Degradation of methomyl by the novel bacterial strain Stenotrophomonas maltophilia M1

The use of microorganisms in the degradation and detoxification of many toxic xenobiotics, especially pesticides, is an efficient tool for the decontamination of polluted sites in the environment. A novel bacterial strain (M1) was isolated from several water samples contaminated with methomyl which...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autor principal:	Mohamed,Mervat S
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2009
Materias:	carbamate methomyl plasmid SPE-LC-ESI-MS Stenotrophomonas
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000400006
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0717-34582009000400006
record_format	dspace
spelling	oai:scielo:S0717-345820090004000062010-04-19Degradation of methomyl by the novel bacterial strain Stenotrophomonas maltophilia M1Mohamed,Mervat S carbamate methomyl plasmid SPE-LC-ESI-MS Stenotrophomonas The use of microorganisms in the degradation and detoxification of many toxic xenobiotics, especially pesticides, is an efficient tool for the decontamination of polluted sites in the environment. A novel bacterial strain (M1) was isolated from several water samples contaminated with methomyl which is capable of degrading methomyl pesticide (1000 ppm) in the presence of 0.05% glucose. These water samples were collected from different irrigation sites in Egypt where methomyl is heavily applied. The partial sequence of 16SrRNA gene of the isolate showed the highest similarity to Stenotrophomonas maltophilia. Restriction fragment patterns of isolated plasmid DNA showed that this strain harbours two different plasmids PMa (8Kb) and PMb (5Kb). PMb succeeded to be transferred to Escherichia coli DH5α strain. This transformed strain (M2) acquired the ability to grow in the presence of methomyl (1000 ppm) and 0.05% glucose. So it was deduced that the gene responsible for the degradation process was encoded by this plasmid. The ability of the two strains M1 and M2 to degrade methomyl was detected by using solid phased extraction coupled to capillary liquid chromatography-electrospray ionization-mass spectrometry (SPE-LC-ESI-MS).info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.12 n.4 20092009-10-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000400006en10.4067/S0717-34582009000400006
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	carbamate methomyl plasmid SPE-LC-ESI-MS Stenotrophomonas
spellingShingle	carbamate methomyl plasmid SPE-LC-ESI-MS Stenotrophomonas Mohamed,Mervat S Degradation of methomyl by the novel bacterial strain Stenotrophomonas maltophilia M1
description	The use of microorganisms in the degradation and detoxification of many toxic xenobiotics, especially pesticides, is an efficient tool for the decontamination of polluted sites in the environment. A novel bacterial strain (M1) was isolated from several water samples contaminated with methomyl which is capable of degrading methomyl pesticide (1000 ppm) in the presence of 0.05% glucose. These water samples were collected from different irrigation sites in Egypt where methomyl is heavily applied. The partial sequence of 16SrRNA gene of the isolate showed the highest similarity to Stenotrophomonas maltophilia. Restriction fragment patterns of isolated plasmid DNA showed that this strain harbours two different plasmids PMa (8Kb) and PMb (5Kb). PMb succeeded to be transferred to Escherichia coli DH5α strain. This transformed strain (M2) acquired the ability to grow in the presence of methomyl (1000 ppm) and 0.05% glucose. So it was deduced that the gene responsible for the degradation process was encoded by this plasmid. The ability of the two strains M1 and M2 to degrade methomyl was detected by using solid phased extraction coupled to capillary liquid chromatography-electrospray ionization-mass spectrometry (SPE-LC-ESI-MS).
author	Mohamed,Mervat S
author_facet	Mohamed,Mervat S
author_sort	Mohamed,Mervat S
title	Degradation of methomyl by the novel bacterial strain Stenotrophomonas maltophilia M1
title_short	Degradation of methomyl by the novel bacterial strain Stenotrophomonas maltophilia M1
title_full	Degradation of methomyl by the novel bacterial strain Stenotrophomonas maltophilia M1
title_fullStr	Degradation of methomyl by the novel bacterial strain Stenotrophomonas maltophilia M1
title_full_unstemmed	Degradation of methomyl by the novel bacterial strain Stenotrophomonas maltophilia M1
title_sort	degradation of methomyl by the novel bacterial strain stenotrophomonas maltophilia m1
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2009
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000400006
work_keys_str_mv	AT mohamedmervats degradationofmethomylbythenovelbacterialstrainstenotrophomonasmaltophiliam1
_version_	1718441804671483904

Degradation of methomyl by the novel bacterial strain Stenotrophomonas maltophilia M1

Ejemplares similares