Measuring b-Galactosidase activity at pH 6 with a differential pH sensor

The b-Galactosidase activity at pH 6 is used as a cellular marker to identify senescent cell cultures. The classic method to identify this enzymatic activity is using cytochemical staining with X-Gal after 16 hrs. In this work, a differential pH sensor was used to measure b-Galactosidase activity at...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Acevedo,Cristian, Stach,Matthias H, Amtmann,Anette, Young,Manuel E, Reyes,Juan G, Huebner,Holger, Buchholz,Rainer
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2009
Materias:	b-Galactosidase delta milli pH differential pH
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000200012
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0717-34582009000200012
record_format	dspace
spelling	oai:scielo:S0717-345820090002000122009-10-20Measuring b-Galactosidase activity at pH 6 with a differential pH sensorAcevedo,CristianStach,Matthias HAmtmann,AnetteYoung,Manuel EReyes,Juan GHuebner,HolgerBuchholz,Rainer b-Galactosidase delta milli pH differential pH The b-Galactosidase activity at pH 6 is used as a cellular marker to identify senescent cell cultures. The classic method to identify this enzymatic activity is using cytochemical staining with X-Gal after 16 hrs. In this work, a differential pH sensor was used to measure b-Galactosidase activity at pH 6. The measurement is easy and only takes 3 min.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.12 n.2 20092009-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000200012en10.4067/S0717-34582009000200012
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	b-Galactosidase delta milli pH differential pH
spellingShingle	b-Galactosidase delta milli pH differential pH Acevedo,Cristian Stach,Matthias H Amtmann,Anette Young,Manuel E Reyes,Juan G Huebner,Holger Buchholz,Rainer Measuring b-Galactosidase activity at pH 6 with a differential pH sensor
description	The b-Galactosidase activity at pH 6 is used as a cellular marker to identify senescent cell cultures. The classic method to identify this enzymatic activity is using cytochemical staining with X-Gal after 16 hrs. In this work, a differential pH sensor was used to measure b-Galactosidase activity at pH 6. The measurement is easy and only takes 3 min.
author	Acevedo,Cristian Stach,Matthias H Amtmann,Anette Young,Manuel E Reyes,Juan G Huebner,Holger Buchholz,Rainer
author_facet	Acevedo,Cristian Stach,Matthias H Amtmann,Anette Young,Manuel E Reyes,Juan G Huebner,Holger Buchholz,Rainer
author_sort	Acevedo,Cristian
title	Measuring b-Galactosidase activity at pH 6 with a differential pH sensor
title_short	Measuring b-Galactosidase activity at pH 6 with a differential pH sensor
title_full	Measuring b-Galactosidase activity at pH 6 with a differential pH sensor
title_fullStr	Measuring b-Galactosidase activity at pH 6 with a differential pH sensor
title_full_unstemmed	Measuring b-Galactosidase activity at pH 6 with a differential pH sensor
title_sort	measuring b-galactosidase activity at ph 6 with a differential ph sensor
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2009
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000200012
work_keys_str_mv	AT acevedocristian measuringbgalactosidaseactivityatph6withadifferentialphsensor AT stachmatthiash measuringbgalactosidaseactivityatph6withadifferentialphsensor AT amtmannanette measuringbgalactosidaseactivityatph6withadifferentialphsensor AT youngmanuele measuringbgalactosidaseactivityatph6withadifferentialphsensor AT reyesjuang measuringbgalactosidaseactivityatph6withadifferentialphsensor AT huebnerholger measuringbgalactosidaseactivityatph6withadifferentialphsensor AT buchholzrainer measuringbgalactosidaseactivityatph6withadifferentialphsensor
_version_	1718441799075233792

Measuring b-Galactosidase activity at pH 6 with a differential pH sensor

Ejemplares similares