Genetic variability for waxy genes in Argentinean bread wheat germplasm

Amylose and amylopectin are the two polysaccharides that constitute starch in bread wheat and the enzyme GBSSI (Granule-bound starch synthase I), also known as waxy protein, is responsible for amylose synthesis in storage tissues. Decrease of the amylose content in starch has been associated with th...

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Autores principales:	Vanzetti,Leonardo Sebastián, Pflüger,Laura Alicia, Rodríguez-Quijano,Marta, Carrillo,José Maria, Helguera,Marcelo
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2009
Materias:	characterization molecular markers Triticum aestivum L. starch waxy genes wheat
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000100004
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spelling	oai:scielo:S0717-345820090001000042009-07-29Genetic variability for waxy genes in Argentinean bread wheat germplasmVanzetti,Leonardo SebastiánPflüger,Laura AliciaRodríguez-Quijano,MartaCarrillo,José MariaHelguera,Marcelo characterization molecular markers Triticum aestivum L. starch waxy genes wheat Amylose and amylopectin are the two polysaccharides that constitute starch in bread wheat and the enzyme GBSSI (Granule-bound starch synthase I), also known as waxy protein, is responsible for amylose synthesis in storage tissues. Decrease of the amylose content in starch has been associated with the lack of waxy protein(s). In this work, different sets of PCR markers were used to characterize the genetic variability of waxy loci from 103 Argentinean bread wheat cultivars. For the Wx-A1 locus, Wx-A1a and a novel molecular allele designed Wx-A1g were detected. Wx-B1 locus showed three alleles (Wx-B1a, Wx-B1b, Wx-B1e), and Wx-D1 locus showed only the Wx-D1a allele. Novel single-locus allele specific markers for Wx-A1b, Wx-B1b and Wx-D1b null alleles were also described. To our best knowledge this is the first study focused to characterize the genetic variability for waxy genes in bread wheat cultivars from South America.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.12 n.1 20092009-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000100004en10.4067/S0717-34582009000100004
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	characterization molecular markers Triticum aestivum L. starch waxy genes wheat
spellingShingle	characterization molecular markers Triticum aestivum L. starch waxy genes wheat Vanzetti,Leonardo Sebastián Pflüger,Laura Alicia Rodríguez-Quijano,Marta Carrillo,José Maria Helguera,Marcelo Genetic variability for waxy genes in Argentinean bread wheat germplasm
description	Amylose and amylopectin are the two polysaccharides that constitute starch in bread wheat and the enzyme GBSSI (Granule-bound starch synthase I), also known as waxy protein, is responsible for amylose synthesis in storage tissues. Decrease of the amylose content in starch has been associated with the lack of waxy protein(s). In this work, different sets of PCR markers were used to characterize the genetic variability of waxy loci from 103 Argentinean bread wheat cultivars. For the Wx-A1 locus, Wx-A1a and a novel molecular allele designed Wx-A1g were detected. Wx-B1 locus showed three alleles (Wx-B1a, Wx-B1b, Wx-B1e), and Wx-D1 locus showed only the Wx-D1a allele. Novel single-locus allele specific markers for Wx-A1b, Wx-B1b and Wx-D1b null alleles were also described. To our best knowledge this is the first study focused to characterize the genetic variability for waxy genes in bread wheat cultivars from South America.
author	Vanzetti,Leonardo Sebastián Pflüger,Laura Alicia Rodríguez-Quijano,Marta Carrillo,José Maria Helguera,Marcelo
author_facet	Vanzetti,Leonardo Sebastián Pflüger,Laura Alicia Rodríguez-Quijano,Marta Carrillo,José Maria Helguera,Marcelo
author_sort	Vanzetti,Leonardo Sebastián
title	Genetic variability for waxy genes in Argentinean bread wheat germplasm
title_short	Genetic variability for waxy genes in Argentinean bread wheat germplasm
title_full	Genetic variability for waxy genes in Argentinean bread wheat germplasm
title_fullStr	Genetic variability for waxy genes in Argentinean bread wheat germplasm
title_full_unstemmed	Genetic variability for waxy genes in Argentinean bread wheat germplasm
title_sort	genetic variability for waxy genes in argentinean bread wheat germplasm
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2009
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000100004
work_keys_str_mv	AT vanzettileonardosebastian geneticvariabilityforwaxygenesinargentineanbreadwheatgermplasm AT pflugerlauraalicia geneticvariabilityforwaxygenesinargentineanbreadwheatgermplasm AT rodriguezquijanomarta geneticvariabilityforwaxygenesinargentineanbreadwheatgermplasm AT carrillojosemaria geneticvariabilityforwaxygenesinargentineanbreadwheatgermplasm AT helgueramarcelo geneticvariabilityforwaxygenesinargentineanbreadwheatgermplasm
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Genetic variability for waxy genes in Argentinean bread wheat germplasm

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