Assessment of genetic diversity in Venezuelan rice cultivars using simple sequence repeats markers

In Venezuela, pedigree analyses indicate that the rice varieties currently under cultivation are closely related. Effective breeding programs, based on knowledge of the genetic diversity of cultivars, are needed to broaden the genetic bases of rice germplasm in the country. In this study, we used a...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ghneim Herrera,Thaura, Posso Duque,Duina, Pérez Almeida,Iris, Torrealba Núñez,Gelis, Pieters,Alejandro J, Martinez,César P, Tohme,Joe M
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2008
Materias:	DNA fingerprinting genetic diversity rice SSR
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000500003
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0717-34582008000500003
record_format	dspace
spelling	oai:scielo:S0717-345820080005000032009-06-16Assessment of genetic diversity in Venezuelan rice cultivars using simple sequence repeats markersGhneim Herrera,ThauraPosso Duque,DuinaPérez Almeida,IrisTorrealba Núñez,GelisPieters,Alejandro JMartinez,César PTohme,Joe M DNA fingerprinting genetic diversity rice SSR In Venezuela, pedigree analyses indicate that the rice varieties currently under cultivation are closely related. Effective breeding programs, based on knowledge of the genetic diversity of cultivars, are needed to broaden the genetic bases of rice germplasm in the country. In this study, we used a set of 48 simple-sequence-repeat (SSR) markers to assess the genetic diversity of 11 Venezuelan rice cultivars, released by the National Rice Breeding Program between 1978 and 2007. A total of 203 alleles were detected, the number of alleles (NA) per marker ranged from 2 to 9, with an average of 4.23. The average genic diversity (H) over all SSR loci for the 18 genotypes was 0.524, ranging from 0.105 to 0.815. Positive correlations were found between H at each locus, NA, the allele size range and the maximum number of repeats. Venezuelan cultivars showed lower H (mean = 0.37) and NA (total = 124, mean = 2.58) than the whole sample. UPGMA-cluster-analysis based on genetic distance coefficients clearly separated all the genotypes, and showed that the Venezuelan rice varieties are closely related. Molecular identification of 7 Venezuelan cultivars could be done with 9 primers pairs which produced 10 genotype-specific-alleles. Although the genetic diversity was low, SSRs proved to be an efficient tool in assessing the genetic diversity of rice genotypes. Implications of the low genetic diversity detected and relatedness of Venezuelan cultivars are discussed.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.11 n.5 20082008-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000500003en10.4067/S0717-34582008000500003
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	DNA fingerprinting genetic diversity rice SSR
spellingShingle	DNA fingerprinting genetic diversity rice SSR Ghneim Herrera,Thaura Posso Duque,Duina Pérez Almeida,Iris Torrealba Núñez,Gelis Pieters,Alejandro J Martinez,César P Tohme,Joe M Assessment of genetic diversity in Venezuelan rice cultivars using simple sequence repeats markers
description	In Venezuela, pedigree analyses indicate that the rice varieties currently under cultivation are closely related. Effective breeding programs, based on knowledge of the genetic diversity of cultivars, are needed to broaden the genetic bases of rice germplasm in the country. In this study, we used a set of 48 simple-sequence-repeat (SSR) markers to assess the genetic diversity of 11 Venezuelan rice cultivars, released by the National Rice Breeding Program between 1978 and 2007. A total of 203 alleles were detected, the number of alleles (NA) per marker ranged from 2 to 9, with an average of 4.23. The average genic diversity (H) over all SSR loci for the 18 genotypes was 0.524, ranging from 0.105 to 0.815. Positive correlations were found between H at each locus, NA, the allele size range and the maximum number of repeats. Venezuelan cultivars showed lower H (mean = 0.37) and NA (total = 124, mean = 2.58) than the whole sample. UPGMA-cluster-analysis based on genetic distance coefficients clearly separated all the genotypes, and showed that the Venezuelan rice varieties are closely related. Molecular identification of 7 Venezuelan cultivars could be done with 9 primers pairs which produced 10 genotype-specific-alleles. Although the genetic diversity was low, SSRs proved to be an efficient tool in assessing the genetic diversity of rice genotypes. Implications of the low genetic diversity detected and relatedness of Venezuelan cultivars are discussed.
author	Ghneim Herrera,Thaura Posso Duque,Duina Pérez Almeida,Iris Torrealba Núñez,Gelis Pieters,Alejandro J Martinez,César P Tohme,Joe M
author_facet	Ghneim Herrera,Thaura Posso Duque,Duina Pérez Almeida,Iris Torrealba Núñez,Gelis Pieters,Alejandro J Martinez,César P Tohme,Joe M
author_sort	Ghneim Herrera,Thaura
title	Assessment of genetic diversity in Venezuelan rice cultivars using simple sequence repeats markers
title_short	Assessment of genetic diversity in Venezuelan rice cultivars using simple sequence repeats markers
title_full	Assessment of genetic diversity in Venezuelan rice cultivars using simple sequence repeats markers
title_fullStr	Assessment of genetic diversity in Venezuelan rice cultivars using simple sequence repeats markers
title_full_unstemmed	Assessment of genetic diversity in Venezuelan rice cultivars using simple sequence repeats markers
title_sort	assessment of genetic diversity in venezuelan rice cultivars using simple sequence repeats markers
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2008
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000500003
work_keys_str_mv	AT ghneimherrerathaura assessmentofgeneticdiversityinvenezuelanricecultivarsusingsimplesequencerepeatsmarkers AT possoduqueduina assessmentofgeneticdiversityinvenezuelanricecultivarsusingsimplesequencerepeatsmarkers AT perezalmeidairis assessmentofgeneticdiversityinvenezuelanricecultivarsusingsimplesequencerepeatsmarkers AT torrealbanunezgelis assessmentofgeneticdiversityinvenezuelanricecultivarsusingsimplesequencerepeatsmarkers AT pietersalejandroj assessmentofgeneticdiversityinvenezuelanricecultivarsusingsimplesequencerepeatsmarkers AT martinezcesarp assessmentofgeneticdiversityinvenezuelanricecultivarsusingsimplesequencerepeatsmarkers AT tohmejoem assessmentofgeneticdiversityinvenezuelanricecultivarsusingsimplesequencerepeatsmarkers
_version_	1718441793353154560

Assessment of genetic diversity in Venezuelan rice cultivars using simple sequence repeats markers

Ejemplares similares