Culture conditions for the production of a-galactosidase by Aspergillus parasiticus MTCC-2796: a novel source

Aspergillus parasiticus microbial type culture collection (MTCC)-2796, a new source of a-galactosidase is an efficient producer of enzyme in basic medium under submerged fermentation conditions. Maximum a-galactosidase production (156.25 Uml-1) was obtained when the basic medium is supplemented with...

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Autores principales:	Shivam,Kumar, Tripathi,Chandra Prakash Mani, Mishra,Sarad Kumar
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2009
Materias:	Aspergillus parasiticus a-galactosidase culture condition submerged fermentation
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000400004
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spelling	oai:scielo:S0717-345820090004000042010-04-19Culture conditions for the production of a-galactosidase by Aspergillus parasiticus MTCC-2796: a novel sourceShivam,KumarTripathi,Chandra Prakash ManiMishra,Sarad Kumar Aspergillus parasiticus a-galactosidase culture condition submerged fermentation Aspergillus parasiticus microbial type culture collection (MTCC)-2796, a new source of a-galactosidase is an efficient producer of enzyme in basic medium under submerged fermentation conditions. Maximum a-galactosidase production (156.25 Uml-1) was obtained when the basic medium is supplemented with galactose (0.5% w/v) and raffinose (0.5% w/v) as carbon source and yeast extract as nitrogen source. Enzyme production was also enhanced considerably in the presence of wheat bran (1.0% w/v). Enzyme secretion was strongly inhibited by the presence of Hg2+, Cu2+, and Co2+ in the medium and to some extent by Zn2+ and Ni2+, while marginal increase in the enzyme production was observed when Mg2+ and Mn2+ were added in the medium. Among amino acids checked (aparagine, cysteine, glutamine, leucine and proline), glutamine (1 mM) was found to be an enhancer for the enzyme production. The temperature and pH range for the production of enzyme were 25ºC to 35ºC and 6.5 to 7.5, respectively with maximum activity (50 Uml-1) at 30ºC and pH 6.5 under static fermentation condition.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.12 n.4 20092009-10-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000400004en10.4067/S0717-34582009000400004
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Aspergillus parasiticus a-galactosidase culture condition submerged fermentation
spellingShingle	Aspergillus parasiticus a-galactosidase culture condition submerged fermentation Shivam,Kumar Tripathi,Chandra Prakash Mani Mishra,Sarad Kumar Culture conditions for the production of a-galactosidase by Aspergillus parasiticus MTCC-2796: a novel source
description	Aspergillus parasiticus microbial type culture collection (MTCC)-2796, a new source of a-galactosidase is an efficient producer of enzyme in basic medium under submerged fermentation conditions. Maximum a-galactosidase production (156.25 Uml-1) was obtained when the basic medium is supplemented with galactose (0.5% w/v) and raffinose (0.5% w/v) as carbon source and yeast extract as nitrogen source. Enzyme production was also enhanced considerably in the presence of wheat bran (1.0% w/v). Enzyme secretion was strongly inhibited by the presence of Hg2+, Cu2+, and Co2+ in the medium and to some extent by Zn2+ and Ni2+, while marginal increase in the enzyme production was observed when Mg2+ and Mn2+ were added in the medium. Among amino acids checked (aparagine, cysteine, glutamine, leucine and proline), glutamine (1 mM) was found to be an enhancer for the enzyme production. The temperature and pH range for the production of enzyme were 25ºC to 35ºC and 6.5 to 7.5, respectively with maximum activity (50 Uml-1) at 30ºC and pH 6.5 under static fermentation condition.
author	Shivam,Kumar Tripathi,Chandra Prakash Mani Mishra,Sarad Kumar
author_facet	Shivam,Kumar Tripathi,Chandra Prakash Mani Mishra,Sarad Kumar
author_sort	Shivam,Kumar
title	Culture conditions for the production of a-galactosidase by Aspergillus parasiticus MTCC-2796: a novel source
title_short	Culture conditions for the production of a-galactosidase by Aspergillus parasiticus MTCC-2796: a novel source
title_full	Culture conditions for the production of a-galactosidase by Aspergillus parasiticus MTCC-2796: a novel source
title_fullStr	Culture conditions for the production of a-galactosidase by Aspergillus parasiticus MTCC-2796: a novel source
title_full_unstemmed	Culture conditions for the production of a-galactosidase by Aspergillus parasiticus MTCC-2796: a novel source
title_sort	culture conditions for the production of a-galactosidase by aspergillus parasiticus mtcc-2796: a novel source
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2009
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000400004
work_keys_str_mv	AT shivamkumar cultureconditionsfortheproductionofagalactosidasebyaspergillusparasiticusmtcc2796anovelsource AT tripathichandraprakashmani cultureconditionsfortheproductionofagalactosidasebyaspergillusparasiticusmtcc2796anovelsource AT mishrasaradkumar cultureconditionsfortheproductionofagalactosidasebyaspergillusparasiticusmtcc2796anovelsource
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Culture conditions for the production of a-galactosidase by Aspergillus parasiticus MTCC-2796: a novel source

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