PCR-based DGGE and FISH analysis of methanogens in an anaerobic closed digester tank for treating palm oil mill effluent

16S ribosomal RNA (rRNA)-targeted fluorescent in situ hybridization combined with polymerase chain reaction (PCR)-cloning, light microscopy using Gram stains, scanning electron microscopy and denatured gradient gel electrophoresis were used to reveal the distribution of methanogens within an anaerob...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Tabatabaei,Meisam, Zakaria,Mohd Rafein, Rahim,Raha Abdul, Wright,André-Denis G., Shirai,Yoshihito, Abdullah,Norhani, Sakai,Kenji, Ikeno,Shinya, Mori,Masatsugu, Kazunori,Nakamura, Sulaiman,Alawi, Hassan,Mohd Ali
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2009
Materias:	anaerobic digestion DGGE FISH methanogens POME
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000300012
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0717-34582009000300012
record_format	dspace
spelling	oai:scielo:S0717-345820090003000122010-04-01PCR-based DGGE and FISH analysis of methanogens in an anaerobic closed digester tank for treating palm oil mill effluentTabatabaei,MeisamZakaria,Mohd RafeinRahim,Raha AbdulWright,André-Denis G.Shirai,YoshihitoAbdullah,NorhaniSakai,KenjiIkeno,ShinyaMori,MasatsuguKazunori,NakamuraSulaiman,AlawiHassan,Mohd Ali anaerobic digestion DGGE FISH methanogens POME 16S ribosomal RNA (rRNA)-targeted fluorescent in situ hybridization combined with polymerase chain reaction (PCR)-cloning, light microscopy using Gram stains, scanning electron microscopy and denatured gradient gel electrophoresis were used to reveal the distribution of methanogens within an anaerobic closed digester tank fed with palm oil mill effluent. For specific detection of methanogens, 16S rRNA-cloning analysis was conducted followed by restriction fragment length polymorphism (RFLP) for presumptive identification of methanogens. To cover the drawbacks of the PCR-cloning study, the organization of the microorganisms was visualized in the activated sludge sample by using fluorescent oligonucleotide probes specific to several different methanogens, and a probe for bacteria. In situ hybridization with methanogens and bacterial probes and denatured gradient gel electrophoresis within activated sludge clearly confirmed the presence of Methanosaeta sp. and Methanosarcina sp. cells. Methanosaeta concilii was found to be the dominant species in the bioreactor. These results revealed the presence of possibly new strain of Methanosaeta in the bioreactor for treating palm oil mill effluent called Methanosaeta concilii SamaliEB (Gene bank accession number: EU580025). In addition, fluorescent hybridization pictured the close association between the methanogens and bacteria and that the number of methanogens was greater than the number of bacteria.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.12 n.3 20092009-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000300012en10.4067/S0717-34582009000300012
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	anaerobic digestion DGGE FISH methanogens POME
spellingShingle	anaerobic digestion DGGE FISH methanogens POME Tabatabaei,Meisam Zakaria,Mohd Rafein Rahim,Raha Abdul Wright,André-Denis G. Shirai,Yoshihito Abdullah,Norhani Sakai,Kenji Ikeno,Shinya Mori,Masatsugu Kazunori,Nakamura Sulaiman,Alawi Hassan,Mohd Ali PCR-based DGGE and FISH analysis of methanogens in an anaerobic closed digester tank for treating palm oil mill effluent
description	16S ribosomal RNA (rRNA)-targeted fluorescent in situ hybridization combined with polymerase chain reaction (PCR)-cloning, light microscopy using Gram stains, scanning electron microscopy and denatured gradient gel electrophoresis were used to reveal the distribution of methanogens within an anaerobic closed digester tank fed with palm oil mill effluent. For specific detection of methanogens, 16S rRNA-cloning analysis was conducted followed by restriction fragment length polymorphism (RFLP) for presumptive identification of methanogens. To cover the drawbacks of the PCR-cloning study, the organization of the microorganisms was visualized in the activated sludge sample by using fluorescent oligonucleotide probes specific to several different methanogens, and a probe for bacteria. In situ hybridization with methanogens and bacterial probes and denatured gradient gel electrophoresis within activated sludge clearly confirmed the presence of Methanosaeta sp. and Methanosarcina sp. cells. Methanosaeta concilii was found to be the dominant species in the bioreactor. These results revealed the presence of possibly new strain of Methanosaeta in the bioreactor for treating palm oil mill effluent called Methanosaeta concilii SamaliEB (Gene bank accession number: EU580025). In addition, fluorescent hybridization pictured the close association between the methanogens and bacteria and that the number of methanogens was greater than the number of bacteria.
author	Tabatabaei,Meisam Zakaria,Mohd Rafein Rahim,Raha Abdul Wright,André-Denis G. Shirai,Yoshihito Abdullah,Norhani Sakai,Kenji Ikeno,Shinya Mori,Masatsugu Kazunori,Nakamura Sulaiman,Alawi Hassan,Mohd Ali
author_facet	Tabatabaei,Meisam Zakaria,Mohd Rafein Rahim,Raha Abdul Wright,André-Denis G. Shirai,Yoshihito Abdullah,Norhani Sakai,Kenji Ikeno,Shinya Mori,Masatsugu Kazunori,Nakamura Sulaiman,Alawi Hassan,Mohd Ali
author_sort	Tabatabaei,Meisam
title	PCR-based DGGE and FISH analysis of methanogens in an anaerobic closed digester tank for treating palm oil mill effluent
title_short	PCR-based DGGE and FISH analysis of methanogens in an anaerobic closed digester tank for treating palm oil mill effluent
title_full	PCR-based DGGE and FISH analysis of methanogens in an anaerobic closed digester tank for treating palm oil mill effluent
title_fullStr	PCR-based DGGE and FISH analysis of methanogens in an anaerobic closed digester tank for treating palm oil mill effluent
title_full_unstemmed	PCR-based DGGE and FISH analysis of methanogens in an anaerobic closed digester tank for treating palm oil mill effluent
title_sort	pcr-based dgge and fish analysis of methanogens in an anaerobic closed digester tank for treating palm oil mill effluent
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2009
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000300012
work_keys_str_mv	AT tabatabaeimeisam pcrbaseddggeandfishanalysisofmethanogensinananaerobiccloseddigestertankfortreatingpalmoilmilleffluent AT zakariamohdrafein pcrbaseddggeandfishanalysisofmethanogensinananaerobiccloseddigestertankfortreatingpalmoilmilleffluent AT rahimrahaabdul pcrbaseddggeandfishanalysisofmethanogensinananaerobiccloseddigestertankfortreatingpalmoilmilleffluent AT wrightandredenisg pcrbaseddggeandfishanalysisofmethanogensinananaerobiccloseddigestertankfortreatingpalmoilmilleffluent AT shiraiyoshihito pcrbaseddggeandfishanalysisofmethanogensinananaerobiccloseddigestertankfortreatingpalmoilmilleffluent AT abdullahnorhani pcrbaseddggeandfishanalysisofmethanogensinananaerobiccloseddigestertankfortreatingpalmoilmilleffluent AT sakaikenji pcrbaseddggeandfishanalysisofmethanogensinananaerobiccloseddigestertankfortreatingpalmoilmilleffluent AT ikenoshinya pcrbaseddggeandfishanalysisofmethanogensinananaerobiccloseddigestertankfortreatingpalmoilmilleffluent AT morimasatsugu pcrbaseddggeandfishanalysisofmethanogensinananaerobiccloseddigestertankfortreatingpalmoilmilleffluent AT kazunorinakamura pcrbaseddggeandfishanalysisofmethanogensinananaerobiccloseddigestertankfortreatingpalmoilmilleffluent AT sulaimanalawi pcrbaseddggeandfishanalysisofmethanogensinananaerobiccloseddigestertankfortreatingpalmoilmilleffluent AT hassanmohdali pcrbaseddggeandfishanalysisofmethanogensinananaerobiccloseddigestertankfortreatingpalmoilmilleffluent
_version_	1718441802626760704

PCR-based DGGE and FISH analysis of methanogens in an anaerobic closed digester tank for treating palm oil mill effluent

Ejemplares similares