Isolation of high quality DNA: a protocol combining “rennet” and glass milk
High quality DNA is essential for many molecular biology techniques. However, the reagents used for that purpose usually are expensive and/or cause a high environmental impact. Here, we describe two alternative protocols that use inexpensive reagents and are not hazardous to the environment. The fir...
Guardado en:
| Autores principales: | , , |
|---|---|
| Lenguaje: | English |
| Publicado: |
Pontificia Universidad Católica de Valparaíso
2009
|
| Materias: | |
| Acceso en línea: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000200011 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:scielo:S0717-34582009000200011 |
|---|---|
| record_format |
dspace |
| spelling |
oai:scielo:S0717-345820090002000112009-10-20Isolation of high quality DNA: a protocol combining “rennet” and glass milkValter de Oliveira,Luiz FelipeWallau,Gabriel da LuzSilva Loreto,Elgion Lucio chymosin DNA extraction glass milk rennet rennin silica High quality DNA is essential for many molecular biology techniques. However, the reagents used for that purpose usually are expensive and/or cause a high environmental impact. Here, we describe two alternative protocols that use inexpensive reagents and are not hazardous to the environment. The first protocol utilizes the enzyme chymosin, normally used as “rennet” in cheese production and which is easily obtained on the commercial market. The second protocol uses “rennet DNA extraction protocol” combined with the DNA binding capacity of glass powder (glass milk), which can easily be “home made”. The first protocol is used when a high yield of DNA is needed, whereas the second protocol is used for production of a higher quality DNA, being able to work with sparse samples.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.12 n.2 20092009-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000200011en10.4067/S0717-34582009000200011 |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| topic |
chymosin DNA extraction glass milk rennet rennin silica |
| spellingShingle |
chymosin DNA extraction glass milk rennet rennin silica Valter de Oliveira,Luiz Felipe Wallau,Gabriel da Luz Silva Loreto,Elgion Lucio Isolation of high quality DNA: a protocol combining “rennet” and glass milk |
| description |
High quality DNA is essential for many molecular biology techniques. However, the reagents used for that purpose usually are expensive and/or cause a high environmental impact. Here, we describe two alternative protocols that use inexpensive reagents and are not hazardous to the environment. The first protocol utilizes the enzyme chymosin, normally used as “rennet” in cheese production and which is easily obtained on the commercial market. The second protocol uses “rennet DNA extraction protocol” combined with the DNA binding capacity of glass powder (glass milk), which can easily be “home made”. The first protocol is used when a high yield of DNA is needed, whereas the second protocol is used for production of a higher quality DNA, being able to work with sparse samples. |
| author |
Valter de Oliveira,Luiz Felipe Wallau,Gabriel da Luz Silva Loreto,Elgion Lucio |
| author_facet |
Valter de Oliveira,Luiz Felipe Wallau,Gabriel da Luz Silva Loreto,Elgion Lucio |
| author_sort |
Valter de Oliveira,Luiz Felipe |
| title |
Isolation of high quality DNA: a protocol combining “rennet” and glass milk |
| title_short |
Isolation of high quality DNA: a protocol combining “rennet” and glass milk |
| title_full |
Isolation of high quality DNA: a protocol combining “rennet” and glass milk |
| title_fullStr |
Isolation of high quality DNA: a protocol combining “rennet” and glass milk |
| title_full_unstemmed |
Isolation of high quality DNA: a protocol combining “rennet” and glass milk |
| title_sort |
isolation of high quality dna: a protocol combining “rennet” and glass milk |
| publisher |
Pontificia Universidad Católica de Valparaíso |
| publishDate |
2009 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000200011 |
| work_keys_str_mv |
AT valterdeoliveiraluizfelipe isolationofhighqualitydnaaprotocolcombining8220rennet8221andglassmilk AT wallaugabrieldaluz isolationofhighqualitydnaaprotocolcombining8220rennet8221andglassmilk AT silvaloretoelgionlucio isolationofhighqualitydnaaprotocolcombining8220rennet8221andglassmilk |
| _version_ |
1718441798805749760 |