
title	Optimization of b-galactosidase production using Kluyveromyces lactis NRRL Y-8279 by response surface methodology
title_short	Optimization of b-galactosidase production using Kluyveromyces lactis NRRL Y-8279 by response surface methodology
title_full	Optimization of b-galactosidase production using Kluyveromyces lactis NRRL Y-8279 by response surface methodology
title_fullStr	Optimization of b-galactosidase production using Kluyveromyces lactis NRRL Y-8279 by response surface methodology
title_full_unstemmed	Optimization of b-galactosidase production using Kluyveromyces lactis NRRL Y-8279 by response surface methodology
title_sort	optimization of b-galactosidase production using kluyveromyces lactis nrrl y-8279 by response surface methodology
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2008
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000400011
work_keys_str_mv	AT dagbagliseval optimizationofbgalactosidaseproductionusingkluyveromyceslactisnrrly8279byresponsesurfacemethodology AT goksunguryekta optimizationofbgalactosidaseproductionusingkluyveromyceslactisnrrly8279byresponsesurfacemethodology
_version_	1718441791154290688

Optimization of b-galactosidase production using Kluyveromyces lactis NRRL Y-8279 by response surface methodology

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