Gene expression and characterization of 2-keto-3-deoxy-gluconate kinase, a key enzyme in the modified Entner-Doudoroff pathway of Serratia marcescens KCTC 2172
We cloned 2-keto-3-deoxy-gluconate kinase (KDGK), which catalyzes the phosphorylation of 2-keto-3-deoxygluconate (KDG) to 2-keto-3-deoxy-6-phophogluconate (KDPG) from Serratia marcescens KCTC 2172. The nucleotide sequence revealed a single open reading frame containing 1,208 bp and encoding for 309...
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Pontificia Universidad Católica de Valparaíso
2009
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oai:scielo:S0717-345820090003000052010-04-01Gene expression and characterization of 2-keto-3-deoxy-gluconate kinase, a key enzyme in the modified Entner-Doudoroff pathway of Serratia marcescens KCTC 2172Lee,Yong-SeokPark,In-HyeYoo,Ju-SoonKim,Hae-SunChung,Soo-YeolChandra,Muni Ramanna GariSubhoshChoi,Yong-Lark 2-keto-3-deoxygluconate kinase carbohydrate kinase purification Serratia marcescens KCTC 2172 We cloned 2-keto-3-deoxy-gluconate kinase (KDGK), which catalyzes the phosphorylation of 2-keto-3-deoxygluconate (KDG) to 2-keto-3-deoxy-6-phophogluconate (KDPG) from Serratia marcescens KCTC 2172. The nucleotide sequence revealed a single open reading frame containing 1,208 bp and encoding for 309 amino acids, with a molecular weight of 33,993 Da. The enzyme was purified via GST affinity chromatography. The putative KdgT binding site was detected upstream of the initial codon. The KDG kinase utilized 2-ketogluconate (KG) and KDG as substrates. The optimal temperature and pH for KDGK activity were 50ºC and 8.0, respectively.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.12 n.3 20092009-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000300005en10.4067/S0717-34582009000300005 |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| topic |
2-keto-3-deoxygluconate kinase carbohydrate kinase purification Serratia marcescens KCTC 2172 |
| spellingShingle |
2-keto-3-deoxygluconate kinase carbohydrate kinase purification Serratia marcescens KCTC 2172 Lee,Yong-Seok Park,In-Hye Yoo,Ju-Soon Kim,Hae-Sun Chung,Soo-Yeol Chandra,Muni Ramanna GariSubhosh Choi,Yong-Lark Gene expression and characterization of 2-keto-3-deoxy-gluconate kinase, a key enzyme in the modified Entner-Doudoroff pathway of Serratia marcescens KCTC 2172 |
| description |
We cloned 2-keto-3-deoxy-gluconate kinase (KDGK), which catalyzes the phosphorylation of 2-keto-3-deoxygluconate (KDG) to 2-keto-3-deoxy-6-phophogluconate (KDPG) from Serratia marcescens KCTC 2172. The nucleotide sequence revealed a single open reading frame containing 1,208 bp and encoding for 309 amino acids, with a molecular weight of 33,993 Da. The enzyme was purified via GST affinity chromatography. The putative KdgT binding site was detected upstream of the initial codon. The KDG kinase utilized 2-ketogluconate (KG) and KDG as substrates. The optimal temperature and pH for KDGK activity were 50ºC and 8.0, respectively. |
| author |
Lee,Yong-Seok Park,In-Hye Yoo,Ju-Soon Kim,Hae-Sun Chung,Soo-Yeol Chandra,Muni Ramanna GariSubhosh Choi,Yong-Lark |
| author_facet |
Lee,Yong-Seok Park,In-Hye Yoo,Ju-Soon Kim,Hae-Sun Chung,Soo-Yeol Chandra,Muni Ramanna GariSubhosh Choi,Yong-Lark |
| author_sort |
Lee,Yong-Seok |
| title |
Gene expression and characterization of 2-keto-3-deoxy-gluconate kinase, a key enzyme in the modified Entner-Doudoroff pathway of Serratia marcescens KCTC 2172 |
| title_short |
Gene expression and characterization of 2-keto-3-deoxy-gluconate kinase, a key enzyme in the modified Entner-Doudoroff pathway of Serratia marcescens KCTC 2172 |
| title_full |
Gene expression and characterization of 2-keto-3-deoxy-gluconate kinase, a key enzyme in the modified Entner-Doudoroff pathway of Serratia marcescens KCTC 2172 |
| title_fullStr |
Gene expression and characterization of 2-keto-3-deoxy-gluconate kinase, a key enzyme in the modified Entner-Doudoroff pathway of Serratia marcescens KCTC 2172 |
| title_full_unstemmed |
Gene expression and characterization of 2-keto-3-deoxy-gluconate kinase, a key enzyme in the modified Entner-Doudoroff pathway of Serratia marcescens KCTC 2172 |
| title_sort |
gene expression and characterization of 2-keto-3-deoxy-gluconate kinase, a key enzyme in the modified entner-doudoroff pathway of serratia marcescens kctc 2172 |
| publisher |
Pontificia Universidad Católica de Valparaíso |
| publishDate |
2009 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000300005 |
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