Establishment of an efficient callus induction and plant regeneration system in Pakistani wheat (Triticum aestivum) cultivars

Four commercially grown wheat varieties of Pakistan, namely Inqilab-91, Chakwal-97, Tatara and Manthar were used for this investigation. For callus induction different concentrations of 2,4-Dichlorophenoxyaceticacid (2,4-D) along with 0.1 mg/L of Kinetin were evaluated. For regeneration initially di...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Rashid,Umer, Ali,Shaukat, Ali,Ghulam Muhammad, Ayub,Najma, Masood,M. Shahid
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2009
Materias:	2,4-D 2iP BAP callus induction IAA kinetin regeneration wheat
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000300004
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0717-34582009000300004
record_format	dspace
spelling	oai:scielo:S0717-345820090003000042010-04-01Establishment of an efficient callus induction and plant regeneration system in Pakistani wheat (Triticum aestivum) cultivarsRashid,UmerAli,ShaukatAli,Ghulam MuhammadAyub,NajmaMasood,M. Shahid 2,4-D 2iP BAP callus induction IAA kinetin regeneration wheat Four commercially grown wheat varieties of Pakistan, namely Inqilab-91, Chakwal-97, Tatara and Manthar were used for this investigation. For callus induction different concentrations of 2,4-Dichlorophenoxyaceticacid (2,4-D) along with 0.1 mg/L of Kinetin were evaluated. For regeneration initially different concentrations of Indole-3-Acetic Acid (IAA) and 6-BenzylAminoPurine (BAP) were tested. Best hormone combinations were further subjected to Kinetin and 6-γ-γ-dimethylallylaminopurine (2iP). For Inqilab-91, Chakwal-97 and Manthar, 3 mg/L of 2,4-D was found optimum, which induced 83.25%, 77.75% and 95.20% of embryogenic calli, respectively. Maximum callus induction (97.18%) was observed in Tatara when 2 mg/L of 2,4-D was used. As regard to regeneration, Inqilab-91, Chakwal-97 and Manthar showed maximum regeneration on media containing 0.1 mg/L IAA, 0.4 mg/L Kinetin and 0.5 mg/L 2iP, regenerating 87.25%, 81.75% and 68.75% respectively. For Tatara maximum regeneration of 12.25% was obtained on 0.1 mg/L IAA and 2 mg/L of BAP. Presently optimized regeneration method holds promise for facilitating the deployment of agronomical important trait through genetic transformation for the improvement of this important food crop.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.12 n.3 20092009-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000300004en10.4067/S0717-34582009000300004
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	2,4-D 2iP BAP callus induction IAA kinetin regeneration wheat
spellingShingle	2,4-D 2iP BAP callus induction IAA kinetin regeneration wheat Rashid,Umer Ali,Shaukat Ali,Ghulam Muhammad Ayub,Najma Masood,M. Shahid Establishment of an efficient callus induction and plant regeneration system in Pakistani wheat (Triticum aestivum) cultivars
description	Four commercially grown wheat varieties of Pakistan, namely Inqilab-91, Chakwal-97, Tatara and Manthar were used for this investigation. For callus induction different concentrations of 2,4-Dichlorophenoxyaceticacid (2,4-D) along with 0.1 mg/L of Kinetin were evaluated. For regeneration initially different concentrations of Indole-3-Acetic Acid (IAA) and 6-BenzylAminoPurine (BAP) were tested. Best hormone combinations were further subjected to Kinetin and 6-γ-γ-dimethylallylaminopurine (2iP). For Inqilab-91, Chakwal-97 and Manthar, 3 mg/L of 2,4-D was found optimum, which induced 83.25%, 77.75% and 95.20% of embryogenic calli, respectively. Maximum callus induction (97.18%) was observed in Tatara when 2 mg/L of 2,4-D was used. As regard to regeneration, Inqilab-91, Chakwal-97 and Manthar showed maximum regeneration on media containing 0.1 mg/L IAA, 0.4 mg/L Kinetin and 0.5 mg/L 2iP, regenerating 87.25%, 81.75% and 68.75% respectively. For Tatara maximum regeneration of 12.25% was obtained on 0.1 mg/L IAA and 2 mg/L of BAP. Presently optimized regeneration method holds promise for facilitating the deployment of agronomical important trait through genetic transformation for the improvement of this important food crop.
author	Rashid,Umer Ali,Shaukat Ali,Ghulam Muhammad Ayub,Najma Masood,M. Shahid
author_facet	Rashid,Umer Ali,Shaukat Ali,Ghulam Muhammad Ayub,Najma Masood,M. Shahid
author_sort	Rashid,Umer
title	Establishment of an efficient callus induction and plant regeneration system in Pakistani wheat (Triticum aestivum) cultivars
title_short	Establishment of an efficient callus induction and plant regeneration system in Pakistani wheat (Triticum aestivum) cultivars
title_full	Establishment of an efficient callus induction and plant regeneration system in Pakistani wheat (Triticum aestivum) cultivars
title_fullStr	Establishment of an efficient callus induction and plant regeneration system in Pakistani wheat (Triticum aestivum) cultivars
title_full_unstemmed	Establishment of an efficient callus induction and plant regeneration system in Pakistani wheat (Triticum aestivum) cultivars
title_sort	establishment of an efficient callus induction and plant regeneration system in pakistani wheat (triticum aestivum) cultivars
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2009
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000300004
work_keys_str_mv	AT rashidumer establishmentofanefficientcallusinductionandplantregenerationsysteminpakistaniwheattriticumaestivumcultivars AT alishaukat establishmentofanefficientcallusinductionandplantregenerationsysteminpakistaniwheattriticumaestivumcultivars AT alighulammuhammad establishmentofanefficientcallusinductionandplantregenerationsysteminpakistaniwheattriticumaestivumcultivars AT ayubnajma establishmentofanefficientcallusinductionandplantregenerationsysteminpakistaniwheattriticumaestivumcultivars AT masoodmshahid establishmentofanefficientcallusinductionandplantregenerationsysteminpakistaniwheattriticumaestivumcultivars
_version_	1718441800449916928

Establishment of an efficient callus induction and plant regeneration system in Pakistani wheat (Triticum aestivum) cultivars

Ejemplares similares