Identification of alternate dwarfing gene sources to widely used Dee-Gee-Woo-Gen allele of sd1 gene by molecular and biochemical assays in rice (Oryza sativa L.)

After the success of IR8 and TN1, breeders depended heavily on these two rice cultivars for source of short stature led to the narrow genetic base to majority of present day rice varieties, as far as sd1 (semi-dwarf1) gene is concerned. In addition, analysis of genetic lineage of the majority of the...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Neeraja,Chirravuri Naga, Vemireddy,Lakshminarayana Reddy, Malathi,Surapaneni, Siddiq,Ebrahimali Abubacker
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2009
Materias:	Dee-Gee-Woo-Gen dwarfing gene gibberellic acid assay molecular marker rice sd1 gene
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000300007
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0717-34582009000300007
record_format	dspace
spelling	oai:scielo:S0717-345820090003000072010-04-01Identification of alternate dwarfing gene sources to widely used Dee-Gee-Woo-Gen allele of sd1 gene by molecular and biochemical assays in rice (Oryza sativa L.)Neeraja,Chirravuri NagaVemireddy,Lakshminarayana ReddyMalathi,SurapaneniSiddiq,Ebrahimali Abubacker Dee-Gee-Woo-Gen dwarfing gene gibberellic acid assay molecular marker rice sd1 gene After the success of IR8 and TN1, breeders depended heavily on these two rice cultivars for source of short stature led to the narrow genetic base to majority of present day rice varieties, as far as sd1 (semi-dwarf1) gene is concerned. In addition, analysis of genetic lineage of the majority of the cultivated rice varieties in tropical Asia reveals that sd1 from DGWG (Dee-Gee-Woo-Gen) is the major source of dwarfing gene. Such high amount of genetic homogeneity renders rice plants vulnerable to epidemic of diseases and insect pests. In the current study, we made an attempt to identify the alternate sources of DGWG allele of sd1 gene by characterizing 29 induced and 3 spontaneous dwarf accessions employing marker for DGWG allele of sd1 gene and exogenous application of gibberellic acid (GA3). When occurrence of DGWG allele of sd1 gene and GA3 response were analyzed together, existence of two kinds of dwarfs was noticed viz., dwarf accessions with DGWG allele and dwarf accessions without DGWG allele of sd1 allele exhibiting varying responses to GA3. As many as 22 of 32 dwarf accessions showed absence of DGWG allele of sd1 gene with varying response to GA3 could be used as excellent alternate sources for DGWG allele of sd1 gene. These dwarf accessions could be used for broadening the genetic base for the plant height and thereby minimize the risk of genetic vulnerability. Our strategy of combining molecular and biochemical assays can be efficiently used for identifying alternate dwarfing gene sources to the Green Revolution gene sd1.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.12 n.3 20092009-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000300007en10.4067/S0717-34582009000300007
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Dee-Gee-Woo-Gen dwarfing gene gibberellic acid assay molecular marker rice sd1 gene
spellingShingle	Dee-Gee-Woo-Gen dwarfing gene gibberellic acid assay molecular marker rice sd1 gene Neeraja,Chirravuri Naga Vemireddy,Lakshminarayana Reddy Malathi,Surapaneni Siddiq,Ebrahimali Abubacker Identification of alternate dwarfing gene sources to widely used Dee-Gee-Woo-Gen allele of sd1 gene by molecular and biochemical assays in rice (Oryza sativa L.)
description	After the success of IR8 and TN1, breeders depended heavily on these two rice cultivars for source of short stature led to the narrow genetic base to majority of present day rice varieties, as far as sd1 (semi-dwarf1) gene is concerned. In addition, analysis of genetic lineage of the majority of the cultivated rice varieties in tropical Asia reveals that sd1 from DGWG (Dee-Gee-Woo-Gen) is the major source of dwarfing gene. Such high amount of genetic homogeneity renders rice plants vulnerable to epidemic of diseases and insect pests. In the current study, we made an attempt to identify the alternate sources of DGWG allele of sd1 gene by characterizing 29 induced and 3 spontaneous dwarf accessions employing marker for DGWG allele of sd1 gene and exogenous application of gibberellic acid (GA3). When occurrence of DGWG allele of sd1 gene and GA3 response were analyzed together, existence of two kinds of dwarfs was noticed viz., dwarf accessions with DGWG allele and dwarf accessions without DGWG allele of sd1 allele exhibiting varying responses to GA3. As many as 22 of 32 dwarf accessions showed absence of DGWG allele of sd1 gene with varying response to GA3 could be used as excellent alternate sources for DGWG allele of sd1 gene. These dwarf accessions could be used for broadening the genetic base for the plant height and thereby minimize the risk of genetic vulnerability. Our strategy of combining molecular and biochemical assays can be efficiently used for identifying alternate dwarfing gene sources to the Green Revolution gene sd1.
author	Neeraja,Chirravuri Naga Vemireddy,Lakshminarayana Reddy Malathi,Surapaneni Siddiq,Ebrahimali Abubacker
author_facet	Neeraja,Chirravuri Naga Vemireddy,Lakshminarayana Reddy Malathi,Surapaneni Siddiq,Ebrahimali Abubacker
author_sort	Neeraja,Chirravuri Naga
title	Identification of alternate dwarfing gene sources to widely used Dee-Gee-Woo-Gen allele of sd1 gene by molecular and biochemical assays in rice (Oryza sativa L.)
title_short	Identification of alternate dwarfing gene sources to widely used Dee-Gee-Woo-Gen allele of sd1 gene by molecular and biochemical assays in rice (Oryza sativa L.)
title_full	Identification of alternate dwarfing gene sources to widely used Dee-Gee-Woo-Gen allele of sd1 gene by molecular and biochemical assays in rice (Oryza sativa L.)
title_fullStr	Identification of alternate dwarfing gene sources to widely used Dee-Gee-Woo-Gen allele of sd1 gene by molecular and biochemical assays in rice (Oryza sativa L.)
title_full_unstemmed	Identification of alternate dwarfing gene sources to widely used Dee-Gee-Woo-Gen allele of sd1 gene by molecular and biochemical assays in rice (Oryza sativa L.)
title_sort	identification of alternate dwarfing gene sources to widely used dee-gee-woo-gen allele of sd1 gene by molecular and biochemical assays in rice (oryza sativa l.)
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2009
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000300007
work_keys_str_mv	AT neerajachirravurinaga identificationofalternatedwarfinggenesourcestowidelyuseddeegeewoogenalleleofsd1genebymolecularandbiochemicalassaysinriceoryzasatival AT vemireddylakshminarayanareddy identificationofalternatedwarfinggenesourcestowidelyuseddeegeewoogenalleleofsd1genebymolecularandbiochemicalassaysinriceoryzasatival AT malathisurapaneni identificationofalternatedwarfinggenesourcestowidelyuseddeegeewoogenalleleofsd1genebymolecularandbiochemicalassaysinriceoryzasatival AT siddiqebrahimaliabubacker identificationofalternatedwarfinggenesourcestowidelyuseddeegeewoogenalleleofsd1genebymolecularandbiochemicalassaysinriceoryzasatival
_version_	1718441801170288640

Identification of alternate dwarfing gene sources to widely used Dee-Gee-Woo-Gen allele of sd1 gene by molecular and biochemical assays in rice (Oryza sativa L.)

Ejemplares similares