Diversity of marine gliding bacteria in Thailand and their cytotoxicity

Eighty-four marine gliding bacteria were isolated from specimens collected in the Gulf of Thailand and the Andaman Sea. All exhibited gliding motility and swarm colonies on cultivation plates and they were purified by subculturing and micromanipulator techniques. Their 16S rRNA genes were amplified...

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Autores principales:	Sangnoi,Yutthapong, Srisukchayakul,Pornpoj, Arunpairojana,Vullapa, Kanjana-Opas,Akkharawit
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2009
Materias:	Aureispira marina Aureispira maritime Fulvivirga kasyanovii human cell lines Rapidithrix thailandica Tenacibaculum mesophilum
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000300003
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spelling	oai:scielo:S0717-345820090003000032010-04-01Diversity of marine gliding bacteria in Thailand and their cytotoxicitySangnoi,YutthapongSrisukchayakul,PornpojArunpairojana,VullapaKanjana-Opas,Akkharawit Aureispira marina Aureispira maritime Fulvivirga kasyanovii human cell lines Rapidithrix thailandica Tenacibaculum mesophilum Eighty-four marine gliding bacteria were isolated from specimens collected in the Gulf of Thailand and the Andaman Sea. All exhibited gliding motility and swarm colonies on cultivation plates and they were purified by subculturing and micromanipulator techniques. Their 16S rRNA genes were amplified by the polymerase chain reaction (PCR) technique. The phylogenetic analysis indicated that the represented isolates can be separated into six different clads (gr 1 - gr 6) within the Cytophaga-Flavobacterium-Bacteriodes (CFB) group. Group 1 formed a remote linear, with only 90% sequence similarity, from Flavobacteriaceae bacterium which indicated a potentially novel taxonomic group. Groups 2 and 3 were identified as the recently proposed Tenacibaculum mesophilum and Fulvivirga kasyanovii respectively. Groups 4, 5 and 6, consisting of the largest number of the members, were identified as Rapidithrix thailandica, Aureispira marina and Aureispira maritima respectively. The isolates were cultivated in four different cultivation media (Vy/2, RL 1, CY and SK) and the crude extracts were submitted to screen cytotoxicity using a sulphorodamine B (SRB) assay. The results from cytotoxic screening showed that groups 2, 4 and 6 were capable of producing the cytotoxic metabolites against selected human cell lines (breast adenocarcinoma (MCF-7), colon cancer (HT-29), cervical cancer (HeLa) and oral cancer (KB)). However, groups 1, 3 and 5 did not produce metabolites with cytotoxicity when cultivated in the same cultivation media as the previous groups. CY medium was the only cultivation medium which could yield the cytotoxic metabolites against MCF-7.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.12 n.3 20092009-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000300003en10.4067/S0717-34582009000300003
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Aureispira marina Aureispira maritime Fulvivirga kasyanovii human cell lines Rapidithrix thailandica Tenacibaculum mesophilum
spellingShingle	Aureispira marina Aureispira maritime Fulvivirga kasyanovii human cell lines Rapidithrix thailandica Tenacibaculum mesophilum Sangnoi,Yutthapong Srisukchayakul,Pornpoj Arunpairojana,Vullapa Kanjana-Opas,Akkharawit Diversity of marine gliding bacteria in Thailand and their cytotoxicity
description	Eighty-four marine gliding bacteria were isolated from specimens collected in the Gulf of Thailand and the Andaman Sea. All exhibited gliding motility and swarm colonies on cultivation plates and they were purified by subculturing and micromanipulator techniques. Their 16S rRNA genes were amplified by the polymerase chain reaction (PCR) technique. The phylogenetic analysis indicated that the represented isolates can be separated into six different clads (gr 1 - gr 6) within the Cytophaga-Flavobacterium-Bacteriodes (CFB) group. Group 1 formed a remote linear, with only 90% sequence similarity, from Flavobacteriaceae bacterium which indicated a potentially novel taxonomic group. Groups 2 and 3 were identified as the recently proposed Tenacibaculum mesophilum and Fulvivirga kasyanovii respectively. Groups 4, 5 and 6, consisting of the largest number of the members, were identified as Rapidithrix thailandica, Aureispira marina and Aureispira maritima respectively. The isolates were cultivated in four different cultivation media (Vy/2, RL 1, CY and SK) and the crude extracts were submitted to screen cytotoxicity using a sulphorodamine B (SRB) assay. The results from cytotoxic screening showed that groups 2, 4 and 6 were capable of producing the cytotoxic metabolites against selected human cell lines (breast adenocarcinoma (MCF-7), colon cancer (HT-29), cervical cancer (HeLa) and oral cancer (KB)). However, groups 1, 3 and 5 did not produce metabolites with cytotoxicity when cultivated in the same cultivation media as the previous groups. CY medium was the only cultivation medium which could yield the cytotoxic metabolites against MCF-7.
author	Sangnoi,Yutthapong Srisukchayakul,Pornpoj Arunpairojana,Vullapa Kanjana-Opas,Akkharawit
author_facet	Sangnoi,Yutthapong Srisukchayakul,Pornpoj Arunpairojana,Vullapa Kanjana-Opas,Akkharawit
author_sort	Sangnoi,Yutthapong
title	Diversity of marine gliding bacteria in Thailand and their cytotoxicity
title_short	Diversity of marine gliding bacteria in Thailand and their cytotoxicity
title_full	Diversity of marine gliding bacteria in Thailand and their cytotoxicity
title_fullStr	Diversity of marine gliding bacteria in Thailand and their cytotoxicity
title_full_unstemmed	Diversity of marine gliding bacteria in Thailand and their cytotoxicity
title_sort	diversity of marine gliding bacteria in thailand and their cytotoxicity
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2009
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000300003
work_keys_str_mv	AT sangnoiyutthapong diversityofmarineglidingbacteriainthailandandtheircytotoxicity AT srisukchayakulpornpoj diversityofmarineglidingbacteriainthailandandtheircytotoxicity AT arunpairojanavullapa diversityofmarineglidingbacteriainthailandandtheircytotoxicity AT kanjanaopasakkharawit diversityofmarineglidingbacteriainthailandandtheircytotoxicity
_version_	1718441800164704256

Diversity of marine gliding bacteria in Thailand and their cytotoxicity

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