A rapid and cheap protocol for preparation of PCR templates in peanut

A rapid and cheap protocol for preparation of PCR templates in peanut

This paper describes a simple, low cost and reliable DNA template preparation protocol for polymerase chain reaction (PCR) using immature leaves from peanut seeds or leaves from field-grown plants. The technique may find wide utility in studies involving PCR-based molecular markers, rapid screening...

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Autores principales: Wang,Chuan Tang, Wang,Xiu Zhen, Tang,Yue Yi, Zhang,Jian Cheng, Yu,Shan Lin, Xu,Jian Zhi, Bao,Zhen Min
Lenguaje:English
Publicado: Pontificia Universidad Católica de Valparaíso 2009
Materias:
DNA extraction
groundnut
PCR
peanut
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000200009
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spelling oai:scielo:S0717-345820090002000092009-10-20A rapid and cheap protocol for preparation of PCR templates in peanutWang,Chuan TangWang,Xiu ZhenTang,Yue YiZhang,Jian ChengYu,Shan LinXu,Jian ZhiBao,Zhen Min DNA extraction groundnut PCR peanut This paper describes a simple, low cost and reliable DNA template preparation protocol for polymerase chain reaction (PCR) using immature leaves from peanut seeds or leaves from field-grown plants. The technique may find wide utility in studies involving PCR-based molecular markers, rapid screening for transformants and gene cloning.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.12 n.2 20092009-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000200009en10.4067/S0717-34582009000200009
institution Scielo Chile
collection Scielo Chile
language English
topic DNA extraction
groundnut
PCR
peanut
spellingShingle DNA extraction
groundnut
PCR
peanut
Wang,Chuan Tang
Wang,Xiu Zhen
Tang,Yue Yi
Zhang,Jian Cheng
Yu,Shan Lin
Xu,Jian Zhi
Bao,Zhen Min
A rapid and cheap protocol for preparation of PCR templates in peanut
description This paper describes a simple, low cost and reliable DNA template preparation protocol for polymerase chain reaction (PCR) using immature leaves from peanut seeds or leaves from field-grown plants. The technique may find wide utility in studies involving PCR-based molecular markers, rapid screening for transformants and gene cloning.
author Wang,Chuan Tang
Wang,Xiu Zhen
Tang,Yue Yi
Zhang,Jian Cheng
Yu,Shan Lin
Xu,Jian Zhi
Bao,Zhen Min
author_facet Wang,Chuan Tang
Wang,Xiu Zhen
Tang,Yue Yi
Zhang,Jian Cheng
Yu,Shan Lin
Xu,Jian Zhi
Bao,Zhen Min
author_sort Wang,Chuan Tang
title A rapid and cheap protocol for preparation of PCR templates in peanut
title_short A rapid and cheap protocol for preparation of PCR templates in peanut
title_full A rapid and cheap protocol for preparation of PCR templates in peanut
title_fullStr A rapid and cheap protocol for preparation of PCR templates in peanut
title_full_unstemmed A rapid and cheap protocol for preparation of PCR templates in peanut
title_sort rapid and cheap protocol for preparation of pcr templates in peanut
publisher Pontificia Universidad Católica de Valparaíso
publishDate 2009
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000200009
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