Influence of buffer systems on Trichoderma reesei Rut C-30 morphology and cellulase production

The cellulase enzyme production is a key issue in the enzymatic hydrolysis of lignocellulosic materials. Since fungal morphology influences the productivity of fungal fermentations, it is of major importance to well know the fungal behavior during culture for cellulase production. In this work, the...

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Autores principales:	Ferreira,Susana M. P., Duarte,Ana P., Queiroz,João A., Domingues,Fernanda C.
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2009
Materias:	buffer systems cellulase fungal morphology submerged cell growth Trichoderma reesei Rut C-30
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000300008
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spelling	oai:scielo:S0717-345820090003000082010-04-01Influence of buffer systems on Trichoderma reesei Rut C-30 morphology and cellulase productionFerreira,Susana M. P.Duarte,Ana P.Queiroz,João A.Domingues,Fernanda C. buffer systems cellulase fungal morphology submerged cell growth Trichoderma reesei Rut C-30 The cellulase enzyme production is a key issue in the enzymatic hydrolysis of lignocellulosic materials. Since fungal morphology influences the productivity of fungal fermentations, it is of major importance to well know the fungal behavior during culture for cellulase production. In this work, the influence of medium supplementation, with different buffer systems at two different concentrations and pH conditions, on the morphology of T. reesei Rut C-30 and cellulase production, was investigated. A medium without buffer was used as control. The results suggest that fungal morphology is significantly dependent on the addition of different buffer systems to the nutrient broth. The mycelial morphology shows a clear transition from clumped to pelleted forms in cultures with variation of buffer systems and concentration. The higher filter paper activity was obtained using 100 mM succinate buffer, at pH 4.8, in the medium supplementation, corresponding to a dispersed mycelial morphology.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.12 n.3 20092009-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000300008en10.4067/S0717-34582009000300008
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	buffer systems cellulase fungal morphology submerged cell growth Trichoderma reesei Rut C-30
spellingShingle	buffer systems cellulase fungal morphology submerged cell growth Trichoderma reesei Rut C-30 Ferreira,Susana M. P. Duarte,Ana P. Queiroz,João A. Domingues,Fernanda C. Influence of buffer systems on Trichoderma reesei Rut C-30 morphology and cellulase production
description	The cellulase enzyme production is a key issue in the enzymatic hydrolysis of lignocellulosic materials. Since fungal morphology influences the productivity of fungal fermentations, it is of major importance to well know the fungal behavior during culture for cellulase production. In this work, the influence of medium supplementation, with different buffer systems at two different concentrations and pH conditions, on the morphology of T. reesei Rut C-30 and cellulase production, was investigated. A medium without buffer was used as control. The results suggest that fungal morphology is significantly dependent on the addition of different buffer systems to the nutrient broth. The mycelial morphology shows a clear transition from clumped to pelleted forms in cultures with variation of buffer systems and concentration. The higher filter paper activity was obtained using 100 mM succinate buffer, at pH 4.8, in the medium supplementation, corresponding to a dispersed mycelial morphology.
author	Ferreira,Susana M. P. Duarte,Ana P. Queiroz,João A. Domingues,Fernanda C.
author_facet	Ferreira,Susana M. P. Duarte,Ana P. Queiroz,João A. Domingues,Fernanda C.
author_sort	Ferreira,Susana M. P.
title	Influence of buffer systems on Trichoderma reesei Rut C-30 morphology and cellulase production
title_short	Influence of buffer systems on Trichoderma reesei Rut C-30 morphology and cellulase production
title_full	Influence of buffer systems on Trichoderma reesei Rut C-30 morphology and cellulase production
title_fullStr	Influence of buffer systems on Trichoderma reesei Rut C-30 morphology and cellulase production
title_full_unstemmed	Influence of buffer systems on Trichoderma reesei Rut C-30 morphology and cellulase production
title_sort	influence of buffer systems on trichoderma reesei rut c-30 morphology and cellulase production
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2009
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000300008
work_keys_str_mv	AT ferreirasusanamp influenceofbuffersystemsontrichodermareeseirutc30morphologyandcellulaseproduction AT duarteanap influenceofbuffersystemsontrichodermareeseirutc30morphologyandcellulaseproduction AT queirozjoaoa influenceofbuffersystemsontrichodermareeseirutc30morphologyandcellulaseproduction AT dominguesfernandac influenceofbuffersystemsontrichodermareeseirutc30morphologyandcellulaseproduction
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Influence of buffer systems on Trichoderma reesei Rut C-30 morphology and cellulase production

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