Novel s-triazine-degrading bacteria isolated from agricultural soils of central Chile for herbicide bioremediation

s-Triazine-degrading bacterial strains were isolated from long-term simazine-treated agricultural soils of central Chile. The number of culturable heterotrophic bacteria of these agricultural soils (7 x 10(6) CFU/g of dry soil) was not affected by simazine application on field. The simazine-degradin...

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Autores principales:	Hernández García,Marcela, Morgante,Verónica, Ávila Perez,Marcela, Villalobos Biaggini,Patricio, Miralles Noé,Pola, González Vergara,Myriam, Seeger Pfeiffer,Michael
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2008
Materias:	Arthrobacter biodegradation bioremediation simazine Stenotrophomonas s-triazine
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000500005
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spelling	oai:scielo:S0717-345820080005000052009-06-16Novel s-triazine-degrading bacteria isolated from agricultural soils of central Chile for herbicide bioremediationHernández García,MarcelaMorgante,VerónicaÁvila Perez,MarcelaVillalobos Biaggini,PatricioMiralles Noé,PolaGonzález Vergara,MyriamSeeger Pfeiffer,Michael Arthrobacter biodegradation bioremediation simazine Stenotrophomonas s-triazine s-Triazine-degrading bacterial strains were isolated from long-term simazine-treated agricultural soils of central Chile. The number of culturable heterotrophic bacteria of these agricultural soils (7 x 10(6) CFU/g of dry soil) was not affected by simazine application on field. The simazine-degrading bacterial strains P51, P52 and C53 were isolated by enrichment in minimal medium using simazine as the sole nitrogen source. Resting cells of strains P51 and P52 degraded >80% of simazine within 48 hrs, whereas strain C53 was able to remove >60% of the herbicide. The atzA and atzD genes of the s-triazine upper and lower catabolic pathways were detected in strains P51 and C53, while only atzD gene was observed in strain P52. To compare the bacterial 16S rRNA gene sequence structure, ARDRA were performed using the restriction enzymes Msp1 and Hha1. ARDRA indicated that strain P52 was a different ribotype than C53 and P51 strains. For further characterization the novel isolates were identified by 16S rRNA gene sequencing. Strains C53 and P51 belong to the genus Stenotrophomonas and the strain P52 belongs to the genus Arthrobacter . s -Triazine-degrading bacterial strains isolated from contaminated soils could be used as biocatalysts for bioremediation of these herbicides.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.11 n.5 20082008-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000500005en10.4067/S0717-34582008000500005
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Arthrobacter biodegradation bioremediation simazine Stenotrophomonas s-triazine
spellingShingle	Arthrobacter biodegradation bioremediation simazine Stenotrophomonas s-triazine Hernández García,Marcela Morgante,Verónica Ávila Perez,Marcela Villalobos Biaggini,Patricio Miralles Noé,Pola González Vergara,Myriam Seeger Pfeiffer,Michael Novel s-triazine-degrading bacteria isolated from agricultural soils of central Chile for herbicide bioremediation
description	s-Triazine-degrading bacterial strains were isolated from long-term simazine-treated agricultural soils of central Chile. The number of culturable heterotrophic bacteria of these agricultural soils (7 x 10(6) CFU/g of dry soil) was not affected by simazine application on field. The simazine-degrading bacterial strains P51, P52 and C53 were isolated by enrichment in minimal medium using simazine as the sole nitrogen source. Resting cells of strains P51 and P52 degraded >80% of simazine within 48 hrs, whereas strain C53 was able to remove >60% of the herbicide. The atzA and atzD genes of the s-triazine upper and lower catabolic pathways were detected in strains P51 and C53, while only atzD gene was observed in strain P52. To compare the bacterial 16S rRNA gene sequence structure, ARDRA were performed using the restriction enzymes Msp1 and Hha1. ARDRA indicated that strain P52 was a different ribotype than C53 and P51 strains. For further characterization the novel isolates were identified by 16S rRNA gene sequencing. Strains C53 and P51 belong to the genus Stenotrophomonas and the strain P52 belongs to the genus Arthrobacter . s -Triazine-degrading bacterial strains isolated from contaminated soils could be used as biocatalysts for bioremediation of these herbicides.
author	Hernández García,Marcela Morgante,Verónica Ávila Perez,Marcela Villalobos Biaggini,Patricio Miralles Noé,Pola González Vergara,Myriam Seeger Pfeiffer,Michael
author_facet	Hernández García,Marcela Morgante,Verónica Ávila Perez,Marcela Villalobos Biaggini,Patricio Miralles Noé,Pola González Vergara,Myriam Seeger Pfeiffer,Michael
author_sort	Hernández García,Marcela
title	Novel s-triazine-degrading bacteria isolated from agricultural soils of central Chile for herbicide bioremediation
title_short	Novel s-triazine-degrading bacteria isolated from agricultural soils of central Chile for herbicide bioremediation
title_full	Novel s-triazine-degrading bacteria isolated from agricultural soils of central Chile for herbicide bioremediation
title_fullStr	Novel s-triazine-degrading bacteria isolated from agricultural soils of central Chile for herbicide bioremediation
title_full_unstemmed	Novel s-triazine-degrading bacteria isolated from agricultural soils of central Chile for herbicide bioremediation
title_sort	novel s-triazine-degrading bacteria isolated from agricultural soils of central chile for herbicide bioremediation
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2008
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000500005
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Novel s-triazine-degrading bacteria isolated from agricultural soils of central Chile for herbicide bioremediation

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