Micro-scale surface-patterning influences biofilm formation

The formation of biofilms on indwelling/implanted medical devices is a common problem. One of the approaches used to prevent biofilm formation on medical devices is to inhibit bacterial attachment by modification of the synthetic polymers used to fabricate the device. In this work, we assessed how m...

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Autores principales:	Kappell,Guimel M, Grover,James P, Chrzanowski,Thomas H
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2009
Materias:	indwelling medical devices infections surface attachment
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000300010
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spelling	oai:scielo:S0717-345820090003000102010-04-01Micro-scale surface-patterning influences biofilm formationKappell,Guimel MGrover,James PChrzanowski,Thomas H indwelling medical devices infections surface attachment The formation of biofilms on indwelling/implanted medical devices is a common problem. One of the approaches used to prevent biofilm formation on medical devices is to inhibit bacterial attachment by modification of the synthetic polymers used to fabricate the device. In this work, we assessed how micro-scale features (patterns) imprinted onto the surface of silicone elastomer similar to that used for medical applications influenced biofilm formation by Staphylococcus aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa. Patterns were transferred from a multi-patterned oxidized silicon-wafer master-template to silicone elastomer. Features consisted of bars, squares, and circles each extending 0.51 µm above the surface. Feature sizes ranged between 1.78 and 22.25 µm. Distances separating features ranged between 0.26 and 17.35 µm. Bacterial biofilm formation on discs cut from imprinted silicone elastomer was assessed by direct microscopic observation and quantified as the surface area covered by biofilm. Unpatterned silicone elastomer served as a control. Several of the micro-scale patterns imprinted into the silicone elastomer significantly reduced biofilm formation by each bacterium and interrupted biofilm continuity. Although there were differences in detail among strains, bacteria tended to attach in the area between features more than to the surface of the feature itself.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.12 n.3 20092009-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000300010en10.4067/S0717-34582009000300010
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	indwelling medical devices infections surface attachment
spellingShingle	indwelling medical devices infections surface attachment Kappell,Guimel M Grover,James P Chrzanowski,Thomas H Micro-scale surface-patterning influences biofilm formation
description	The formation of biofilms on indwelling/implanted medical devices is a common problem. One of the approaches used to prevent biofilm formation on medical devices is to inhibit bacterial attachment by modification of the synthetic polymers used to fabricate the device. In this work, we assessed how micro-scale features (patterns) imprinted onto the surface of silicone elastomer similar to that used for medical applications influenced biofilm formation by Staphylococcus aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa. Patterns were transferred from a multi-patterned oxidized silicon-wafer master-template to silicone elastomer. Features consisted of bars, squares, and circles each extending 0.51 µm above the surface. Feature sizes ranged between 1.78 and 22.25 µm. Distances separating features ranged between 0.26 and 17.35 µm. Bacterial biofilm formation on discs cut from imprinted silicone elastomer was assessed by direct microscopic observation and quantified as the surface area covered by biofilm. Unpatterned silicone elastomer served as a control. Several of the micro-scale patterns imprinted into the silicone elastomer significantly reduced biofilm formation by each bacterium and interrupted biofilm continuity. Although there were differences in detail among strains, bacteria tended to attach in the area between features more than to the surface of the feature itself.
author	Kappell,Guimel M Grover,James P Chrzanowski,Thomas H
author_facet	Kappell,Guimel M Grover,James P Chrzanowski,Thomas H
author_sort	Kappell,Guimel M
title	Micro-scale surface-patterning influences biofilm formation
title_short	Micro-scale surface-patterning influences biofilm formation
title_full	Micro-scale surface-patterning influences biofilm formation
title_fullStr	Micro-scale surface-patterning influences biofilm formation
title_full_unstemmed	Micro-scale surface-patterning influences biofilm formation
title_sort	micro-scale surface-patterning influences biofilm formation
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2009
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000300010
work_keys_str_mv	AT kappellguimelm microscalesurfacepatterninginfluencesbiofilmformation AT groverjamesp microscalesurfacepatterninginfluencesbiofilmformation AT chrzanowskithomash microscalesurfacepatterninginfluencesbiofilmformation
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Micro-scale surface-patterning influences biofilm formation

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