Obtainment of embryogenic cell suspensions from scalps of the banana CIEN-BTA-03 (Musa sp., AAAA) and regeneration of the plants

The purposes of this work were to obtain embryogenic cell suspensions (ECS) from scalps and to regenerate plants of the banana CIEN-BTA-03. Shoot apexes were grown in the scalp-induction medium of Murashige and Skoog plus BA and IAA, following four diverse treatments. The first two, ME22 and ME25, w...

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Autores principales:	Ramírez-Villalobos,Maribel, de García,Eva
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2008
Materias:	bananas embryogenic callus embryogenic cell suspension Musa sp plant regeneration scalp secondary somatic embryogenesis
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000500006
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spelling	oai:scielo:S0717-345820080005000062009-06-16Obtainment of embryogenic cell suspensions from scalps of the banana CIEN-BTA-03 (Musa sp., AAAA) and regeneration of the plantsRamírez-Villalobos,Maribelde García,Eva bananas embryogenic callus embryogenic cell suspension Musa sp plant regeneration scalp secondary somatic embryogenesis The purposes of this work were to obtain embryogenic cell suspensions (ECS) from scalps and to regenerate plants of the banana CIEN-BTA-03. Shoot apexes were grown in the scalp-induction medium of Murashige and Skoog plus BA and IAA, following four diverse treatments. The first two, ME22 and ME25, were solid media supplemented with (mg L-1) 22.7 BA plus 0.192 IAA, and 25 BA plus 0.217 IAA, respectively, all containing 1.8 g L-1 of phytagel, and subcultures were performed monthly and bimonthly over 16 months. The other two treatments, IT22 and IT25, resembled ME22 and ME25 but consisted in temporary immersion for four months without subcultures, followed by two months in solid media. The scalps were grown in callus-induction medium and embryogenic calluses were obtained with abundant somatic embryos, especially in scalps from IT25. About 10 to 15 embryos from each were transferred to 5 ml of multiplication medium to initiate the ECS. The scalps obtained from the IT25 treatment were the most successful as they led to ECS with high embryogenic capability. In addition, IT25 decreased the timespan required for the production of scalps. The obtained ECS gave rise to secondary somatic embryos. It showed a high multiplication index, as well as numerous mature somatic embryos, and good conversion of embryos and plant regeneration.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.11 n.5 20082008-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000500006en10.4067/S0717-34582008000500006
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	bananas embryogenic callus embryogenic cell suspension Musa sp plant regeneration scalp secondary somatic embryogenesis
spellingShingle	bananas embryogenic callus embryogenic cell suspension Musa sp plant regeneration scalp secondary somatic embryogenesis Ramírez-Villalobos,Maribel de García,Eva Obtainment of embryogenic cell suspensions from scalps of the banana CIEN-BTA-03 (Musa sp., AAAA) and regeneration of the plants
description	The purposes of this work were to obtain embryogenic cell suspensions (ECS) from scalps and to regenerate plants of the banana CIEN-BTA-03. Shoot apexes were grown in the scalp-induction medium of Murashige and Skoog plus BA and IAA, following four diverse treatments. The first two, ME22 and ME25, were solid media supplemented with (mg L-1) 22.7 BA plus 0.192 IAA, and 25 BA plus 0.217 IAA, respectively, all containing 1.8 g L-1 of phytagel, and subcultures were performed monthly and bimonthly over 16 months. The other two treatments, IT22 and IT25, resembled ME22 and ME25 but consisted in temporary immersion for four months without subcultures, followed by two months in solid media. The scalps were grown in callus-induction medium and embryogenic calluses were obtained with abundant somatic embryos, especially in scalps from IT25. About 10 to 15 embryos from each were transferred to 5 ml of multiplication medium to initiate the ECS. The scalps obtained from the IT25 treatment were the most successful as they led to ECS with high embryogenic capability. In addition, IT25 decreased the timespan required for the production of scalps. The obtained ECS gave rise to secondary somatic embryos. It showed a high multiplication index, as well as numerous mature somatic embryos, and good conversion of embryos and plant regeneration.
author	Ramírez-Villalobos,Maribel de García,Eva
author_facet	Ramírez-Villalobos,Maribel de García,Eva
author_sort	Ramírez-Villalobos,Maribel
title	Obtainment of embryogenic cell suspensions from scalps of the banana CIEN-BTA-03 (Musa sp., AAAA) and regeneration of the plants
title_short	Obtainment of embryogenic cell suspensions from scalps of the banana CIEN-BTA-03 (Musa sp., AAAA) and regeneration of the plants
title_full	Obtainment of embryogenic cell suspensions from scalps of the banana CIEN-BTA-03 (Musa sp., AAAA) and regeneration of the plants
title_fullStr	Obtainment of embryogenic cell suspensions from scalps of the banana CIEN-BTA-03 (Musa sp., AAAA) and regeneration of the plants
title_full_unstemmed	Obtainment of embryogenic cell suspensions from scalps of the banana CIEN-BTA-03 (Musa sp., AAAA) and regeneration of the plants
title_sort	obtainment of embryogenic cell suspensions from scalps of the banana cien-bta-03 (musa sp., aaaa) and regeneration of the plants
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2008
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000500006
work_keys_str_mv	AT ramirezvillalobosmaribel obtainmentofembryogeniccellsuspensionsfromscalpsofthebananacienbta03musaspaaaaandregenerationoftheplants AT degarciaeva obtainmentofembryogeniccellsuspensionsfromscalpsofthebananacienbta03musaspaaaaandregenerationoftheplants
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Obtainment of embryogenic cell suspensions from scalps of the banana CIEN-BTA-03 (Musa sp., AAAA) and regeneration of the plants

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