Characterization of cellulolytic activities of Bjerkandera adusta and Pycnoporus sanguineus on solid wheat straw medium

Cellulolytic properties of two white rot fungi, Bjerkandera adusta and Pycnoporus sanguineus, cultivated on wheat straw agar medium, were characterized and compared. Optimal growing parameters for maximum enzyme production for both fungi were wheat straw medium pH 5 and 28ºC. B. adusta showed, on th...

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Autores principales:	Quiroz-Castañeda,Rosa E, Balcázar-López,Edgar, Dantán-González,Edgar, Martinez,Alfredo, Folch-Mallol,Jorge, Martínez Anaya,Claudia
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2009
Materias:	B. adusta cellulases P. sanguineus wheat straw xylanases
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000400005
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spelling	oai:scielo:S0717-345820090004000052010-04-19Characterization of cellulolytic activities of Bjerkandera adusta and Pycnoporus sanguineus on solid wheat straw mediumQuiroz-Castañeda,Rosa EBalcázar-López,EdgarDantán-González,EdgarMartinez,AlfredoFolch-Mallol,JorgeMartínez Anaya,Claudia B. adusta cellulases P. sanguineus wheat straw xylanases Cellulolytic properties of two white rot fungi, Bjerkandera adusta and Pycnoporus sanguineus, cultivated on wheat straw agar medium, were characterized and compared. Optimal growing parameters for maximum enzyme production for both fungi were wheat straw medium pH 5 and 28ºC. B. adusta showed, on the 6th day of culture, carboxymethylcellulose (CMC)ase activity levels 1.6 times higher than maximal P. sanguineus activity, achieved on the 8th day. B. adusta supernatants also displayed higher activity levels towards xylan (3.6-fold) compared to those of P. sanguineus. However, enzymes from P. sanguineus were more robust resisting one hour incubation at high temperatures (up to 80ºC), and exhibiting activity and stability in pH range from 2 to 8. Cellulolytic activities, with molecular masses ranging from 25 to 90 kDa, from the two species were detected in zymograms.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.12 n.4 20092009-10-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000400005en10.4067/S0717-34582009000400005
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	B. adusta cellulases P. sanguineus wheat straw xylanases
spellingShingle	B. adusta cellulases P. sanguineus wheat straw xylanases Quiroz-Castañeda,Rosa E Balcázar-López,Edgar Dantán-González,Edgar Martinez,Alfredo Folch-Mallol,Jorge Martínez Anaya,Claudia Characterization of cellulolytic activities of Bjerkandera adusta and Pycnoporus sanguineus on solid wheat straw medium
description	Cellulolytic properties of two white rot fungi, Bjerkandera adusta and Pycnoporus sanguineus, cultivated on wheat straw agar medium, were characterized and compared. Optimal growing parameters for maximum enzyme production for both fungi were wheat straw medium pH 5 and 28ºC. B. adusta showed, on the 6th day of culture, carboxymethylcellulose (CMC)ase activity levels 1.6 times higher than maximal P. sanguineus activity, achieved on the 8th day. B. adusta supernatants also displayed higher activity levels towards xylan (3.6-fold) compared to those of P. sanguineus. However, enzymes from P. sanguineus were more robust resisting one hour incubation at high temperatures (up to 80ºC), and exhibiting activity and stability in pH range from 2 to 8. Cellulolytic activities, with molecular masses ranging from 25 to 90 kDa, from the two species were detected in zymograms.
author	Quiroz-Castañeda,Rosa E Balcázar-López,Edgar Dantán-González,Edgar Martinez,Alfredo Folch-Mallol,Jorge Martínez Anaya,Claudia
author_facet	Quiroz-Castañeda,Rosa E Balcázar-López,Edgar Dantán-González,Edgar Martinez,Alfredo Folch-Mallol,Jorge Martínez Anaya,Claudia
author_sort	Quiroz-Castañeda,Rosa E
title	Characterization of cellulolytic activities of Bjerkandera adusta and Pycnoporus sanguineus on solid wheat straw medium
title_short	Characterization of cellulolytic activities of Bjerkandera adusta and Pycnoporus sanguineus on solid wheat straw medium
title_full	Characterization of cellulolytic activities of Bjerkandera adusta and Pycnoporus sanguineus on solid wheat straw medium
title_fullStr	Characterization of cellulolytic activities of Bjerkandera adusta and Pycnoporus sanguineus on solid wheat straw medium
title_full_unstemmed	Characterization of cellulolytic activities of Bjerkandera adusta and Pycnoporus sanguineus on solid wheat straw medium
title_sort	characterization of cellulolytic activities of bjerkandera adusta and pycnoporus sanguineus on solid wheat straw medium
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2009
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000400005
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Characterization of cellulolytic activities of Bjerkandera adusta and Pycnoporus sanguineus on solid wheat straw medium

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