RAPD analysis of seized marijuana (Cannabis sativa L.) in Turkey

Cannabis sativa L. is a multiple-use plant. However, its cultivation is strictly controlled due to its psychoactive nature and usage in producing drugs such as marijuana, and hashish. In this study, psychoactive type Cannabis samples, which were seized from 29 different locations of Turkey, were use...

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Autores principales:	Pinarkara,Emine, Kayis,Seyit A, Hakki,Erdogan E, Sag,Ayla
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2009
Materias:	AMOVA cluster analysis individualization of seized marijuana principle coordinate analysis randomly amplified polymorphic DNA UPGMA
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000100005
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spelling	oai:scielo:S0717-345820090001000052009-07-29RAPD analysis of seized marijuana (Cannabis sativa L.) in TurkeyPinarkara,EmineKayis,Seyit AHakki,Erdogan ESag,Ayla AMOVA cluster analysis individualization of seized marijuana principle coordinate analysis randomly amplified polymorphic DNA UPGMA Cannabis sativa L. is a multiple-use plant. However, its cultivation is strictly controlled due to its psychoactive nature and usage in producing drugs such as marijuana, and hashish. In this study, psychoactive type Cannabis samples, which were seized from 29 different locations of Turkey, were used. Interests were to identify the genetic relatedness of the seized samples and to partition molecular variance between and within populations. Randomly Amplified Polymorphic DNAs were employed for analysis based on single plant material and bulked samples of them. Data were analysed via cluster and principal coordinate analyses (PCoA). Analysis of molecular variance (AMOVA) was performed to obtain variations between and within populations. Cannabis accessions were basically separated into two main groups by PCoA and cluster analyses according to geographical regions. One of them was made up of Cannabis plants, which were seized from mostly western part of Turkey (group 1). The other one was made up of Cannabis plants that were seized from mostly eastern part of Turkey (group 2). It is found that 20.23% of the genetic variation is due to differences between accessions groups while 79.77% of the genetic variation is due to between accessions within accessions groups. Compared to group1, group 2 showed more variation.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.12 n.1 20092009-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000100005en10.4067/S0717-34582009000100005
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	AMOVA cluster analysis individualization of seized marijuana principle coordinate analysis randomly amplified polymorphic DNA UPGMA
spellingShingle	AMOVA cluster analysis individualization of seized marijuana principle coordinate analysis randomly amplified polymorphic DNA UPGMA Pinarkara,Emine Kayis,Seyit A Hakki,Erdogan E Sag,Ayla RAPD analysis of seized marijuana (Cannabis sativa L.) in Turkey
description	Cannabis sativa L. is a multiple-use plant. However, its cultivation is strictly controlled due to its psychoactive nature and usage in producing drugs such as marijuana, and hashish. In this study, psychoactive type Cannabis samples, which were seized from 29 different locations of Turkey, were used. Interests were to identify the genetic relatedness of the seized samples and to partition molecular variance between and within populations. Randomly Amplified Polymorphic DNAs were employed for analysis based on single plant material and bulked samples of them. Data were analysed via cluster and principal coordinate analyses (PCoA). Analysis of molecular variance (AMOVA) was performed to obtain variations between and within populations. Cannabis accessions were basically separated into two main groups by PCoA and cluster analyses according to geographical regions. One of them was made up of Cannabis plants, which were seized from mostly western part of Turkey (group 1). The other one was made up of Cannabis plants that were seized from mostly eastern part of Turkey (group 2). It is found that 20.23% of the genetic variation is due to differences between accessions groups while 79.77% of the genetic variation is due to between accessions within accessions groups. Compared to group1, group 2 showed more variation.
author	Pinarkara,Emine Kayis,Seyit A Hakki,Erdogan E Sag,Ayla
author_facet	Pinarkara,Emine Kayis,Seyit A Hakki,Erdogan E Sag,Ayla
author_sort	Pinarkara,Emine
title	RAPD analysis of seized marijuana (Cannabis sativa L.) in Turkey
title_short	RAPD analysis of seized marijuana (Cannabis sativa L.) in Turkey
title_full	RAPD analysis of seized marijuana (Cannabis sativa L.) in Turkey
title_fullStr	RAPD analysis of seized marijuana (Cannabis sativa L.) in Turkey
title_full_unstemmed	RAPD analysis of seized marijuana (Cannabis sativa L.) in Turkey
title_sort	rapd analysis of seized marijuana (cannabis sativa l.) in turkey
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2009
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000100005
work_keys_str_mv	AT pinarkaraemine rapdanalysisofseizedmarijuanacannabissativalinturkey AT kayisseyita rapdanalysisofseizedmarijuanacannabissativalinturkey AT hakkierdogane rapdanalysisofseizedmarijuanacannabissativalinturkey AT sagayla rapdanalysisofseizedmarijuanacannabissativalinturkey
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RAPD analysis of seized marijuana (Cannabis sativa L.) in Turkey

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