Influence of metal ions and organic carbons on denitrification activity of the halotolerant bacterium, Paracoccus pantotrophus P16 a strain from shrimp pond

The effect of metal ions, ferric ion (Fe3+) and molybdenum ion (Mo6+) on the denitrification process of Paracoccus pantotrophus P16 grown under saline conditions was investigated. Results revealed that the dosages of added Fe3+ and Mo6+ significantly accelerated nitrate utilization and nitrite accum...

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Autores principales: Pintathong,Punyawatt, Richardson,David J, Spiro,Stephen, Choorit,Wanna
Lenguaje:English
Publicado: Pontificia Universidad Católica de Valparaíso 2009
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Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000200004
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spelling oai:scielo:S0717-345820090002000042009-10-20Influence of metal ions and organic carbons on denitrification activity of the halotolerant bacterium, Paracoccus pantotrophus P16 a strain from shrimp pondPintathong,PunyawattRichardson,David JSpiro,StephenChoorit,Wanna metal ions nitrate reductase nitrite accumulation nitrite reductase Paracoccus pantotrophus The effect of metal ions, ferric ion (Fe3+) and molybdenum ion (Mo6+) on the denitrification process of Paracoccus pantotrophus P16 grown under saline conditions was investigated. Results revealed that the dosages of added Fe3+ and Mo6+ significantly accelerated nitrate utilization and nitrite accumulation. Enzymatic studies revealed that the membrane-bound nitrate reductase and the periplasmic nitrite reductase had activities of 998 ± 28 and 373 ± 18 nmol (mg protein)-1 min-1, respectively after growing Paracoccus pantotrophus P16 in medium supplemented with 1.5 µM Fe3+. If provided with 1.5 µM Fe3+and 2.4 µM Mo6+, the membrane-bound nitrate reductase activity increased to 6,223 ± 502 nmol (mg protein)-1 min-1 and the periplasmic nitrite reductase was 344 ± 20 nmol (mg protein)-1 min-1. The results indicated that an addition of Fe3+ and Mo6+ led to an overstimulation of nitrate reductase activity as compared with nitrite reductase activity. When glucose was supplied, the minimal ratio of carbon per nitrate (C/N) was 2.31 mg C/mg NO3--N with denitrification yield of 0.45 g NO3--N/g C. Addition of ethanol instead of glucose, the minimal ratio of C/N was 1.15 mg C/mg NO3--N with denitrification yield of 1.08 g NO3--N/g C.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.12 n.2 20092009-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000200004en10.4067/S0717-34582009000200004
institution Scielo Chile
collection Scielo Chile
language English
topic metal ions
nitrate reductase
nitrite accumulation
nitrite reductase
Paracoccus pantotrophus
spellingShingle metal ions
nitrate reductase
nitrite accumulation
nitrite reductase
Paracoccus pantotrophus
Pintathong,Punyawatt
Richardson,David J
Spiro,Stephen
Choorit,Wanna
Influence of metal ions and organic carbons on denitrification activity of the halotolerant bacterium, Paracoccus pantotrophus P16 a strain from shrimp pond
description The effect of metal ions, ferric ion (Fe3+) and molybdenum ion (Mo6+) on the denitrification process of Paracoccus pantotrophus P16 grown under saline conditions was investigated. Results revealed that the dosages of added Fe3+ and Mo6+ significantly accelerated nitrate utilization and nitrite accumulation. Enzymatic studies revealed that the membrane-bound nitrate reductase and the periplasmic nitrite reductase had activities of 998 ± 28 and 373 ± 18 nmol (mg protein)-1 min-1, respectively after growing Paracoccus pantotrophus P16 in medium supplemented with 1.5 µM Fe3+. If provided with 1.5 µM Fe3+and 2.4 µM Mo6+, the membrane-bound nitrate reductase activity increased to 6,223 ± 502 nmol (mg protein)-1 min-1 and the periplasmic nitrite reductase was 344 ± 20 nmol (mg protein)-1 min-1. The results indicated that an addition of Fe3+ and Mo6+ led to an overstimulation of nitrate reductase activity as compared with nitrite reductase activity. When glucose was supplied, the minimal ratio of carbon per nitrate (C/N) was 2.31 mg C/mg NO3--N with denitrification yield of 0.45 g NO3--N/g C. Addition of ethanol instead of glucose, the minimal ratio of C/N was 1.15 mg C/mg NO3--N with denitrification yield of 1.08 g NO3--N/g C.
author Pintathong,Punyawatt
Richardson,David J
Spiro,Stephen
Choorit,Wanna
author_facet Pintathong,Punyawatt
Richardson,David J
Spiro,Stephen
Choorit,Wanna
author_sort Pintathong,Punyawatt
title Influence of metal ions and organic carbons on denitrification activity of the halotolerant bacterium, Paracoccus pantotrophus P16 a strain from shrimp pond
title_short Influence of metal ions and organic carbons on denitrification activity of the halotolerant bacterium, Paracoccus pantotrophus P16 a strain from shrimp pond
title_full Influence of metal ions and organic carbons on denitrification activity of the halotolerant bacterium, Paracoccus pantotrophus P16 a strain from shrimp pond
title_fullStr Influence of metal ions and organic carbons on denitrification activity of the halotolerant bacterium, Paracoccus pantotrophus P16 a strain from shrimp pond
title_full_unstemmed Influence of metal ions and organic carbons on denitrification activity of the halotolerant bacterium, Paracoccus pantotrophus P16 a strain from shrimp pond
title_sort influence of metal ions and organic carbons on denitrification activity of the halotolerant bacterium, paracoccus pantotrophus p16 a strain from shrimp pond
publisher Pontificia Universidad Católica de Valparaíso
publishDate 2009
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000200004
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