Identification of a minimal microsatellite marker panel for the fingerprinting of peach and nectarine cultivars

The genetic characterization of 117 peach and nectarine cultivars (Prunus persica (L.) Batsch) using microsatellite (SSR) markers is presented. Analyzed genotypes include the complete list of cultivars under intellectual property (IP) protection in Chile. One hundred and two out of the 117 cultivars...

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Autores principales:	Rojas,Gabriela, Méndez,Marco A, Muñoz,Carlos, Lemus,Gamalier, Hinrichsen,Patricio
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2008
Materias:	cultivar identification fingerprinting Prunus persica simple sequence repeats SSR
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000500004
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spelling	oai:scielo:S0717-345820080005000042009-06-16Identification of a minimal microsatellite marker panel for the fingerprinting of peach and nectarine cultivarsRojas,GabrielaMéndez,Marco AMuñoz,CarlosLemus,GamalierHinrichsen,Patricio cultivar identification fingerprinting Prunus persica simple sequence repeats SSR The genetic characterization of 117 peach and nectarine cultivars (Prunus persica (L.) Batsch) using microsatellite (SSR) markers is presented. Analyzed genotypes include the complete list of cultivars under intellectual property (IP) protection in Chile. One hundred and two out of the 117 cultivars under study could be identified using only 7 SSRs. Other 5 cultivars were differentiated using 3 additional markers, but 5 pairs of genotypes were not differentiable. The average expected heterozygosity for the set of markers was 0.55, ranging from 0.28 in BPPCT-008 to 0.81 in CPPCT-022, with an F value of 0.37. A Neighbor-Joining dendrogram showed that, with few exceptions, peaches and nectarines clustered separately. These results are the basis for the development of a fingerprinting protocol for the unequivocal identification of most of the peach and nectarine cultivars officially registered in Chile.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.11 n.5 20082008-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000500004en10.4067/S0717-34582008000500004
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	cultivar identification fingerprinting Prunus persica simple sequence repeats SSR
spellingShingle	cultivar identification fingerprinting Prunus persica simple sequence repeats SSR Rojas,Gabriela Méndez,Marco A Muñoz,Carlos Lemus,Gamalier Hinrichsen,Patricio Identification of a minimal microsatellite marker panel for the fingerprinting of peach and nectarine cultivars
description	The genetic characterization of 117 peach and nectarine cultivars (Prunus persica (L.) Batsch) using microsatellite (SSR) markers is presented. Analyzed genotypes include the complete list of cultivars under intellectual property (IP) protection in Chile. One hundred and two out of the 117 cultivars under study could be identified using only 7 SSRs. Other 5 cultivars were differentiated using 3 additional markers, but 5 pairs of genotypes were not differentiable. The average expected heterozygosity for the set of markers was 0.55, ranging from 0.28 in BPPCT-008 to 0.81 in CPPCT-022, with an F value of 0.37. A Neighbor-Joining dendrogram showed that, with few exceptions, peaches and nectarines clustered separately. These results are the basis for the development of a fingerprinting protocol for the unequivocal identification of most of the peach and nectarine cultivars officially registered in Chile.
author	Rojas,Gabriela Méndez,Marco A Muñoz,Carlos Lemus,Gamalier Hinrichsen,Patricio
author_facet	Rojas,Gabriela Méndez,Marco A Muñoz,Carlos Lemus,Gamalier Hinrichsen,Patricio
author_sort	Rojas,Gabriela
title	Identification of a minimal microsatellite marker panel for the fingerprinting of peach and nectarine cultivars
title_short	Identification of a minimal microsatellite marker panel for the fingerprinting of peach and nectarine cultivars
title_full	Identification of a minimal microsatellite marker panel for the fingerprinting of peach and nectarine cultivars
title_fullStr	Identification of a minimal microsatellite marker panel for the fingerprinting of peach and nectarine cultivars
title_full_unstemmed	Identification of a minimal microsatellite marker panel for the fingerprinting of peach and nectarine cultivars
title_sort	identification of a minimal microsatellite marker panel for the fingerprinting of peach and nectarine cultivars
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2008
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000500004
work_keys_str_mv	AT rojasgabriela identificationofaminimalmicrosatellitemarkerpanelforthefingerprintingofpeachandnectarinecultivars AT mendezmarcoa identificationofaminimalmicrosatellitemarkerpanelforthefingerprintingofpeachandnectarinecultivars AT munozcarlos identificationofaminimalmicrosatellitemarkerpanelforthefingerprintingofpeachandnectarinecultivars AT lemusgamalier identificationofaminimalmicrosatellitemarkerpanelforthefingerprintingofpeachandnectarinecultivars AT hinrichsenpatricio identificationofaminimalmicrosatellitemarkerpanelforthefingerprintingofpeachandnectarinecultivars
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Identification of a minimal microsatellite marker panel for the fingerprinting of peach and nectarine cultivars

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