Expression of SOD transgene in pepper confer stress tolerance and improve shoot regeneration

The objective of this work was to study the stress tolerance and regeneration capability of transgenic pepper plants carrying a sod gene, encoding a tomato chloroplast-localized Cu/Zn SOD protein. The expression of the sod gene was confirmed by enzymatic staining following polyacrylamide gel electro...

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Autores principales:	Chatzidimitriadou,Kiriaki, Nianiou-Obeidat,Irini, Madesis,Panagiotis, Perl-Treves,Rafael, Tsaftaris,Athanasios
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2009
Materias:	Capsicum annum L. drought stress MV regeneration SOD
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000400007
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spelling	oai:scielo:S0717-345820090004000072010-04-19Expression of SOD transgene in pepper confer stress tolerance and improve shoot regenerationChatzidimitriadou,KiriakiNianiou-Obeidat,IriniMadesis,PanagiotisPerl-Treves,RafaelTsaftaris,Athanasios Capsicum annum L. drought stress MV regeneration SOD The objective of this work was to study the stress tolerance and regeneration capability of transgenic pepper plants carrying a sod gene, encoding a tomato chloroplast-localized Cu/Zn SOD protein. The expression of the sod gene was confirmed by enzymatic staining following polyacrylamide gel electrophoresis (PAGE), revealing a ‘novel’ band, which could represent a heterodimeric enzyme. Transgenic T1 and T2 progeny plants were exposed to different oxidative stresses including Methyl viologen (MV) and drought and found to have an increased resistance to oxidative damage. Furthermore, the SOD carrying transgenic pepper plants showed increased levels of regeneration efficiency compared to the wild type pepper plants. Pepper is a recalcitrant species in terms of its in vitro regeneration ability but it could be extremely useful for the development of pharmaceuticals. This approach enables the extent use of pepper for genetic transformation and the production of high valuable products in plants particularly the large fruit varieties.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.12 n.4 20092009-10-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000400007en10.4067/S0717-34582009000400007
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Capsicum annum L. drought stress MV regeneration SOD
spellingShingle	Capsicum annum L. drought stress MV regeneration SOD Chatzidimitriadou,Kiriaki Nianiou-Obeidat,Irini Madesis,Panagiotis Perl-Treves,Rafael Tsaftaris,Athanasios Expression of SOD transgene in pepper confer stress tolerance and improve shoot regeneration
description	The objective of this work was to study the stress tolerance and regeneration capability of transgenic pepper plants carrying a sod gene, encoding a tomato chloroplast-localized Cu/Zn SOD protein. The expression of the sod gene was confirmed by enzymatic staining following polyacrylamide gel electrophoresis (PAGE), revealing a ‘novel’ band, which could represent a heterodimeric enzyme. Transgenic T1 and T2 progeny plants were exposed to different oxidative stresses including Methyl viologen (MV) and drought and found to have an increased resistance to oxidative damage. Furthermore, the SOD carrying transgenic pepper plants showed increased levels of regeneration efficiency compared to the wild type pepper plants. Pepper is a recalcitrant species in terms of its in vitro regeneration ability but it could be extremely useful for the development of pharmaceuticals. This approach enables the extent use of pepper for genetic transformation and the production of high valuable products in plants particularly the large fruit varieties.
author	Chatzidimitriadou,Kiriaki Nianiou-Obeidat,Irini Madesis,Panagiotis Perl-Treves,Rafael Tsaftaris,Athanasios
author_facet	Chatzidimitriadou,Kiriaki Nianiou-Obeidat,Irini Madesis,Panagiotis Perl-Treves,Rafael Tsaftaris,Athanasios
author_sort	Chatzidimitriadou,Kiriaki
title	Expression of SOD transgene in pepper confer stress tolerance and improve shoot regeneration
title_short	Expression of SOD transgene in pepper confer stress tolerance and improve shoot regeneration
title_full	Expression of SOD transgene in pepper confer stress tolerance and improve shoot regeneration
title_fullStr	Expression of SOD transgene in pepper confer stress tolerance and improve shoot regeneration
title_full_unstemmed	Expression of SOD transgene in pepper confer stress tolerance and improve shoot regeneration
title_sort	expression of sod transgene in pepper confer stress tolerance and improve shoot regeneration
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2009
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000400007
work_keys_str_mv	AT chatzidimitriadoukiriaki expressionofsodtransgeneinpepperconferstresstoleranceandimproveshootregeneration AT nianiouobeidatirini expressionofsodtransgeneinpepperconferstresstoleranceandimproveshootregeneration AT madesispanagiotis expressionofsodtransgeneinpepperconferstresstoleranceandimproveshootregeneration AT perltrevesrafael expressionofsodtransgeneinpepperconferstresstoleranceandimproveshootregeneration AT tsaftarisathanasios expressionofsodtransgeneinpepperconferstresstoleranceandimproveshootregeneration
_version_	1718441805018562560

Expression of SOD transgene in pepper confer stress tolerance and improve shoot regeneration

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